https://doi.org/10.4081/ijfs.2022.10370
Prevalence of Brucella spp. in milk from aborted and non-aborted animals in Dhamar governorate, Yemen
Submitted: 19 January 2022
Accepted: 29 October 2022
Published: 5 December 2022
Accepted: 29 October 2022
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
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Brucella infection in animals is considered a great problem in most countries of the world. Our study designed to determine the prevalence of brucella in field animal’s milk in Dhamar governorate, Yemen. Total of 808 raw milk samples from non-aborted field animals, 120 milk samples from aborted animals, and 30 pasteurized milk samples were teste by Milk-Ring Test (MRT), milk-ELISA test, isolation and identification of brucella species, and antibiotic susceptibility. The prevalence of brucella in milk samples from field animals was 0.8%, 2.6%, and 2% in cows, sheep, and goat milk samples respectively with MRT, and 0.8%, 1.3% and 1.6% in cows, sheep and goat milk samples respectively with the milk- ELISA test. The prevalence rate in milk samples from aborted animals was 33%, 64% and 41.2% with the MRT and 39%, 49%, and 41.2% in cows, sheep and goats respectively with the milk-ELISA test. All pasteurized milk samples were negative for the milk-ELISA test. The result of isolation showed 0.1% of Brucella in milk samples from field animals while 9.2% from aborted animals. All isolates of Brucella species were sensitivities to rifampicin, doxycycline, kanamycin, gentamicin, streptomycin, tetracycline, and ciprofloxacin, while resistant to ampicillin, erythromycin, and novobiocin. In conclusion, the high prevalence of milk brucella especially in aborted animals needs focusing and build controlling strategies plans to decrease the losses to the economy and avoid transferred to humans with unpasteurized milk consumption.
Aggad H, Boukraa L, 2006. Prevalence of bovine and human brucella in western Algeria: comparison of screening tests. East Mediterr Health J 12:119–128.
Al Dahouk S, Nöckler K, Tomaso H, Splettstoesser WD, Jungersen G, Riber U, Petry T, Hoffmann D, Scholz HC, Hensel A, Neubauer H, 2005. Seroprevalence of brucella, tularemia, and yersiniosis in wild boars (Sus scrofa) from North-Eastern Germany. J Vet Med B 52:444–455.
DOI: https://doi.org/10.1111/j.1439-0450.2005.00898.x
Aldomy FMM, Jahans KL, Altarazi YH, 1992. Isolation of Brucella melitensis from aborting ruminants in Jordan. J Comp Pathol 107:239–242.
DOI: https://doi.org/10.1016/0021-9975(92)90040-2
Ali SAA, 1998. Bacterial and serological study on brucella in camels in Iraq - M.sc. Thesis, Faculty of Veterinary Medicine - Baghdad University.
Aliyev J, Alakbarova M, Garayusifova A, Omarov A, Aliyeva S, Fretin D, Godfroid J., 2022. Identification and molecular characterization of Brucella abortus and Brucella melitensis isolated from milk in cattle in Azerbaijan. BMC Vet Res 18:1-9.
DOI: https://doi.org/10.1186/s12917-022-03155-1
Al-Shamahy HA, Whitty CJ, Wright SG, 2000. Risk factors for human brucella in Yemen: a case control study. Epidemiol Infect 125:309–313.
DOI: https://doi.org/10.1017/S0950268899004458
Alton G., 1990. Brucella melitensis. “Animal brucella”. (K. Nielsen and J.R.Duncan, eds.). CRC Press Inc. Boston: 383-409.
Alton GG, Jones LM, Perrz DF, 1975. Laboratory techniques in brucellosis 2nd.ed. World Health Organization, Monograph Series 55, Geneva, Switzerland.
Alton GG, Jones LM, Angus RD, Verger JM, 1988. Techniques for the brucellosis laboratory. INRA, Paris.
Bauer AW, Kirby WM, Sherris JC, Turk M, 1966. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Path 45: 493-496.
DOI: https://doi.org/10.1093/ajcp/45.4_ts.493
Béjaoui A, Ben Abdallah I, Maaroufi A, 2022. Brucella spp. Contamination in Artisanal Unpasteurized Dairy Products: An Emerging Foodborne Threat in Tunisia. Foods 11:2269.
