https://doi.org/10.4081/ijfs.2023.11048

Effectiveness of essential oils against dual-species biofilm of Listeria monocytogenes and Pseudomonas fluorescens in a Ricotta-based model system

Vol. 12 No. 1 (2023)
Submitted: 1 December 2022
Accepted: 13 February 2023
Published: 8 March 2023
Thymbra capitata (L.) Cav., Cinnamomum zeylanicum, Listeria monocytogenes, Pseudomonas fluorescens, Dairy facilities
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Authors

Francesca Maggio
https://orcid.org/0000-0001-5866-043X
Department of Bioscience and Technology for Food, Agriculture and Environment, Teramo, Italy.
Annalisa Serio
aserio@unite.it
https://orcid.org/0000-0002-0473-9318
Department of Bioscience and Technology for Food, Agriculture and Environment, Teramo, Italy.
Chiara Rossi
https://orcid.org/0000-0001-7481-9332
Department of Bioscience and Technology for Food, Agriculture and Environment, Teramo, Italy.
Chiara Purgatorio
https://orcid.org/0000-0002-3210-8812
Department of Bioscience and Technology for Food, Agriculture and Environment, Teramo, Italy.
Francesco Buccioni
Department of Bioscience and Technology for Food, Agriculture and Environment, Teramo, Italy.
Clemencia Chaves- López
Department of Bioscience and Technology for Food, Agriculture and Environment, Teramo, Italy.
Antonello Paparella
https://orcid.org/0000-0001-8479-7253
Department of Bioscience and Technology for Food, Agriculture and Environment, Teramo, Italy.

Biofilms represent an evolutionary form of life, which translates from life in free-living cells to a community lifestyle. In natural habitats, biofilms are a multispecies complex, where synergies or antagonisms can be established. For example, Listeria monocytogenes and Pseudomonas fluorescens are associated with a dual-species biofilm that is widespread in dairy plants. In food plants, multiple strategies are devised to control biofilms, including natural compounds such as essential oils (EOs). In this respect, this study evaluated the effectiveness of Thymbra capitata (L.) Cav. EO and Cinnamomum zeylanicum (CEO) against a dual-species biofilm of L. monocytogenes and P. fluorescens, mimicking dairy process conditions. Based on Minimum Inhibitory Concentrations results, the EOs concentration (10 µL/mL) was chosen for the antibiofilm assay at 12°C on polystyrene (PS), and stainless steel surfaces for 168 h, using a Ricotta-based model system as culture medium. Biofilm biomass was assessed by crystal violet staining, and the planktonic and sessile cells were quantified in terms of Log CFU/cm2. Results showed that CEO displayed the greatest antibiofilm activity, reducing significantly (P<0.05) P. fluorescens and L. monocytogenes sessile cells of about 2.5 and 2.8 Log CFU/cm2 after 72 h, respectively. However, L. monocytogenes gained the protection of P. fluorescens, evading CEO treatment and showing a minimal sessile cell reduction of 0.7 Log CFU/cm2 after 72 h. Considering the outcome of this study, CEO might have promising perspectives for applications in dairy facilities.

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How to Cite

1.
Maggio F, Serio A, Rossi C, Purgatorio C, Buccioni F, Chaves- López C, Paparella A. Effectiveness of essential oils against dual-species biofilm of <em>Listeria monocytogenes</em> and <em>Pseudomonas fluorescens</em> in a Ricotta-based model system. Ital J Food Safety [Internet]. 2023 Mar. 8 [cited 2024 Dec. 26];12(1). Available from: https://www.pagepressjournals.org/ijfs/article/view/11048
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