Extraction and identification of microplastics from mussels: Method development and preliminary results

  • Raffaelina Mercogliano Department of Veterinary Medicine and Animal Production, University of Naples, Italy.
  • Serena Santonicola | serena.santonicola@unina.it Department of Medicine and Health Sciences, University of Molise, Campobasso, Italy.
  • Gennaro Raimo Department of Medicine and Health Sciences, University of Molise, Campobasso, Italy.
  • Maurizio Gasperi Department of Medicine and Health Sciences, University of Molise, Campobasso, Italy.
  • Giampaolo Colavita Department of Medicine and Health Sciences, University of Molise, Campobasso, Italy.


Microplastics (MPs) are an emerging threat to marine ecosystems. One of the primary environmental risks is their bioavailability for aquatic organisms. Some fish and bivalves are of particular interest because their feeding strategies expose them to particles present in the water column. The aim of the study was to assess an extraction method in order to isolate and quantify MPs from fish gastrointestinal tract (n.8) and muscle (n.4), and bivalves (n.8) samples. The accuracy of the method was assessed through the calculation of the recovery percentage in samples spiked with a known number of MPs using microscopic observation. Successively, the extraction was preliminarily applied on n.20 mussels collected from mariculture plants of the Tyrrhenian and the Adriatic Sea. The results of the digestion protocol showed an average extraction yield of 80% in fish gastrointestinal tracts, 90% in fish muscle samples, and 95% in mussels. Preliminary analysis carried out on farmed mussels showed an average abundance of 3.8 items/individual, and 0.5 items/g of tissue, among those black, was the most represented color.



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Avio CG, Gorbi S, Regoli F, 2015. Experimental development of a new protocol for extraction and characterization of microplastics in fish tissues: first observations in commercial species from Adriatic Sea. Mar Environ Res 111: 18-26. DOI: https://doi.org/10.1016/j.marenvres.2015.06.014

Barboza LGA, Vethaak AD, Lavorante BR, Lundebye AK, Guilhermino L, 2018. Marine microplastic debris: An emerging issue for food security, food safety and human health. Mar Pollut Bull 133: 336-348. DOI: https://doi.org/10.1016/j.marpolbul.2018.05.047

Bessa F, Frias J, Kögel T, Lusher A, Andrade JM, Antunes J, Pedrotti ML, 2019. Harmonized protocol for monitoring microplastics in biota. Deliverable 4.3.

Borme D, Tirelli V, Brandt SB, Umani SF, Arneri, E, 2009. Diet of Engraulis encrasicolus in the northern Adriatic Sea (Mediterranean): ontogenetic changes and feeding selectivity. Mar Ecol Prog Ser 392: 193-209. DOI: https://doi.org/10.3354/meps08214

Catarino AI, Thompson R, Sanderson W, Henry TB, 2017. Development and optimization of a standard method for extraction of microplastics in mussels by enzyme digestion of soft tissues. Environ Toxicol Chem 36: 947-951. DOI: https://doi.org/10.1002/etc.3608

Digka N, Tsangaris C, Torre M, Anastasopoulou A, Zeri C., 2018. Microplastics in mussels and fish from the Northern Ionian Sea. Mar Pollut Bull 135: 30-40. DOI: https://doi.org/10.1016/j.marpolbul.2018.06.063

EFSA Panel on Contaminants in the Food Chain (CONTAM), 2016. Presence of microplastics and nanoplastics in food, with particular focus on seafood. Efsa J 14: e04501. DOI: https://doi.org/10.2903/j.efsa.2016.4501

Foekema EM, De Gruijter C, Mergia MT, van Franeker JA, Murk AJ, Koelmans AA, 2013. Plastic in north sea fish. Environ Sci Technol 47: 8818-8824. DOI: https://doi.org/10.1021/es400931b

Gomiero A, Øysæd KB, Agustsson T, van Hoytema N, van Thiel T, Grati F, 2019. First record of characterization, concentration and distribution of microplastics in coastal sediments of an urban fjord in south west Norway using a thermal degradation method. Chemosphere 227: 705-714. DOI: https://doi.org/10.1016/j.chemosphere.2019.04.096

Güven O, Gökdağ K, Jovanović B, Kıdeyş AE, 2017. Microplastic litter composition of the Turkish territorial waters of the Mediterranean Sea, and its occurrence in the gastrointestinal tract of fish. Environ Pollut 223, 286-294. DOI: https://doi.org/10.1016/j.envpol.2017.01.025

Karami A, Golieskardi A, Ho YB, Larat V, Salamatinia B, 2017. Microplastics in eviscerated flesh and excised organs of dried fish. Sci Rep 7: 1-9. DOI: https://doi.org/10.1038/s41598-017-05828-6

Kolandhasamy P, Su L, Li J, Qu X, Jabeen K, Shi H, 2018. Adherence of microplastics to soft tissue of mussels: a novel way to uptake microplastics beyond ingestion. Sci Total Environ, 610: 635-640. DOI: https://doi.org/10.1016/j.scitotenv.2017.08.053

Li J, Green C, Reynolds A, Shi H, Rotchell JM, 2018. Microplastics in mussels sampled from coastal waters and supermarkets in the United Kingdom. Environ Pollut, 241: 35-44. DOI: https://doi.org/10.1016/j.envpol.2018.05.038

