https://doi.org/10.4081/ijfs.2024.11600

Insect-based products commercialized online: a snapshot of lipid oxidation and mineral content

Early Access
Submitted: 21 July 2023
Accepted: 23 May 2024
Published: 6 August 2024
Lipid oxidation, heavy metals, mineral elements, food safety, shelf-stable food
Abstract Views: 221
PDF: 92
SUPPLEMENTARY MATERIAL: 23
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Authors

Stefania Balzan
Department of Comparative Biomedicine and Food Science, University of Padova, Legnaro, Italy.
Luca Fasolato
Department of Comparative Biomedicine and Food Science, University of Padova, Legnaro, Italy.
Federico Fontana
Department of Comparative Biomedicine and Food Science, University of Padova, Legnaro, Italy.
Sarah Currò
sarah.curro@unipd.it
Department of Comparative Biomedicine and Food Science, University of Padova, Legnaro, Italy.
Enrico Novelli
Department of Comparative Biomedicine and Food Science, University of Padova, Legnaro, Italy.

This research aims to monitor the conservation status of the lipid and mineral contents of four shelf-stable insect-based products (yellow mealworm, house cricket, mole cricket, and silkworm) marketed online. A total of 32 single-species packs were purchased from various online commercial suppliers. Moisture, lipids, fatty acids, titratable acidity, mineral elements, and primary and secondary lipid oxidation products were determined. Statistical multivariate approaches were applied to investigate the contribution of each chemical variable to the characterization of edible insects. Titratable acidity (up to 37.3 g oleic acid per 100 g of crickets), as well as primary and secondary lipid oxidation products, showed great variability within and between species. The study revealed a significant occurrence of rancidity (45.5% of the samples exceeded the peroxide limit of 10 mEqO2/kg; 100% of the samples exceeded the indication of 1 mg/kg malondialdehyde), whereas the heavy metal content indicated a relatively safe condition, suggesting the absence of potential risks to human health. Both the chemical and the elemental properties could be regarded as potential characteristics suitable for authenticating this food matrix. This study contributes to the description of several chemical features in commercialized processed insect-based products, aiming to highlight possible safety concerns and identify those unfit for human consumption.