DOI: https://doi.org/10.3390/foods11152269
Bercovich Z, Moerman A, 1979. Non-specific positive Milk-Ring Test (s) in tank milk and Estrumater in the treatment of cattle. Tijdschrift voor Diergeneeskunde 104:713-716.
Biancifiori F, Nannini D, Di Matteo A, Belfiore P, 1996. Assessment of an indirect ELISA in milk for the diagnosis of ovine brucellosis. Comp Iramun Microbiol Infect Dis 19: 17-24.
DOI: https://doi.org/10.1016/0147-9571(95)00024-0
Bodur H, Erbay A, Akinci E, Aylin C, Mustafa AC, Balaban N, 2003. Neurobrucellosis in an endemic area of brucellosis. Scand J Infect Dis. 35:94–97.
DOI: https://doi.org/10.1080/0036554021000027000
Chand P, Rajpurohit AK, Malhotra AK, Poonia JS, 2005. Comparison of milk-ELISA and serum-ELISA for the diagnosis of Brucella melitensis infection in sheep milk. Vet Microbiol 108:305–311.
DOI: https://doi.org/10.1016/j.vetmic.2005.04.006
Commonwealth Department of Health National Biological Standard Laboratory, 1984. Standard for brucella abortus Milk-Ring Test antigen, Therapeutic Goods Act 1966, Order No. 10. Canberra. Australian.
Corbel M. J., 2006. Food and Agriculture Organization of the United Nations, World Health organization, World Organization for Animal Health. Brucellosis in humans and animals. Geneva: World Health Organization.
Corbel M.J. 1989. Microbiology of the genus of the brucella. (eds. E. Young and M. Corbel). In Brucellosis: Clinical and laboratory aspects (eds. E. Young and M. Corbel). CRC Press, Boca Raton, Fl:53-67.
DOI: https://doi.org/10.1201/9781003068518-7
Corbel M.J. 2006. Joint WHO -FAO-OIE. Brucellosis in humans and animals. WHO/CDS/EPR/2006.7.
Cowan S.T. 1993. Cowan and Steel’s Manual for the Identification of Medical bacteria G I Barrow and R K A Feltham, (des). 4th ed. Cambridge University press, Cambridge. USA, pp. 136-137.
European Commission. 2001. Brucellosis in sheep and goats (Brucella melitensis). Report of the Scientific Committee on Animal Health and Welfare of the European Commission.
FAO, 2003. Guidelines for coordinated human and animal brucellosis surveillance. Rome, Italy, FAO. (Animal Production and Health Paper No 156).
FAO/WHO, 1986. Joint FAO/Who expert committee on brucellosis. 6th report, World Health Organization Tech. Rep., No. 740, WHO, Geneva, Switzerland
Ferreira Ana Cristina, Cardoso Regina, Dias Travassos Isabel, Mariano Isabel, Belo Ana, Preto Isabel Rolão, Manteigas António, Fonseca António Pina, Corrêa Maria Inácia De Sá. 2003. Evaluation of a modified Rose Bengal test and an indirect Enzyme-Linked Immunosorbent Assay for the diagnosis of Brucella melitensis infection in sheep. Vet. Res. 34, 297–305.
DOI: https://doi.org/10.1051/vetres:2003005
General Administration of Livestock (GALR). 2007. Annual reports of the General Administration of Livestock during the period from 1974 – 2006 for the disease of endemic abortion. Ministry of Agriculture and Water Resources - Republic of Yemen.
Gupta VK, Verma DK, Rout PK, Singh SV, Vihan VS, 2006. Polymerase chain reaction (PCR) for detection of Brucella melitensis in goat milk. Small Rum Res 65 :79–84.
DOI: https://doi.org/10.1016/j.smallrumres.2005.05.024
Hadad JJ, Hammed DA, Alaboudi AR, 1997. Isolation of brucella strains from dairy products in Ninevah province, Iraq. Iraqi J Vet Sci 10:39-44.
Hamdy MER, Amin AS, 2002. Detection of Brucella species in the milk of infected cattle, sheep, goats and camels by PCR. Vet J 163:299-305.
