Development of an innovative microcantilever-based biosensor for 17β-estradiol detection in bovine muscles: preliminary results

  • Danilo Pitardi | d.pitardi@alice.it Istituto Zooprofilattico Sperimentale di Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154, Torino, Italy.
  • Marzia Pezzolato Istituto Zooprofilattico Sperimentale di Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154, Torino, Italy.
  • Guia Richelmi Istituto Zooprofilattico Sperimentale di Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154, Torino, Italy.
  • Marilena Gili Istituto Zooprofilattico Sperimentale di Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154, Torino, Italy.
  • Daniele Di Corcia Centro Regionale Antidoping, Regione Gonzole 10/1, 10043 Orbassano (To), Italy.
  • Niccolò Ciprianetti Politecnico di Torino - LATEMAR Unit, Department of Applied Science and Technology, Corso Duca degli Abruzzi 24, I-10129 Torino, Italy, Italy.
  • Ivan Ferrante Laboratorio di Tecnologie Elettrobiochimiche Miniaturizzate per l’Analisi e la Ricerca - Politecnico di Torino, Corso Duca degli Abruzzi 24, I-10129 Torino, Italy.
  • Karin Santoro Laboratorio di Tecnologie Elettrobiochimiche Miniaturizzate per l’Analisi e la Ricerca - Politecnico di Torino, Corso Duca degli Abruzzi 24, I-10129 Torino, Italy.
  • Carlo Ricciardi Laboratorio di Tecnologie Elettrobiochimiche Miniaturizzate per l’Analisi e la Ricerca - Politecnico di Torino, Corso Duca degli Abruzzi 24, I-10129 Torino, Italy.
  • Elena Bozzetta Istituto Zooprofilattico Sperimentale di Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 10154, Torino, Italy.

Abstract

17β-estradiol is the most powerful substance with estrogenic effect, commonly used as illegal growth promoter in livestock production. To avoid health risks for consumers, sensitive, reliable and low-cost methods for quantification of extremely low concentrations of such carcinogenic residues in food are needed. Antibody-immobilised microcantilever resonators were proposed as innovative biosensors able to quantify an adsorbed target mass thanks to a shift in resonance frequency. Furthermore, the quantification of masses on the order of few picograms has recently shown to be successfully achievable with very high precision. In this study, we analysed the performance of our microcantilever sensors using extracted samples of bovine muscle from experimental animals, containing variable concentrations of 17β-estradiol (HPLC-MS/MS tested). Preliminary data showed that treated animals are correctly revealed, exhibiting large negative frequency shifts. More experiments, though, are needed to obtain a correct quantification of 17β-estradiol concentration.

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Published
2013-06-10
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Section
Short Communications
Keywords:
17β-Estradiol detection, Bovine muscle, Microcantilever, Biosensor
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How to Cite
1.
Pitardi D, Pezzolato M, Richelmi G, Gili M, Di Corcia D, Ciprianetti N, Ferrante I, Santoro K, Ricciardi C, Bozzetta E. Development of an innovative microcantilever-based biosensor for 17β-estradiol detection in bovine muscles: preliminary results. Ital J Food Safety [Internet]. 2013Jun.10 [cited 2021Oct.16];2(1):e12. Available from: https://pagepressjournals.org/index.php/ijfs/article/view/ijfs.2013.e12