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Citations

Crossref
Scopus
Google Scholar
Europe PMC
Barcarolo R, Novelli E, Balzan S, Granata A, Antonetti P, 2007. PCB’s andhexachlorobenzene determination in fish by isotope dilution and GC-MS negative chemical ionisation mode. Conference presentation ate the 25th Informal meeting on mass spectrometry, 6th–10th May, 2007, Nyíregyháza-Sóstó, Hungary, Comm.MoPo30.
Bonoli M, Caboni MF, Rodriguez-Estrada MT, Lercker G, 2007. Effect of feeding fat sources on the quality and composition of lipids of precooked ready-to-eat fried chicken patties, Food Chem 101:1327-37. DOI: https://doi.org/10.1016/j.foodchem.2006.03.038
Botsoglou NA, Fletouris DJ, Papageorgiou GE, Vassilopoulos VN, Mantis AJ, Trakatellis AG, 1994. Rapid, sensitive, and specific thiobarbituric acid method for measuring lipid-peroxidation in animal tissue, food, and feedstuff samples. J Agric Food Chem 42:1931-7. DOI: https://doi.org/10.1021/jf00045a019
Chen X, Feng Y, Chen Z, 2009. Common edible insects and their utilization in China. Entomol Res 39:299-303. DOI: https://doi.org/10.1111/j.1748-5967.2009.00237.x
Codex Alimentarius, 1997. Report of the fifteenth session of the codex committee on fats and oils London, United Kingdom, 4-8 November 1996; Joint Fao/Who Food Standards Programme Codex Alimentarius Commission Twenty-second Session Geneva, 23-28 June 1997, CL 1997/2-FO January 1997. Available from: https://www.fao.org/input/download/report/313/al97_17e.pdf.
EFSA, 2015. Risk profile related to production and consumption of insects as food and feed. EFSA J 10:26-9.
Escamilla-Rosales MF, Ariza-Ortega JA, Ramos-Cassellis ME et al. 2019. Comparison of the proximal chemical and fatty acid composition of the fried grasshopper’s (Orthoptera) dish. Eur Food Res Technol 245:1629-40. DOI: https://doi.org/10.1007/s00217-019-03272-6
European Commission, 2011. Commission Regulation (EU) No 61/2011 of 24 January 2011 amending Regulation (EEC) No 2568/91 on the characteristics of olive oil and olive-residue oil and on the relevant methods of analysis. In: Official Journal, L 23, 27/01/2011.
European Commission, 2023. Commission Regulation (Eu) 2023/915 of 25 April 2023 on maximum levels for certain contaminants in food and repealing Regulation (EC) No 1881/2006. In: Official Journal, L 119/103, 05/05/2023.
Fasolato L, Cardazzo B, Carraro L, Fontana F, Novelli E, & Balzan S, 2018. Edible processed insects from e-commerce: food safety with a focus on the Bacillus cereus group. Food Microbiol 76:296-303. DOI: https://doi.org/10.1016/j.fm.2018.06.008
Fasolato L, Novelli E, Salmaso L, Corain L, Camin F, Perini M, Antonetti P, Balzan S, 2010. Application of nonparametric multivariate analyses to the authentication of wild and farmed European sea bass (Dicentrarchus labrax). Results of a survey on fish sampled in the retail trade. J Agric Food Chem 58:10979-88. DOI: https://doi.org/10.1021/jf1015126
Fombong FT, Van Der Borght M, Vanden Broeck J, 2017. Influence of freeze-drying and oven-drying post blanching on the nutrient composition of the edible insect Ruspolia differens. Insects 8:102. DOI: https://doi.org/10.3390/insects8030102
Huang X, Ahn DU, 2019. Lipid oxidation and its implications to meat quality and human health. Food Sci Biotechnol 28:1275-85. DOI: https://doi.org/10.1007/s10068-019-00631-7
Jeon YH, Son YJ, Kim SH, Yun EY, Kang HJ, Hwang IK, 2016. Physicochemical properties and oxidative stabilities of mealworm (Tenebrio molitor) oils under different roasting conditions, Food Sci Biotechnol 25:105-10. DOI: https://doi.org/10.1007/s10068-016-0015-9
Jia F, Hu F, Wang J, 2011. Effects of thermal stress on lipid peroxidation and antioxidant enzyme activities of oriental fruitfly, Bactrocera dorsalis (Diptera: Tephritidae). Fla Entomol 94:956-63. DOI: https://doi.org/10.1653/024.094.0432
Ju R, Wei H, Wang F, ZHOU XH, LI B, 2014. Anaerobic respiration and antioxidant responses of Corythucha ciliata (Say) adults to heat-induced oxidative stress under laboratory and field conditions. Cell Stress and Chaperones 19:255-62. DOI: https://doi.org/10.1007/s12192-013-0451-x
Kamau E, Mutungi C, Kinyuru J, Imathiu S, Tanga C, Affognon H, Ekesi S, Nakimbugwe D, Fiaboe, KKM, 2018. Effect of packaging material, storage temperature and duration on the quality of semi-processed adult house cricket meal. J Food Res 7:21-31. DOI: https://doi.org/10.5539/jfr.v7n1p21
Kim E, Lim J, Chang Y, An S, Ahn MY, 2015. Changes in the quality characteristics of cricket (Gryllus bimaculatus) under various processing conditions. Korean J Food Preserv 22:218-24. DOI: https://doi.org/10.11002/kjfp.2015.22.2.218
