https://doi.org/10.4081/aiua.2020.4.340
Protective effect of cordycepin on experimental renal ischemia/reperfusion injury in rats
Submitted: October 19, 2020
Accepted: October 27, 2020
Published: December 18, 2020
Accepted: October 27, 2020
Abstract Views: 1093
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
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Aim: To date, various molecules have been investigated to reduce the effect of renal ischemia/reperfusion (I/R) injury. However, none have yet led to clinical use. The present study aimed to investigate the protective effect of cordycepin (C) on renal I/R injury in an experimental rat model. Materials and methods: Twenty-four mature Sprague Dawley female rat was randomly divided into three groups: Sham, I/R, I/R+C. All animals underwent abdominal exploration. To induce I/R injury, an atraumatic vascular bulldog clamp was applied to the right renal pedicle for 60 minutes (ischemia) and later clamp was removed to allow reperfusion in all rats, except for the sham group. In the I/R + C group, 10 mg/kg C was administered intraperitoneally, immediately after reperfusion. After 4 hours of reperfusion, the experiment was terminated with right nephrectomy. Histological studies and biochemical analyses were performed on the right nephrectomy specimens. EGTI (endothelial, glomerular, tubulointerstitial) histopathology scoring and semi-quantitative analysis of renal cortical necrosis were used for histological analyses and superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), total oxidant status (TOS) for biochemical analyses. Results: Histopathological examination of the tissue damage revealed that all kidneys in the sham group were normal. The I/R group had higher histopathological scores than the I/R + C group. In the biochemical analysis of the tissues, SOD, MDA, TOS values were found to be statistically different in the I/R group compared to the I/R + C group (p: 0.004, 0.004, 0.001 respectively). Conclusions: Intraperitoneal cordycepin injection following ischemia preserve renal tissue against oxidative stress in a rat model of renal I/R injury.
Zuk A, Bonventre JV. Acute kidney injury. Annual review of medicine. 2016;67:293-307.
DOI: https://doi.org/10.1146/annurev-med-050214-013407
Wang L, Liu X, Chen H, Chen Z, Weng X, Qiu T, et al. Effect of picroside II on apoptosis induced by renal ischemia/reperfusion injury in rats. Experimental and therapeutic medicine. 2015;9(3):817-22.
DOI: https://doi.org/10.3892/etm.2015.2192
Zhang J, Zou Yr, Zhong X, Deng HDF, Pu L, Peng K, et al. Erythropoietin pretreatment ameliorates renal ischaemia‐reperfusion injury by activating PI3K/Akt signalling. Nephrology. 2015;20(4):266-72.
DOI: https://doi.org/10.1111/nep.12384
Malek M, Nematbakhsh M. Renal ischemia/reperfusion injury; from pathophysiology to treatment. Journal of renal injury prevention. 2015;4(2):20.
Yue K, Ye M, Zhou Z, Sun W, Lin X. The genus C ordyceps: a chemical and pharmacological review. Journal of Pharmacy and Pharmacology. 2013;65(4):474-93.
DOI: https://doi.org/10.1111/j.2042-7158.2012.01601.x
Nakamura K, Shinozuka K, Yoshikawa N. Anticancer and antimetastatic effects of cordycepin, an active component of Cordyceps sinensis. Journal of pharmacological sciences. 2015;127(1):53-6.
DOI: https://doi.org/10.1016/j.jphs.2014.09.001
Okur MH, Arslan S, Aydogdu B, Zeytun H, Basuguy E, Arslan MS, et al. Protective effect of cordycepin on experimental testicular ischemia/reperfusion injury in rats. Journal of Investigative Surgery. 2018;31(1):1-8.
DOI: https://doi.org/10.1080/08941939.2016.1246629
Toprak T, Sekerci CA, Aydın HR, Ramazanoglu MA, Arslan FD, Basok BI, et al. Protective effect of chlorogenic acid on renal ischemia/reperfusion injury in rats. Archivio Italiano di Urologia e Andrologia. 2020;92(2).
DOI: https://doi.org/10.4081/aiua.2020.2.153
Medeiros PJd, Villarim Neto A, Lima FP, Azevedo ÍM, Leão LRdS, Medeiros AC. Effect of sildenafil in renal ischemia/reperfusion injury in rats. Acta cirurgica brasileira. 2010;25(6):490-5.
DOI: https://doi.org/10.1590/S0102-86502010000600006
Chavez R, Fraser DJ, Bowen T, Jenkins RH, Nesargikar P, Pino-Chavez G, et al. Kidney ischaemia reperfusion injury in the rat: the EGTI scoring system as a valid and reliable tool for histological assessment. Journal of Histology and Histopathology. 2016;3.
DOI: https://doi.org/10.7243/2055-091X-3-1
Uchiyama M, Mihara M. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Analytical biochemistry. 1978;86(1):271-8.
DOI: https://doi.org/10.1016/0003-2697(78)90342-1
Erel O. A new automated colorimetric method for measuring total oxidant status. Clinical biochemistry. 2005;38(12):1103-11.
DOI: https://doi.org/10.1016/j.clinbiochem.2005.08.008
Goth L. A simple method for determination of serum catalase activity and revision of reference range. Clinica chimica acta. 1991;196(2-3):143-51.
DOI: https://doi.org/10.1016/0009-8981(91)90067-M
Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clinical chemistry. 1988;34(3):497-500.
DOI: https://doi.org/10.1093/clinchem/34.3.497
Aksu U, Demirci C, Ince C. The pathogenesis of acute kidney injury and the toxic triangle of oxygen, reactive oxygen species and nitric oxide. Controversies in Acute Kidney Injury. 174: Karger Publishers; 2011. p. 119-28.
