https://doi.org/10.4081/jear.2022.10316

Infestation of Apis mellifera workers and larvae with Varroa destructor affects gut bacterial diversity

Vol. 54 No. 1 (2022)
Submitted: 6 December 2021
Accepted: 1 February 2022
Published: 1 August 2022
Apis mellifera, midgut bacteria, Varroa destructor, 16S rRNA
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Authors

F.F. Abuldahab
Entomology Department, Faculty of Science, Benha University, Benha, Egypt.
M.F. Abd-ElAziz
Entomology Department, Faculty of Science, Benha University, Benha, Egypt.
Y.A. El-Sayed
Entomology Department, Faculty of Science, Benha University, Benha, Egypt.
M.S. Abdallah
Entomology Department, Faculty of Science, Benha University, Benha, Egypt.
S.H. Mahmoud
dr.shaymaahussein@science.menofia.edu.eg
Zoology Department, Faculty of Science, Menoufia University, Menoufia, Egypt.

The present work aims to investigate the midgut bacteria of honey bee (Apis mellifera lamarckii) workers and larvae infested with Varroa destructor. To achieve this task, uninfested and infested honey bee larvae and workers with the parasitic mites were obtained from natural bees apiaries in Qaliubiya Governorate, Egypt. Aerobic bacteria were isolated from the midgut of uninfested and infested A. mellifera on nutrient agar medium and identified using a culture-dependent isolation along with 16S rRNA sequences. The results revealed that both uninfested and V. destructor infested workers and larvae harbored gut bacteria belonging to Gamma-proteobacteria strains. The uninfested workers contained Enterobacter cloacae and Serratia nematodiphila, while V. destructor infested workers harbored Enterobacter species. Uninfested larvae contained E. hormaechei and Klebsiella variicola. V. destructor infested larvae harbored K. oxytoca as a result of infection. We concluded that the honeybee microbiome composition is likely to be impacted through isolates variation by stage and diversity disruption of isolates by V. destructor infection.

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How to Cite

Abuldahab, F., Abd-ElAziz, M. ., El-Sayed, Y. ., Abdallah, M. ., & Mahmoud, S. . (2022). Infestation of <em>Apis mellifera</em> workers and larvae with <em>Varroa destructor</em> affects gut bacterial diversity. Journal of Entomological and Acarological Research, 54(1). https://doi.org/10.4081/jear.2022.10316
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