https://doi.org/10.4081/aiua.2022.3.265

One step nucleic acid amplification (OSNA) for detection of lymph node metastasis during robotic radical prostatectomy for prostate cancer: A pilot study

Vol. 94 No. 3 (2022)
Submitted: January 24, 2022
Accepted: March 2, 2022
Published: September 26, 2022
Prostate cancer; Metastases; Lymph nodes; One step nucleic acid amplification.
Abstract Views: 1470
pdf: 447
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Authors

Omer Burak Argun
Acibadem Mehmet Ali Aydinlar University, School of Medicine, Department of Urology, Istanbul, Turkey.
Panagiotis Mourmouris
thodoros13@yahoo.com
National and Kapodistrian University of Athens, Sismanogleio General Hospital, 2nd Department of Urology, Athen, Greece.
Yesim Saglican
Acibadem Mehmet Ali Aydinlar University, School of Medicine, Department of Pathology, Istanbul, Turkey.
Tunkut Doganca
Acibadem Taksim Hospital, Department of Urology, Istanbul, Turkey.
Mustafa Bilal Tuna
https://orcid.org/0000-0003-2845-9801
Acibadem Maslak Hospital, Department of Urology, Istanbul, Turkey.
Cavit Kerem Kayhan
Acibadem Maslak Hospital, Department of Pathology, Istanbul, Turkey.
Ozge Yalcinkaya
Acibadem Maslak Hospital, Department of Pathology, Istanbul, Turkey.
Ilter Tufek
Acibadem Mehmet Ali Aydinlar University, School of Medicine, Department of Urology, Istanbul, Turkey.
Halil Kara
Acibadem Maslak Hospital, Department of General Surgery, Istanbul, Turkey.
Can Obek
Acibadem Mehmet Ali Aydinlar University, School of Medicine, Department of Urology, Istanbul, Turkey.
Umit Ince
Acibadem Mehmet Ali Aydinlar University, School of Medicine, Department of Pathology, Istanbul, Turkey.
Lazaros Tzelves
https://orcid.org/0000-0003-4619-9783
National and Kapodistrian University of Athens, Sismanogleio General Hospital, 2nd Department of Urology, Athens, Greece.
Andreas Skolarikos
National and Kapodistrian University of Athens, Sismanogleio General Hospital, 2nd Department of Urology, Athens, Greece.
Ali Riza Kural
Acibadem Mehmet Ali Aydinlar University, School of Medicine, Department of Urology, Istanbul, Turkey.

Introduction: The OSNA technique is based on reverse transcription loop-mediated DNA amplification for the detection of cytokeratin 19 (CK19) messen-ger RNA (mRNA). The purpose of our paper, which represents the first study in the literature, is to test the accuracy of this method in the detection of lymph node metastases in patients undergoing robotic radical prostatectomy with lymph node dis-section.
Methods: Our cohort consisted of patients that have undergone robotic radical prostatectomy with extended lymph node dissec-tion. Lymph nodes were evaluated with imprint technique and then with frozen section examination. The remaining tissue was evaluated by OSNA method. Lymph nodes were defined as ‘neg-ative’ or ‘positive’ according to mRNA copy number.
Results: 7 patients and 25 lymph nodes were included in our cohort. Two patients were found negative with all pathology methods. In one patient the standard stains revealed a suspi-cious outcome but it was positive for micrometastasis with OSNA. In another patient the outcome was positive for standard stains and negative for OSNA. Finally, 2 patients were found positive for OSNA and negative for imprint methods. Conclusions: One Step Nucleic Acid Amplification (OSNA) method using CK19 seems to fail in detection of lymph node metastases in prostate cancer patients undergoing radical prostatectomy and lymph node dissection.

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How to Cite

Argun, O. B., Mourmouris, P., Saglican, Y., Doganca, T., Tuna, M. B., Kayhan, C. K., Yalcinkaya, O., Tufek, I., Kara, H., Obek, C., Ince, U., Tzelves, L., Skolarikos, A., & Kural, A. R. (2022). One step nucleic acid amplification (OSNA) for detection of lymph node metastasis during robotic radical prostatectomy for prostate cancer: A pilot study. Archivio Italiano Di Urologia E Andrologia, 94(3), 265–269. https://doi.org/10.4081/aiua.2022.3.265

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