https://doi.org/10.4081/ijfs.2017.6904

Evaluation of bovine beta casein polymorphism in two dairy farms located in northern Italy

Vol. 6 No. 3 (2017)
Submitted: 13 July 2017
Accepted: 28 August 2017
Published: 29 September 2017
Dairy cows, Beta casein, Allelic variants, Sequencing
Abstract Views: 2561
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Authors

Elisa Massella
elisa.massella3@unibo.it
Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia (BO), Italy.
Silvia Piva
Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia (BO), Italy.
Federica Giacometti
Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia (BO), Italy.
Gaetano Liuzzo
A.U.S.L of Modena Carpi district, Carpi (MO), Italy.
Angelo Vittorio Zambrini
Department of Quality, Innovation, Safety, Environment, Granarolo S.p.A., Bologna, Italy.
Andrea Serraino
Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell’Emilia (BO), Italy.
Bovine beta casein A1 is one of the most common variants in dairy cattle breeds; it is considered a risk factor in milk intolerance and in other important human diseases, because of the bioactive peptide beta casomorphin-7 (BCM7) produced by raw or processed A1-milk, but not by A2- milk, during digestion. The aim of this study was to perform a cheap and rapid method to investigate beta casein polymorphism in copious animals. The study included 2 dairy farms with a totally of 1230 cows. Beta casein genotypes were estimated evaluating Exon 7 region of bovine beta casein gene (CSN2) by sequences analysis. In the population included in the study 5 variants (A1, A2, B, F, I) and 13 genotypes (A1A1, A1A2, A1B, A1F, A1I, A2A2, A2B, A2F, A2I, BB, BF, BI, FI) were detected. The method showed high sensibility and specificity, resulted low-cost and few time consuming.

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How to Cite

1.
Massella E, Piva S, Giacometti F, Liuzzo G, Zambrini AV, Serraino A. Evaluation of bovine beta casein polymorphism in two dairy farms located in northern Italy. Ital J Food Safety [Internet]. 2017 Sep. 29 [cited 2024 Nov. 23];6(3). Available from: https://www.pagepressjournals.org/ijfs/article/view/6904

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