Extraction and identification of microplastics from mussels: Method development and preliminary results

Submitted: 17 July 2020
Accepted: 1 February 2021
Published: 11 March 2021
Abstract Views: 1670
PDF: 865
HTML: 6
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

Microplastics (MPs) are an emerging threat to marine ecosystems. One of the primary environmental risks is their bioavailability for aquatic organisms. Some fish and bivalves are of particular interest because their feeding strategies expose them to particles present in the water column. The aim of the study was to assess an extraction method in order to isolate and quantify MPs from fish gastrointestinal tract (n.8) and muscle (n.4), and bivalves (n.8) samples. The accuracy of the method was assessed through the calculation of the recovery percentage in samples spiked with a known number of MPs using microscopic observation. Successively, the extraction was preliminarily applied on n.20 mussels collected from mariculture plants of the Tyrrhenian and the Adriatic Sea. The results of the digestion protocol showed an average extraction yield of 80% in fish gastrointestinal tracts, 90% in fish muscle samples, and 95% in mussels. Preliminary analysis carried out on farmed mussels showed an average abundance of 3.8 items/individual, and 0.5 items/g of tissue, among those black, was the most represented color.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

Citations

Avio CG, Gorbi S, Regoli F, 2015. Experimental development of a new protocol for extraction and characterization of microplastics in fish tissues: first observations in commercial species from Adriatic Sea. Mar Environ Res 111: 18-26. DOI: https://doi.org/10.1016/j.marenvres.2015.06.014
Barboza LGA, Vethaak AD, Lavorante BR, Lundebye AK, Guilhermino L, 2018. Marine microplastic debris: An emerging issue for food security, food safety and human health. Mar Pollut Bull 133: 336-348. DOI: https://doi.org/10.1016/j.marpolbul.2018.05.047
Bessa F, Frias J, Kögel T, Lusher A, Andrade JM, Antunes J, Pedrotti ML, 2019. Harmonized protocol for monitoring microplastics in biota. Deliverable 4.3.
Borme D, Tirelli V, Brandt SB, Umani SF, Arneri, E, 2009. Diet of Engraulis encrasicolus in the northern Adriatic Sea (Mediterranean): ontogenetic changes and feeding selectivity. Mar Ecol Prog Ser 392: 193-209. DOI: https://doi.org/10.3354/meps08214
Catarino AI, Thompson R, Sanderson W, Henry TB, 2017. Development and optimization of a standard method for extraction of microplastics in mussels by enzyme digestion of soft tissues. Environ Toxicol Chem 36: 947-951. DOI: https://doi.org/10.1002/etc.3608
Digka N, Tsangaris C, Torre M, Anastasopoulou A, Zeri C., 2018. Microplastics in mussels and fish from the Northern Ionian Sea. Mar Pollut Bull 135: 30-40. DOI: https://doi.org/10.1016/j.marpolbul.2018.06.063
EFSA Panel on Contaminants in the Food Chain (CONTAM), 2016. Presence of microplastics and nanoplastics in food, with particular focus on seafood. Efsa J 14: e04501. DOI: https://doi.org/10.2903/j.efsa.2016.4501
Foekema EM, De Gruijter C, Mergia MT, van Franeker JA, Murk AJ, Koelmans AA, 2013. Plastic in north sea fish. Environ Sci Technol 47: 8818-8824. DOI: https://doi.org/10.1021/es400931b
Gomiero A, Øysæd KB, Agustsson T, van Hoytema N, van Thiel T, Grati F, 2019. First record of characterization, concentration and distribution of microplastics in coastal sediments of an urban fjord in south west Norway using a thermal degradation method. Chemosphere 227: 705-714. DOI: https://doi.org/10.1016/j.chemosphere.2019.04.096
Güven O, Gökdağ K, Jovanović B, Kıdeyş AE, 2017. Microplastic litter composition of the Turkish territorial waters of the Mediterranean Sea, and its occurrence in the gastrointestinal tract of fish. Environ Pollut 223, 286-294. DOI: https://doi.org/10.1016/j.envpol.2017.01.025
Karami A, Golieskardi A, Ho YB, Larat V, Salamatinia B, 2017. Microplastics in eviscerated flesh and excised organs of dried fish. Sci Rep 7: 1-9. DOI: https://doi.org/10.1038/s41598-017-05828-6
Kolandhasamy P, Su L, Li J, Qu X, Jabeen K, Shi H, 2018. Adherence of microplastics to soft tissue of mussels: a novel way to uptake microplastics beyond ingestion. Sci Total Environ, 610: 635-640. DOI: https://doi.org/10.1016/j.scitotenv.2017.08.053