DOI: https://doi.org/10.1159/000329249
Salvadori M, Rosso G, Bertoni E. Update on ischemia-reperfusion injury in kidney transplantation: Pathogenesis and treatment. World journal of transplantation. 2015;5(2):52.
DOI: https://doi.org/10.5500/wjt.v5.i2.52
Ferenbach DA, Bonventre JV. Mechanisms of maladaptive repair after AKI leading to accelerated kidney ageing and CKD. Nature Reviews Nephrology. 2015;11(5):264-76.
DOI: https://doi.org/10.1038/nrneph.2015.3
Molitoris BA. Therapeutic translation in acute kidney injury: the epithelial/endothelial axis. The Journal of clinical investigation. 2014;124(6):2355-63.
DOI: https://doi.org/10.1172/JCI72269
Basile DP, Donohoe D, Roethe K, Osborn JL. Renal ischemic injury results in permanent damage to peritubular capillaries and influences long-term function. American Journal of Physiology-Renal Physiology. 2001.
DOI: https://doi.org/10.1152/ajprenal.2001.281.5.F887
Yang N, Luo M, Li R, Huang Y, Zhang R, Wu Q, et al. Blockage of JAK/STAT signalling attenuates renal ischaemia-reperfusion injury in rats. Nephrology Dialysis Transplantation. 2008;23(1):91-100.
DOI: https://doi.org/10.1093/ndt/gfm509
Si Y, Bao H, Han L, Shi H, Zhang Y, Xu L, et al. Dexmedetomidine protects against renal ischemia and reperfusion injury by inhibiting the JAK/STAT signaling activation. Journal of translational medicine. 2013;11(1):141.
DOI: https://doi.org/10.1186/1479-5876-11-141
Yeboah M, Xue X, Duan B, Ochani M, Tracey K, Susin M, et al. Cholinergic agonists attenuate renal ischemia–reperfusion injury in rats. Kidney international. 2008;74(1):62-9.
DOI: https://doi.org/10.1038/ki.2008.94
Chu C, He W, Kuang Y, Ren K, Gou X. Celastrol protects kidney against ischemia–reperfusion-induced injury in rats. Journal of Surgical Research. 2014;186(1):398-407.
DOI: https://doi.org/10.1016/j.jss.2013.07.048
Hwang HS, Yang KJ, Park KC, Choi HS, Kim SH, Hong SY, et al. Pretreatment with paricalcitol attenuates inflammation in ischemia–reperfusion injury via the up-regulation of cyclooxygenase-2 and prostaglandin E2. Nephrology Dialysis Transplantation. 2013;28(5):1156-66.
DOI: https://doi.org/10.1093/ndt/gfs540
Johnson KJ, Weinberg JM. Postischemic renal injury due to oxygen radicals. Current opinion in nephrology and hypertension. 1993;2(4):625-35.
DOI: https://doi.org/10.1097/00041552-199307000-00014
Paller MS. The cell biology of reperfusion injury in the kidney. J Investig Med. 1994;42:632-9.
Bonventre JV. Mechanisms of ischemic acute renal failure. Kidney international. 1993;43(5):1160-78.
DOI: https://doi.org/10.1038/ki.1993.163
Sugiyama S, Hanaki Y, Ogawa T, Hieda N, Taki K, Ozawa T. The effects of SUN 1165, a novel sodium channel blocker, on ischemia-induced mitochondrial dysfunction and leakage of lysosomal enzymes in canine hearts. Biochemical and biophysical research communications. 1988;157(2):433-9.
DOI: https://doi.org/10.1016/S0006-291X(88)80267-5
Ramesh T, Yoo S-K, Kim S-W, Hwang S-Y, Sohn S-H, Kim I-W, et al. Cordycepin (3′-deoxyadenosine) attenuates age-related oxidative stress and ameliorates antioxidant capacity in rats. Experimental gerontology. 2012;47(12):979-87.
DOI: https://doi.org/10.1016/j.exger.2012.09.003
Won K-J, Lee S-C, Lee C-K, Lee HM, Lee SH, Fang Z, et al. Cordycepin attenuates neointimal formation by inhibiting reactive oxygen species–mediated responses in vascular smooth muscle cells in rats. Journal of pharmacological sciences. 2009;109(3):403-12.
DOI: https://doi.org/10.1254/jphs.08308FP
Li L, He D, Yang J, Wang X. Cordycepin inhibits renal interstitial myofibroblast activation probably by inducing hepatocyte growth factor expression. Journal of pharmacological sciences. 2011:1111300626-.
DOI: https://doi.org/10.1254/jphs.11127FP
Cheng Z, He W, Zhou X, Lv Q, Xu X, Yang S, et al. Cordycepin protects against cerebral ischemia/reperfusion injury in vivo and in vitro. European journal of pharmacology. 2011;664(1-3):20-8.
DOI: https://doi.org/10.1016/j.ejphar.2011.04.052
Han F, Dou M, Wang Y, Xu C, Li Y, Ding X, et al. Cordycepin protects renal ischemia/reperfusion injury through regulating inflammation, apoptosis, and oxidative stress. Acta Biochimica et Biophysica Sinica. 2020;52(2):125-32.
DOI: https://doi.org/10.1093/abbs/gmz145
Supporting Agencies
University of Health Sciences, Turkey (BAP)How to Cite
Aydin, H. R., Sekerci, C. A., Yigit, E., Kucuk, H., Kocakgol, H., Kartal, S., Tanidir, Y., & Deger, O. (2020). Protective effect of cordycepin on experimental renal ischemia/reperfusion injury in rats. Archivio Italiano Di Urologia E Andrologia, 92(4). https://doi.org/10.4081/aiua.2020.4.340
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