Li J, Lusher AL, Rotchell JM, Deudero S, Turra A, Bråte ILN, Shi H, 2019. Using mussel as a global bioindicator of coastal microplastic pollution. Environ Pollut, 244: 522-533. DOI: https://doi.org/10.1016/j.envpol.2018.10.032

Li J, Qu X, Su L, Zhang W, Yang D, Kolandhasamy P, Shi H, 2016. Microplastics in mussels along the coastal waters of China. Environ Pollut, 214: 177-184. DOI: https://doi.org/10.1016/j.envpol.2016.04.012

Lusher A, 2015. Microplastics in the marine environment: distribution, interactions and effects. In Marine anthropogenic litter (pp. 245-307). Springer, Cham. DOI: https://doi.org/10.1007/978-3-319-16510-3_10

Lusher AL, Welden NA, Sobral P, Cole M, 2017. Sampling, isolating and identifying microplastics ingested by fish and invertebrates. Anal Methods 9: 1346-1360. DOI: https://doi.org/10.1039/C6AY02415G

Mathalon A, Hill P, 2014. Microplastic fibers in the intertidal ecosystem surrounding Halifax Harbor, Nova Scotia. Mar Pollut Bull 81: 69-79. DOI: https://doi.org/10.1016/j.marpolbul.2014.02.018

Mercogliano R, Avio CG, Regoli F, Anastasio A, Colavita G, Santonicola S, 2020. Occurrence of Microplastics in Commercial Seafood under the Perspective of the Human Food Chain. A Review. J Agr Food Chem, 68: 5296-5301. DOI: https://doi.org/10.1021/acs.jafc.0c01209

Mizraji R, Ahrendt C, Perez-Venegas D, Vargas J, Pulgar J, Aldana M, Galbán-Malagón C, 2017. Is the feeding type related with the content of microplastics in intertidal fish gut?. Mar Pollut Bull. 116: 498-500. DOI: https://doi.org/10.1016/j.marpolbul.2017.01.008

Phuong NN, Poirier L, Pham QT, Lagarde F, Zalouk-Vergnoux A, 2018. Factors influencing the microplastic contamination of bivalves from the French Atlantic coast: location, season and/or mode of life?. Mar Pollut Bull, 129: 664-674. DOI: https://doi.org/10.1016/j.marpolbul.2017.10.054

Qu X, Su L, Li H, Liang M, Shi H, 2018. Assessing the relationship between the abundance and properties of microplastics in water and in mussels. Sci Total Environ, 621: 679-686. DOI: https://doi.org/10.1016/j.scitotenv.2017.11.284

Renzi M, Guerranti C, Blašković A, 2018. Microplastic contents from maricultured and natural mussels. Mar Pollut Bull, 131: 248-251. DOI: https://doi.org/10.1016/j.marpolbul.2018.04.035

Rummel CD, Löder MG, Fricke NF, Lang T, Griebeler EM, Janke M, Gerdts G, 2016. Plastic ingestion by pelagic and demersal fish from the North Sea and Baltic Sea. Mar Pollut Bull, 102: 134-141. DOI: https://doi.org/10.1016/j.marpolbul.2015.11.043

Santonicola S, García Ibarra V, Sendón R, Mercogliano R, Rodríguez-Bernaldo de Quirós A, 2017. Antimicrobial films based on chitosan and methylcellulose containing natamycin for active packaging applications. Coatings, 7: 177. DOI: https://doi.org/10.3390/coatings7100177

Setälä O, Norkko J, Lehtiniemi M, 2016. Feeding type affects microplastic ingestion in a coastal invertebrate community. Mar Pollut Bull, 102: 95-101. DOI: https://doi.org/10.1016/j.marpolbul.2015.11.053

Shim WJ, Hong SH, Eo S, 2018. Marine microplastics: abundance, distribution, and composition. In Microplastic Contamination in Aquatic Environments (pp. 1-26). Elsevier DOI: https://doi.org/10.1016/B978-0-12-813747-5.00001-1

Silva AB, Bastos AS, Justino CI, da Costa JP, Duarte AC, Rocha-Santos TA, 2018. Microplastics in the environment: Challenges in analytical chemistry-A review. Anal Chim Acta, 1017: 1-19. DOI: https://doi.org/10.1016/j.aca.2018.02.043

Toussaint B, Raffael B, Angers-Loustau A, Gilliland D, Kestens V, Petrillo M, Van den Eede G, 2019. Review of micro-and nanoplastic contamination in the food chain. Food Addit Contam: Part A, 36: 639-673. DOI: https://doi.org/10.1080/19440049.2019.1583381

Original Articles
Microplastics, Digestion method, Engraulis encrasiculos,, Mytilus galloprovincialis
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How to Cite
Mercogliano R, Santonicola S, Raimo G, Gasperi M, Colavita G. Extraction and identification of microplastics from mussels: Method development and preliminary results. Ital J Food Safety [Internet]. 2021Mar.11 [cited 2021Jul.29];10(1). Available from: https://pagepressjournals.org/index.php/ijfs/article/view/9264