https://doi.org/10.4081/ijfs.2024.12117
Development of a new method for extracting histamine from marine fish flesh using the salting-out technique
Submitted: 22 November 2023
Accepted: 2 February 2024
Published: 19 February 2024
Accepted: 2 February 2024
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
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A simple and practical method was developed to extract histamine from fish products using sodium chloride (NaCl). After obtaining a saline extract from fish samples, histamine was derivatized by a condensation reaction with ortho-phthalaldehyde. Fluorescence intensity was measured by a fluorimeter. The first part of this work concerned a solid-liquid extraction tested with samples from the food analysis performance assessment scheme. The best histamine extraction yield (97%) was obtained using an extraction time of 4 minutes, a temperature of 40°C, and a NaCl/water ratio of 41% (w/w). The second part focused on a liquid-liquid extraction carried out on standard solutions of histamine (45, 90, and 180 mg/kg). The use of NaCl (41%) and trichloroacetic acid [(TCA) 10%] did not show any significant difference in extraction yield. The yield obtained was 99.15-100.1% for TCA (10%) and 98.65-99.45% for NaCl (41%). The validation criteria (repeatability and reproducibility) were checked by evaluating the reliability of the method. Extraction using NaCl has proven to be an interesting alternative method for the extraction of histamine from fish, as it is reliable, inexpensive, and less hazardous.
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1.
Aoua C, Yacoubi B, Zekhnini A. Development of a new method for extracting histamine from marine fish flesh using the salting-out technique. Ital J Food Safety [Internet]. 2024 Feb. 19 [cited 2024 Dec. 22];13(1). Available from: https://www.pagepressjournals.org/ijfs/article/view/12117
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