Characterization of microbial communities in pest colonized books by molecular biology tools


Submitted: 4 February 2012
Accepted: 4 February 2012
Published: 20 August 2011
Abstract Views: 1611
PDF: 924
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Authors

This work presents the identification of bacteria and fungi colonies in insect infesting books, by cultural-independent methodologies based on molecular biology techniques. Microbial genomic DNA extraction, in vitro amplification of specific target sequences by polymerase chain reactions (PCR), sequencing and sequence analysis were performed. These procedures minimized the samples amount, optimized the diagnostic studies on bacteria and fungi colonization and allowed the identification of many species also in complex microbial consortia. The molecular techniques for sure accomplish and integrate the microbiological standard methods (in vitro culture) and morphological analyses (OM, SEM, CLSM), in order to understand the role of microorganisms in bio-deterioration of cultural assets. This monitoring is also indispensable to shed light on the risk for visitors and/or professionals to contract potential illnesses within indoor environments.

Palla, F. (2011). Characterization of microbial communities in pest colonized books by molecular biology tools. Journal of Entomological and Acarological Research, 43(2), 61–67. https://doi.org/10.4081/jear.2011.61

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