Application of the Micro Biological Survey analytical method for the determination of bacterial load in cow raw milk

  • Alessandra Cornacchia | a.cornacchia@izs.it Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise G. Caporale, Teramo, Italy.
  • Maria Antonietta Saletti Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise G. Caporale, Teramo, Italy.
  • Violeta Di Marzio Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise G. Caporale, Teramo, Italy.
  • Romolo Salini Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise G. Caporale, Teramo, Italy.
  • Cristina Marfoglia Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise G. Caporale, Teramo, Italy.
  • Elga Tieri Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise G. Caporale, Teramo, Italy.
  • Nicola D’Alterio Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise G. Caporale, Teramo, Italy.
  • Nicla Marri Istituto Zooprofilattico Sperimentale del Lazio e della Toscana M. Aleandri, Rome, Italy.
  • Francesca Losito Istituto Nazionale Biostrutture e Biosistemi – Consorzio Interuniversitario, Rome, Italy.
  • Alyexandra Arienzo Dipartimento di Scienze, Università degli Studi Roma Tre, Rome, Italy.
  • Lorenza Murgia Dipartimento di Scienze, Università degli Studi Roma Tre, Rome, Italy.
  • Giovanni Antonini Istituto Nazionale Biostrutture e Biosistemi – Consorzio Interuniversitario, Rome; Dipartimento di Scienze, Università degli Studi Roma Tre, Rome, Italy.
  • Simonetta Amatiste Istituto Zooprofilattico Sperimentale del Lazio e della Toscana M. Aleandri, Rome, Italy.
  • Loris Leboffe Istituto Zooprofilattico Sperimentale del Lazio e della Toscana M. Aleandri, Rome; Dipartimento di Scienze, Università degli Studi Roma Tre, Rome, Italy.
  • Francesco Pomilio Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise G. Caporale, Teramo, Italy.

Abstract

The aim of this study was to evaluate the performance of “Micro Biological Survey – MBS Test” in the enumeration of bacterial load in cow raw milk. The MBS test is based on a colorimetric method recently developed and patented by “Roma Tre” University, Italy. The evaluation of the performance of the MBS method was carried out by comparison with plate count at 30°C (gold standard) and flow cytometry. Thirteen independent set of experiments were performed analyzing a total of 104 samples of cow raw milk with the selected methods. Results obtained using the MBS method are comparable with those obtained with the plate count method at 30°C (CFU/mL) and flow cytometry technology; in particular, the results obtained with the MBS method are very close to plate count’s at 30°C. On the other hand, there are statistically significant differences between these two methods’ and flow cytometry technology’s results that could be due to the different experimental conditions.

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References

Antonini G., Malatesta F., Sarti P., 2007. Twenty-five years of cytochrome oxidase research in Rome with Maurizio Brunori. IUBMB Life, 59: 570-577. DOI: https://doi.org/10.1080/15216540701256078

Balouiri M., Sadiki M., Ibnsouda S. K., 2016. Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis, Volume 6, Issue 2, April 2016, Pages 71-79. DOI: https://doi.org/10.1016/j.jpha.2015.11.005

Bottini G., Losito F., De Ascenti A., Priolisi F.R., Mari A., Antonini G., 2011. Validation of the Micro Biological Survey Method for Total Viable Count and E. coli in Food Samples. American Journal of Food Technology, 6: 951-962. DOI: https://doi.org/10.3923/ajft.2011.951.962

Feinberg, M., D. Sohier and J.F. David, 2009. Validation of an alternative method for countin Enterobacteriaceae in foods based on accuracy profile. J. AOAC Int., 2: 527-537. DOI: https://doi.org/10.1093/jaoac/92.2.527

Italian Ministerial Decree March 26, 1992. Implementation of decision n. 91/180 / EEC concerning the establishment of methods of analysis and testing relating to raw milk and heat-treated milk. (GU General Series No. 90 of 16-04-1992 - Ordinary Supplement No. 67).

IZS Lombardia ed Emilia Romagna (IZSLER), Dip. Sanità Pubblica Veterinaria “Bruno Ubertini”, 2013. Aggiornamento dell'equazione di conversione per la determinazione della carica batterica nel latte tramite apparecchiature automatiche operanti in citometria di flusso “produzione e validazione della conversione nazionale Bactoscan FC - anno 2012”. Report rev. 3 - 6 giugno 2013.

Losito F., Bottini G., De Ascentis A., Priolisi F.R., Mari A., Tarsitani G. and Antonini G., 2012. Qualitative and Quantitative Validation of the Micro Biological Survey Method for Listeria spp., Salmonella spp., Enterobacteriaceae and Staphylococcus aureus in Food Samples. American Journal of Food Technology, 7: 340-351. DOI: https://doi.org/10.3923/ajft.2012.340.351

Losito F. , Arienzo A. , Bottini G., Priolisi F.R., Mari A. and Antonini G. , 2014. Microbiological safety and quality of Mozzarella cheese assessed by the microbiological survey method. Journal of Dairy Science, 97:46-55. DOI: https://doi.org/10.3168/jds.2013-7026

Shultz, B.E. and S.I. Chan, 2001. Structures and proton-pumping strategies of mitochondrial respiratory enzymes. Annu. Rev. Biophys. Biomol. Struct., 30: 23-65. DOI: https://doi.org/10.1146/annurev.biophys.30.1.23

Slater, E.C., 2003. Keilin, cytochrome and the respiratory chain. J. Biol. Chem., 278: 16455-16461. DOI: https://doi.org/10.1074/jbc.X200011200

Traversetti L., Losito F., Arienzo A., Stalio O., Antonini G, Scalici M., 2017. Integrating running water monitoring tools with the Micro Biological Survey (MBS) method to improve water quality assessment. PLoS ONE, 12(9): e0185156. DOI: https://doi.org/10.1371/journal.pone.0185156

Published
2020-11-04
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Original Articles
Keywords:
Plate count at 30°C (per ml), MBS test, Flow citometry, Cow raw milk, Bactoscan FC
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How to Cite
1.
Cornacchia A, Saletti MA, Di Marzio V, Salini R, Marfoglia C, Tieri E, D’Alterio N, Marri N, Losito F, Arienzo A, Murgia L, Antonini G, Amatiste S, Leboffe L, Pomilio F. Application of the Micro Biological Survey analytical method for the determination of bacterial load in cow raw milk. Ital J Food Safety [Internet]. 2020Nov.4 [cited 2021Oct.16];9(3). Available from: https://pagepressjournals.org/index.php/ijfs/article/view/7696