DOI: https://doi.org/10.1053/tvjl.2001.0681
Hobbs IF, 1985. Comparison of the indirect enzyme Linked Immuno-sorbent Assays (ELISA) with the Complement fixation test (CFT) for the serodiagnosis of bovine brucellosis. NZ Vet J 33:112-116.
DOI: https://doi.org/10.1080/00480169.1985.35188
Hosie BD, Al-Bakri OM, Futter RJ, 1985. Survey of Brucellosis in goats and sheep in the Yemen Arab Republic: Comparison of tests for Brucella melitensis infection in sheep. Trop Anim Health Prod 17:93-9.
DOI: https://doi.org/10.1007/BF02360780
Hunter D, Allen J, 1972. An evaluation of milk and blood tests used to diagnose brucellosis. Vet. Rec. 91: 310.
DOI: https://doi.org/10.1136/vr.91.13.310
Islam M, Khatun M, Saha S, Basir MD, Hasan MM, 2018. Molecular detection of Brucella spp. from milk of seronegative cows from some selected area in Bangladesh. J Pathogens, 2018.
DOI: https://doi.org/10.1155/2018/9378976
Jalali A, Hemmatzadeh F, Momtaz H, Nilsson E, 2003. Elisa as an alternative method for the diagnosis of brucellosis in sheep. the 56th Brucellosis Research Conference. Sept. 15-17. University of Navarra, Pamplona (Spain).
Kang'ethe EK, Arimi SM, Omore AO, McDermott JJ, Nduhiu JG, Macharia JK, Githua A, 2000. The Prevalence of antibo-dies to brucella abortus in marketed milk in Kenya and its public health implications. Paper prepared for oral presentation at the 3PrdP all Africa Conference on Animal Agriculture. 6 – 9 november 2000.
Kerby PJ, Quiroga JL, McGrane JJ, Stagg DD, 1997. Field evaluation of an indirect ELISA for detection of brucellosis in lowland Bolivia. Trop Anim Hlth Prod 29:65-72.
DOI: https://doi.org/10.1007/BF02632320
Kerkhofs P, Botton Y, Thiange P, Dekeyser P, Limetb JN, 1990. Diagnosis of bovine brucellosis by Enzyme immunoassay of milk. Vet Microbiol 24:73-80.
DOI: https://doi.org/10.1016/0378-1135(90)90052-W
Kumar VN, Bharathi MV, Porteen K, 2017. Risk factors associated with prevalence of bovine brucellosis in milk from Tamil Nadu, India. Int J Curr Microbiol Appl Sci 6:2604-9.
DOI: https://doi.org/10.20546/ijcmas.2017.607.367
Mubarak A, 1996. Animal diseases and prevention and control measures - Omar Al-Mukhtar University - Libya.
Negrón ME, Kharod GA, Bower WA, Walke H, 2019. Notes from the field: human Brucella abortus RB51 infections caused by consumption of unpasteurized domestic dairy products - United States, 2017–2019. Morbidity and Mortality Weekly Report 68:185.
DOI: https://doi.org/10.15585/mmwr.mm6807a6
Neilsen K, Smith P, Gall D, Perez B, Cosma C, Muller P, Trottier J, Cote G, Boag L, Bosse J, 1996. Development and validation of an indirect enzyme immunoassay for detection of antibody to Brucela arbotus in milk. Vet Microbiol 52:165-73.
DOI: https://doi.org/10.1016/0378-1135(96)00059-4
Nichols P, 1991. Social survey methods: a field guide for development workers.No.6. series editor Brian Pratt. Oxfam, Oxford.
Nicoletti P, 1969. Further evaluation of serologic tests procedures used to diagnose brucellosis. Am J Vet Res 30: 1811.
Ning P, Guo M, Guo K, Xu L, Ren M, Cheng Y, Zhang Y, 2013. Identification and effect decomposition of risk factors for Brucella contamination of raw whole milk in China. PLoS One 8e68230.
DOI: https://doi.org/10.1371/journal.pone.0068230
Nofal AS, El-Leboudy AA, El-Makarem HSA, El-Rahman AHA, 2017. Prevalence of Brucella Organism in Milk and Serum Samples of Some Lactating Dairy Animals. Alexandria J Vet Sci 55.
