https://doi.org/10.4081/jear.2016.5699

Effect of Citrullus colocynthis L. (Cucurbitaceae) agglutinin on gene expression of caspases in Ectomyelois ceratoniae Zeller (Lepidoptera: Crambidae)

Vol. 48 No. 3 (2016)
Submitted: 20 December 2015
Accepted: 29 March 2016
Published: 19 December 2016
Ectomyelois ceratoniae, Citrullus colocynthis, lectin, life table.
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Authors

S. Ramzi
samar.ramzi@live.com
Plant Protection Research Department, Horticultural Science Research Institute, Tea Research Center, Agricultural Research Education and Extension Organization (AREEO), Lahijan, Iran, Islamic Republic of.
A. Sahragard
Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran, Islamic Republic of.
J.J. Sendi
Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran, Islamic Republic of.
A. Aalami
Department of Agronomy and Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran, Islamic Republic of.
The larvae of Ectomyelois ceratoniae Zeller were reared on the artificial diets containing 2% of Citrullus colocynthis L. (Cucurbitaceae) lectin and control to find any changes in gene expression of caspases. Specific primers were designed to amplify genes of six found caspases including Caspase 1, 2, 3, 4, 5 and 6. RNA of both treatments were extracted and cDNA molecules were synthesized prior to gene expression. Results of quantitative real-time polymerase chain reaction revealed that expression of all caspases in the fed larvae by 2% of lectin increased in comparison with control except for caspase 1 as 2.99, 5.7, 17.13, 23.18, 10.44-fold. These results are consistent with our previous studies on the lower activities of digestive enzymes that are due to death of midgut cells followed by binding of lectin molecule.

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How to Cite

Ramzi, S., Sahragard, A., Sendi, J., & Aalami, A. (2016). Effect of Citrullus colocynthis L. (Cucurbitaceae) agglutinin on gene expression of caspases in Ectomyelois ceratoniae Zeller (Lepidoptera: Crambidae). Journal of Entomological and Acarological Research, 48(3), 304–307. https://doi.org/10.4081/jear.2016.5699

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