https://doi.org/10.4081/ijfs.2009.1399

SURVEY ON V. CHOLERAE, V. VULNIFICUS AND V. PARAHAEMOLYTICUS IN BIVALVE MOLLUSCS OF THE ADRIATIC SEA AND PROPOSAL OF AN ANALYTICAL PROTOCOL

AIVI - June 2009 - Issue 4
Submitted: 21 February 2013
Accepted: 21 February 2013
Published: 21 June 2009
Bivalve molluscs, Adriatic sea, V. parahaemolyticus, V. cholerae, V. vulnificus
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Authors

P. Serratore
lucia.zoppi@pagepress.org
Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale Università di Bologna, .
A. Piano
Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale Università di Bologna, .
S. Piraccini
Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale Università di Bologna, .
M. Trentini
Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale Università di Bologna, .
E. Zavatta
Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale Università di Bologna, .
M. Grodzki
Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale Università di Bologna, .
M.L. Valeri
Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale Università di Bologna, .
Bivalve molluscs from Adriatic sea were analyzed for V. parahaemolyticus, V. cholerae e V. vulnificus presence. The isolates on TCBS Agar and m-CPC Agar were selected on the basis of a new biochemical screening, that showed a good performance, because among 2344 strains from primary culture only 237 (10%) were presumptively assigned to the species of interest. The PCR analyses was performed for the target genes toxR hlyA, ctxA, tcpI (V. cholerae), toxR, tl, tdh, trh (V. parahaemolyticus), vvhA and viuB (V. vulnificus). Among the 9 strains confirmed to belong to V. parahaemolyticus specie, 6 were sucrose positive. On 215 samples of molluscs only 5 resulted positive for V. parahaemolyticus being toxR+, tl+, although non pathogenic (tdh-, trh-), and none for V. cholerae e V. vulnificus.

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1.
Serratore P, Piano A, Piraccini S, Trentini M, Zavatta E, Grodzki M, Valeri M. SURVEY ON V. CHOLERAE, V. VULNIFICUS AND V. PARAHAEMOLYTICUS IN BIVALVE MOLLUSCS OF THE ADRIATIC SEA AND PROPOSAL OF AN ANALYTICAL PROTOCOL. Ital J Food Safety [Internet]. 2009 Jun. 21 [cited 2024 Nov. 5];1(4):33-8. Available from: https://www.pagepressjournals.org/ijfs/article/view/ijfs.2008.4.33

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