https://doi.org/10.4081/ijfs.2023.11074
A case study on DNA barcoding for pet food mislabeling in South Korea
Submitted: 8 December 2022
Accepted: 12 January 2023
Published: 8 March 2023
Accepted: 12 January 2023
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
Due to the close relationship between pets and humans, pet owners are highly invested in proper diets for their pets. Even though pet food mislabeling is concerning, there are few studies on this topic. This study investigated pet food mislabeling in South Korea’s market based on DNA barcoding. In total, 10 pet food products were purchased, and 200 sequences of the partial Cytochrome c oxidase subunit 1 (COI) gene were generated from clones of the samples. The obtained sequences were compared to available public databases to identify species present in the ingredients. The data analyses showed that the labeled species were consistent with species detected by COI sequences in 6 of the products. However, the expected species were not detected in 4 products, revealing possible mislabeling in these samples. Our findings indicated that DNA barcoding might represent a promising tool to detect pet food mislabeling.
Alaska Seafood Marketing Institute, 2016. Whitefish Buyers Guide. Available from: https://www.alaskaseafood.org/wp-content/uploads/23-017-Whitefish-Guide_update-July-2020_final.pdf.
American pet products association (APPA), 2019. American pet products associations: pet industry market size and ownership.
Armani A, Tinacci L, Xiong X, Castigliego L, Gianfaldoni D, Guidi A, 2015. Fish species identification in canned pet food by BLAST and forensically informative nucleotide sequencing (FINS) analysis of short fragments of the mitochondrial 16s ribosomal RNA gene (16S rRNA). Food Control 50:821–30.
DOI: https://doi.org/10.1016/j.foodcont.2014.10.018
Carvalho DC, Palhares RM, Drummond MG.and Frigo TB, 2015. DNA barcoding DNA barcoding methodologies for the identification of fish species in cooked products. Food Control 84:297–304.
DOI: https://doi.org/10.1016/j.foodcont.2017.08.013
Do TD, Choi TJ, Kim JI, An HE, Park YJ, Karagozlu MZ, Kim CB, 2019. Assessment of marine fish mislabelling in South Korea’s markets by DNA barcoding. Food Control 100:53–7.
DOI: https://doi.org/10.1016/j.foodcont.2019.01.002
European Pet Food Industry Federation (FEDIAF), 2019. Facts & figures 2019. European Pet Food Industry Federation, Brussels, Belgium. Available from: https://www.fecava.org/news-and-events/news/fediaf-european-facts-figures-2019/
Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R, 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol 3:294–9.
Handy SM, Deeds JR, Ivanova NV, Hebert P DN, Hanner R, Ormos A, Weigt L, Moore MM, Yancy HF, 2011. A single-laboratory validated method for the generation of DNA barcodes for the identification of fish for regulatory compliance. J AOAC Int 94:201-10.
DOI: https://doi.org/10.1093/jaoac/94.1.201
Hebert PDN, Stoeckle MY, Zemlak TS, Francis CM, 2004. Identification of birds through DNA barcodes. PLoS Biol 2:e312.
DOI: https://doi.org/10.1371/journal.pbio.0020312
Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, Thierer T, Ashton B, Meintjes P, Drummond A, 2012. Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28:1647–9.
DOI: https://doi.org/10.1093/bioinformatics/bts199
National Chicken Council (NCC), 2022. Wholesale and retail prices for chicken, beef, and pork. The National Chicken Council. Available from: at https://www.nationalchickencouncil.org/about-the-industry/statistics/wholesale-and-retail-prices-for-chicken-beef-and-pork/. Accessed on 10 February 2022.
Okuma TA, Hellberg RS, 2015. Identification of meat species in pet foods using a real-time polymerase chain reaction (PCR) assay. Food Control 50:9-17.
DOI: https://doi.org/10.1016/j.foodcont.2014.08.017
Pardo MÁ, Jiménez E, Viðarsson JR, Ólafsson K, Ólafsdóttir G, Daníelsdóttir AK, Villareal BP, 2018. DNA barcoding revealing mislabeling of seafood in European mass caterings. Food Control 92:7–16.
DOI: https://doi.org/10.1016/j.foodcont.2018.04.044
Premanandh J, 2013. Horse meat scandal - a wake-up call for regulatory authorities. Food Control 34:568-9.
DOI: https://doi.org/10.1016/j.foodcont.2013.05.033
Shackell GH, 2008. Traceability in the meat industry - the farm to plate continuum. Int J Food Sci 43:2134-42.
DOI: https://doi.org/10.1111/j.1365-2621.2008.01812.x
Shehata HR, Naaum A, Garduno RA, Hanner R, 2018. DNA barcoding as a regulatory tool for seafood authentication in Canada. Food Control 92:147–53.
DOI: https://doi.org/10.1016/j.foodcont.2018.04.045
Silva AJ, Kawalek M, Williams-Hill DM, Hellberg RS, 2020. PCR Cloning Combined with DNA barcoding enables partial identification of fish species in a mixed-species product. Front Ecol Evol 8:28.
DOI: https://doi.org/10.3389/fevo.2020.00028
Son SJ, Bae JM, Park SJ, Lee HJ, Lee HS, 2017. A new area of food industry; companion animal market. Food Sci Indu 50:92-103.
Ward RD, Zemlak TS, Innes BH, Last PR, Hebert PDN, 2005. DNA barcoding Australia's fish species. Philos Trans R Soc Lond B Biol Sci 360:1847-57.
DOI: https://doi.org/10.1098/rstb.2005.1716
Supporting Agencies
This study was funded by the National Research Foundation of Korea (NRF) under grant No. NRF-2016M3A9E1915578.How to Cite
1.
Lee D, Do TD, Baek J-W, Mun M-H, An H-E, Kim C-B. A case study on DNA barcoding for pet food mislabeling in South Korea. Ital J Food Safety [Internet]. 2023 Mar. 8 [cited 2024 Nov. 23];12(1). Available from: https://www.pagepressjournals.org/ijfs/article/view/11074
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