DOI: https://doi.org/10.5455/ajvs.278394
OIE, 2002. OIE Animal Health Code. Also available online at: www.oie.int.
OIE, 2004. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. 5th Ed. Paris, France.
Otlu S, Sahin M, Atabay HI, Unver A, 2008. Serological Investigations of Brucellosis in Cattle, Farmers and Veterinarians in the Kars District of Turkey. Acta Vet Brno 77:117–21.
DOI: https://doi.org/10.2754/avb200877010117
Pfeiffer DU, 2002. Veterinary Epidemiology – An Introduction. Epidemiology division department of veterinary clinical sciences, The Royal Veterinary College, University of London UK.
Poester FP, Samartino LE, Santos RL, 2013. Pathogenesis and pathobiology of brucellosis in livestock. Rev Sci Tech 32:105–15.
DOI: https://doi.org/10.20506/rst.32.1.2193
Radwan AI, Bekairi SI, Mukayel AA, Al-Bokmy AM, Prasad PV, Azar FN, Coloyan ER, 1995. Control of Brucella melitensis infection in a large camel herd in Saudi Arabia using antibiotherapy and vaccination with Rev.1 vaccine. Rev Sci Tech 14:719–32.
DOI: https://doi.org/10.20506/rst.14.3.860
Refai M, 2002. incidence and control of brucellosis in the near east region. Vet Microbiol, 90:81-110.
DOI: https://doi.org/10.1016/S0378-1135(02)00248-1
Sting R, Ortmann G, 2000. Experience with a simple ELISA for immunodiagnosis of Brucella in cattle, sheep and goats. Berliner Munchener Tierrztliche Wochenschrift 113:22-8.
Sutherland SS, Evans RJ, Bathgate J, 1986. Application of an enzyme linked immunosorbent assay in the final stages of a bovine brucellosis eradication program. Aust Vet J 63:412-5.
DOI: https://doi.org/10.1111/j.1751-0813.1986.tb15886.x
Sutra L, Caffin JP, Dubray G, 1986. Role of immuno-globulins in the Brucella Milk-Ring Test. Vet Microbiol 12:359–66.
DOI: https://doi.org/10.1016/0378-1135(86)90085-4
Turkmani A, Ioannidis A, Christidou A, Psaroulaki A, Loukaides F, Tselentis Y, 2006. In vitro susceptibilities of Brucella melitensis isolates to eleven antibiotics. Ann Clin Microbiol Antimicrob 2;5:24.
DOI: https://doi.org/10.1186/1476-0711-5-24
USDA and APHIS. 1998. Brucellosis Eradication: Uniform Methods and Rules. USDA-APHIS, Washington D.C.
Wainaina M, Aboge GO, Omwenga I, Ngaywa C, Ngwili N, Kiara H, ... Bett B, 2020. Detection of Brucella spp. in raw milk from various livestock species raised under pastoral production systems in Isiolo and Marsabit counties, northern Kenya. Trop Anim Health Prod 52:3537-44.
DOI: https://doi.org/10.1007/s11250-020-02389-1
Wasseif SM, 1992. Brucellosis in Sharkia governorate – an epidemiological study. Egypt J Occup Med 10: 247-58.
WHO, 2005. Brucellosis in humans and animals. WHO guidance, Geneva.
Yamazhan T, Aydemir S, Tünger A, Serter D, Gökengin D, 2005. In vitro Activities of Various Antimicrobials against Brucella melitensis Strains in the Aegean Region in Turkey. Med Princ Pract 14:413–6.
DOI: https://doi.org/10.1159/000088122
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Al-Afifi AH, Alewy Almashhadany D, Al-Azazi AS, Khalaf AM, Naji Ahmed Odhah M, Al-Gabri NA. Prevalence of <em>Brucella</em> spp. in milk from aborted and non-aborted animals in Dhamar governorate, Yemen. Ital J Food Safety [Internet]. 2022 Dec. 5 [cited 2024 Dec. 21];11(4). Available from: https://www.pagepressjournals.org/ijfs/article/view/10370
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