https://doi.org/10.4081/ijfs.2016.5652

Dissemination and persistence of Pseudomonas spp. in small-scale dairy farms

Vol 5, No 2 (2016)
Submitted: 29 November 2015
Accepted: 8 February 2016
Published: 2 May 2016
Pseudomonas, Small-scale dairy farms, Water, Typing, Persistence
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Authors

Daniele Michele Nucera
daniele.nucera@unito.it
http://orcid.org/0000-0002-0978-8346
Department of Agricultural, Forest and Food Sciences, University of Turin, Grugliasco (TO), Italy.
Sara Lomonaco
Department of Veterinary Sciences, University of Turin, Grugliasco (TO), Italy.
Patrizia Morra
Department of Veterinary Sciences, University of Turin, Grugliasco (TO), Italy.
Marco Francesco Ortoffi
Department of Veterinary Sciences, University of Turin, Grugliasco (TO), Italy.
Daniele Giaccone
Regional Association of Farmers of Piedmont, Turin, Italy.
Maria Ausilia Grassi
Department of Veterinary Sciences, University of Turin, Grugliasco (TO), Italy.
This study was aimed at collecting data on presence, dissemination and persistence of Pseudomonas in small-scale dairy farms. Six farms (located in Piedmont) were visited three times over 2014: 116 waters (wells and different faucets/pipes) and 117 environmental samples (milking equipments and drains) were collected. Enumeration of Pseudomonadaceae was performed, 3-5 colonies/samples were selected for identification via 16SrDNA/oprI polymerase chain reaction (PCR), and typed by enterobacterial-repetitive- intergenic-consensus (ERIC)-PCR. Pseudomonadaceae were detected in 77% of samples. No statistical differences were found among proportions of positives across farms, sample typologies and seasons. Most isolates were Pseudomonas fluorescens (45%), and ERIC-PCR showed 32 persistent types diffused across farms. All in all, Pseudomonas spp. represents a challenge, considering its presence over time in water as well as in teat cups, indicating a continuous source of contamination. Moreover, persistency of strains may indicate biofilm-formation and/or sanitisers resistance, therefore emphasising the role of primary production for preventing milk contamination by Pseudomonas spp.

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Regione Piemonte

How to Cite

1.
Nucera DM, Lomonaco S, Morra P, Ortoffi MF, Giaccone D, Grassi MA. Dissemination and persistence of Pseudomonas spp. in small-scale dairy farms. Ital J Food Safety [Internet]. 2016 May 2 [cited 2024 Nov. 23];5(2). Available from: https://www.pagepressjournals.org/ijfs/article/view/5652

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