Kim TJ, Silva JL, Chamul RS, Chen TC, 2000. Influence of ozone, hydrogen peroxide, or salt on microbial profile, TBARS and color of channel catfish fillets. J Food Sci 65:1210-3. DOI: https://doi.org/10.1111/j.1365-2621.2000.tb10267.x
Koc K, Incekara U, Turkez H, 2014. Biomonitoring of the genotoxic effects and oxidative potentials of commercial edible dung beetles (Onitis sp.), grasshopper (Caelifera sp.) and mole crickets (Gryllotalpa sp.) in vitro. Toxicol Ind Health 30:683-9. DOI: https://doi.org/10.1177/0748233712457451
Kolakowski BM, Johaniuk K, Zhang H, Yamamoto E, 2021. Analysis of microbiological and chemical hazards in edible insects available to canadian consumers. J Food Prot 84:1575-81. DOI: https://doi.org/10.4315/JFP-21-099
Ma W, Zhao X, Yin C, Jiang F, Du X, Chen T, Zhang Q, Qiu L, Xu H, Hull JJ, Li G, Sung WK, Li F, Lin Y, 2020. A chromosome‐level genome assembly reveals the genetic basis of cold tolerance in a notorious rice insect pest, Chilo suppressalis. Mol Ecol Resour 20:268-82. DOI: https://doi.org/10.1111/1755-0998.13078
Mason RP, Laporte JM, Andres S, 2000. Factors controlling the bioaccumulation of mercury, methylmercury, arsenic, selenium, and cadmium by freshwater invertebrates and fish. Arch Environ Contam Toxicol 38:283-97. DOI: https://doi.org/10.1007/s002449910038
McLeod RJ, Prosser CG, Wakefield JW, 2016. Identification of goat milk powder by manufacturer using multiple chemical parameters. J Dairy Sci 99:982-93. DOI: https://doi.org/10.3168/jds.2015-9627
Paul A, Frederich M, Caparros Megido R, Alabi T, Malik P, Uyttenbroeck R, Francis F, Blecker C, Haubruge E, Lognay G, Danthine S, 2017. Insect fatty acids: a comparison of lipids from three Orthopterans and Tenebrio molitor L. larvae. J Asia-Pac Entomol 20:337-40. DOI: https://doi.org/10.1016/j.aspen.2017.02.001
Poma G, Cuykx M, Amato E, Calaprice C, Focant JF, Covaci A, 2017. Evaluation of hazardous chemicals in edible insects and insect-based food intended for human consumption. Food Chem Toxicol 100:70-9. DOI: https://doi.org/10.1016/j.fct.2016.12.006
Reitznerová A, Šuleková M, Nagy J, Marcinčák S, Semjon B, Čertík M, Klempová T, 2017. Lipid peroxidation process in meat and meat products: a comparison study of malondialdehyde determination between modified 2-thiobarbituric acid spectrophotometric method and reverse-phase high-performance liquid chromatography. Molecules 16:1988. DOI: https://doi.org/10.3390/molecules22111988
Singh Y, Cullere M, Kovitvadhi A, Chundang P, Dalle Zotte A, 2020. Effect of different killing methods on physicochemical traits, nutritional characteristics, in vitro human digestibility and oxidative stability during storage of the house cricket (Acheta domesticus L.). IFSET 65:102444. DOI: https://doi.org/10.1016/j.ifset.2020.102444
Tiencheu B, Macaire Womeni H, Linder M, Tchouanguep Mbiapo F, Villeneuve P, Fanni, J, Parmentier, M, 2013. Changes of lipids in insect (Rhynchophorus phoenicis) during cooking and storage. Eur J Lipid Sci Tech 115:186-95. DOI: https://doi.org/10.1002/ejlt.201200284
Tomotake H, Katagiri M, Yamato M, 2010. Silkworm pupae (Bombyx mori) are new sources of high quality protein and lipid. J Nutr Sci Vitaminol 56:446-8. DOI: https://doi.org/10.3177/jnsv.56.446
Van Huis A, Van Itterbeeck J, Klunder H, Mertens E, Halloran A, Muir G, Vantomme P, 2013. Edible insects: future prospects for food and feed security. Available from: https://www.fao.org/4/i3253e/i3253e.pdf.
Wang D, Xiao H, Lyu X, Chen H, Wei F, 2023. Lipid oxidation in food science and nutritional health: A comprehensive review. Oil Crop Sci 8:35-44. DOI: https://doi.org/10.1016/j.ocsci.2023.02.002
Yang LF, Siriamornpun S, Li D, 2006. Polyunsaturated fatty acid content of edible insects in Thailand. J Food Lipids 13:277-85. DOI: https://doi.org/10.1111/j.1745-4522.2006.00051.x
Zhuang P, Zou H, Shu W, 2009. Biotransfer of heavy metals along a soil-plant-insect-chicken food chain: field study. J Environ Sci 21:849-53. DOI: https://doi.org/10.1016/S1001-0742(08)62351-7
Zielińska E, Baraniak B, Karaś M, Rybczyńska K, Jakubczyk A, 2015. Selected species of edible insects as a source of nutrient composition. Food Res Int 77:460-6. DOI: https://doi.org/10.1016/j.foodres.2015.09.008

How to Cite

1.
Balzan S, Fasolato L, Fontana F, Currò S, Novelli E. Insect-based products commercialized online: a snapshot of lipid oxidation and mineral content. Ital J Food Safety [Internet]. 2024 Aug. 6 [cited 2024 Nov. 15];. Available from: https://www.pagepressjournals.org/ijfs/article/view/11600
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