https://doi.org/10.4081/aiua.2021.2.148

Gas6 expression and Tyrosine kinase Axl Sky receptors: Their relation with tumor stage and grade in patients with bladder cancer

Vol. 93 No. 2 (2021)
Submitted: March 31, 2021
Accepted: May 17, 2021
Published: June 28, 2021
Bladder cancer, Gas6, Axl, Sky, Tyro3
Abstract Views: 1325
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Authors

Murat Akgül
drmuratakgul@gmail.com
Department of Urology, Tekirdag Namık Kemal University Medical School, Tekirdag, Turkey.
Özgür Baykan
Department of Biochemistry, Balıkesir University Medical School, Balıkesir, Turkey.
Zeynep Çağman
Department of Biochemistry, Bezmialem University, School of Pharmacy, Istanbul, Turkey.
Mustafa Özyürek
Department of Physiology, Marmara University, School of Medicine, Istanbul, Turkey.
İlker Tinay
Anadolu Medical Center, Gebze, Kocaeli, Turkey.
Cem Akbal
Department of Urology, Acıbadem University, School of Medicine, Istanbul, Turkey.
Fikriye Uras
Department of Biochemistry, Marmara University, School of Pharmacy, Istanbul, Turkey.
Levent Türkeri
Department of Urology, Acıbadem University, School of Medicine, Istanbul, Turkey.

Objectives: It has been shown that the dysregulation of tyrosine kinase Axl receptor and its ligand growth arrest-specific gene (Gas6) are associated with poor prognosis in various types of tumors but there is not enough study about their importance in bladder cancer (BC). We evaluated the relation of Gas6 gene expression and tyrosine- kinase Axl and Sky (Tyro 3) receptors with tumor stage and grade in patients with BC.
Material and Methods: The study group consists of 55 patients whose transurethral resection of bladder (TUR-B) has been performed due to BC and the control group consists of 12 patients with normal bladder mucosa. In tissues mRNAs of Gas6, Axl, and Sky receptors were examined by quantitative (Real-Time) PCR (qPCR). Protein expression was measured by immunohistochemistry. Plasma Gas6 protein levels were compared with control group by ELISA method.
Results: Patients with BC were grouped as Ta low (n=17), Ta high (n=5), T1 low (n=9), T1 high (n=8) and T2 (n=16) according to their TUR-B pathologies. The qPCR analysis showed that the expression of Gas6 gene and Axl receptor is higher in the tumor-positive group and the immune-histochemical showed that the bladder samples of the tumor-positive group stained significantly positive. When the patients are grouped according to the TUR-B pathologies, a statistical significant difference was observed among groups in the qPCR analysis ratios of Gas6 gene and Axl receptor by (p < 0.05) but no significance was found for Sky receptor (p > 0.05). When Gas6 protein levels in plasma samples were compared by ELISA method, a statistical significance was determined among groups (p = 0.001).
Conclusions: Our findings indicate that mRNAs of Gas6 and Axl receptor are closely related to tumor stage and grade in patients with BC. Further studies are needed for understanding the role of Gas6 and its receptors on the neoplastic transformation in terms of novel biomarkers and potential therapeutic targets.

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Supporting Agencies

This investigation was supported by Marmara University Scientific Research Projects Committee.

How to Cite

Akgül, M., Baykan, Özgür, Çağman , Z., Özyürek , M. ., Tinay, İlker, Akbal , C. ., Uras , F. ., & Türkeri , L. . (2021). Gas6 expression and Tyrosine kinase Axl Sky receptors: Their relation with tumor stage and grade in patients with bladder cancer. Archivio Italiano Di Urologia E Andrologia, 93(2), 148–152. https://doi.org/10.4081/aiua.2021.2.148

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