Li J, Green C, Reynolds A, Shi H, Rotchell JM, 2018. Microplastics in mussels sampled from coastal waters and supermarkets in the United Kingdom. Environ Pollut, 241: 35-44. DOI: https://doi.org/10.1016/j.envpol.2018.05.038
Li J, Lusher AL, Rotchell JM, Deudero S, Turra A, Bråte ILN, Shi H, 2019. Using mussel as a global bioindicator of coastal microplastic pollution. Environ Pollut, 244: 522-533. DOI: https://doi.org/10.1016/j.envpol.2018.10.032
Li J, Qu X, Su L, Zhang W, Yang D, Kolandhasamy P, Shi H, 2016. Microplastics in mussels along the coastal waters of China. Environ Pollut, 214: 177-184. DOI: https://doi.org/10.1016/j.envpol.2016.04.012
Lusher A, 2015. Microplastics in the marine environment: distribution, interactions and effects. In Marine anthropogenic litter (pp. 245-307). Springer, Cham. DOI: https://doi.org/10.1007/978-3-319-16510-3_10
Lusher AL, Welden NA, Sobral P, Cole M, 2017. Sampling, isolating and identifying microplastics ingested by fish and invertebrates. Anal Methods 9: 1346-1360. DOI: https://doi.org/10.1039/C6AY02415G
Mathalon A, Hill P, 2014. Microplastic fibers in the intertidal ecosystem surrounding Halifax Harbor, Nova Scotia. Mar Pollut Bull 81: 69-79. DOI: https://doi.org/10.1016/j.marpolbul.2014.02.018
Mercogliano R, Avio CG, Regoli F, Anastasio A, Colavita G, Santonicola S, 2020. Occurrence of Microplastics in Commercial Seafood under the Perspective of the Human Food Chain. A Review. J Agr Food Chem, 68: 5296-5301. DOI: https://doi.org/10.1021/acs.jafc.0c01209
Mizraji R, Ahrendt C, Perez-Venegas D, Vargas J, Pulgar J, Aldana M, Galbán-Malagón C, 2017. Is the feeding type related with the content of microplastics in intertidal fish gut?. Mar Pollut Bull. 116: 498-500. DOI: https://doi.org/10.1016/j.marpolbul.2017.01.008
Phuong NN, Poirier L, Pham QT, Lagarde F, Zalouk-Vergnoux A, 2018. Factors influencing the microplastic contamination of bivalves from the French Atlantic coast: location, season and/or mode of life?. Mar Pollut Bull, 129: 664-674. DOI: https://doi.org/10.1016/j.marpolbul.2017.10.054
Qu X, Su L, Li H, Liang M, Shi H, 2018. Assessing the relationship between the abundance and properties of microplastics in water and in mussels. Sci Total Environ, 621: 679-686. DOI: https://doi.org/10.1016/j.scitotenv.2017.11.284
Renzi M, Guerranti C, Blašković A, 2018. Microplastic contents from maricultured and natural mussels. Mar Pollut Bull, 131: 248-251. DOI: https://doi.org/10.1016/j.marpolbul.2018.04.035
Rummel CD, Löder MG, Fricke NF, Lang T, Griebeler EM, Janke M, Gerdts G, 2016. Plastic ingestion by pelagic and demersal fish from the North Sea and Baltic Sea. Mar Pollut Bull, 102: 134-141. DOI: https://doi.org/10.1016/j.marpolbul.2015.11.043
Santonicola S, García Ibarra V, Sendón R, Mercogliano R, Rodríguez-Bernaldo de Quirós A, 2017. Antimicrobial films based on chitosan and methylcellulose containing natamycin for active packaging applications. Coatings, 7: 177. DOI: https://doi.org/10.3390/coatings7100177
Setälä O, Norkko J, Lehtiniemi M, 2016. Feeding type affects microplastic ingestion in a coastal invertebrate community. Mar Pollut Bull, 102: 95-101. DOI: https://doi.org/10.1016/j.marpolbul.2015.11.053
Shim WJ, Hong SH, Eo S, 2018. Marine microplastics: abundance, distribution, and composition. In Microplastic Contamination in Aquatic Environments (pp. 1-26). Elsevier DOI: https://doi.org/10.1016/B978-0-12-813747-5.00001-1
Silva AB, Bastos AS, Justino CI, da Costa JP, Duarte AC, Rocha-Santos TA, 2018. Microplastics in the environment: Challenges in analytical chemistry-A review. Anal Chim Acta, 1017: 1-19. DOI: https://doi.org/10.1016/j.aca.2018.02.043
Toussaint B, Raffael B, Angers-Loustau A, Gilliland D, Kestens V, Petrillo M, Van den Eede G, 2019. Review of micro-and nanoplastic contamination in the food chain. Food Addit Contam: Part A, 36: 639-673. DOI: https://doi.org/10.1080/19440049.2019.1583381

How to Cite

1.
Mercogliano R, Santonicola S, Raimo G, Gasperi M, Colavita G. Extraction and identification of microplastics from mussels: Method development and preliminary results. Ital J Food Safety [Internet]. 2021 Mar. 11 [cited 2024 Nov. 23];10(1). Available from: https://www.pagepressjournals.org/ijfs/article/view/9264