Evaluation of vacuum packaging for extending the shelf life of Sardinian fermented sausage

Submitted: 29 August 2022
Accepted: 24 December 2022
Published: 8 June 2023
Abstract Views: 1322
PDF: 251
HTML: 6
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

Salsiccia sarda or Sardinian fermented sausage is a traditional dry-fermented sausage included in the list of traditional food products of Sardinia (Italy). At the request of some producing plants, the possibility of extending the shelf life of the vacuum-packed product up to 120 days was evaluated. Manufacturing of 90 samples, representing 3 different batches of Sardinian fermented sausage was carried out in two producing plants (A and B). In the packaged product and subsequently every 30 days for four months (T0, T30, T60, T120), the following analyses were conducted on all samples: physicochemical characteristics, total aerobic mesophilic count, Enterobacteriaceae count, detection of Listeria monocytogenes, Salmonella spp., mesophilic lactic acid bacteria, and coagulase-positive Staphylococci. Moreover, surfaces in contact and surfaces not in contact with food were sampled in both producing plants. Sensory profile analysis was also performed for every analysis time. At the end of the extended shelf life, pH values were equal to 5.90±0.11 (producing plant A) and 5.61±0.29 (producing plant B). Water activity mean values at T120 were 0.894±0.02 (producing plant A) and 0.875±0.01 (producing plant B). L. monocytogenes was detected in 73.3% (33/45) of the samples from producing plant A, with mean levels of 1.12±0.76 log10 CFU/g. In producing plant B, L. monocytogenes was never detected. Enterobacteriaceae were detected in 91.1% (41/45) of samples in producing plant A with mean values of 3.15±1.21 log10 CFU/g, and in 35.5% (16/45) samples in producing plant B samples with mean values of 0.72±0.86 log10 CFU/g. Salmonella and Staphylococcus aureus were never detected. Regarding environmental samples, the sites that were most contaminated by L. monocytogenes were the bagging table (contact surface) and processing room floor drains (non-contact surface) with a prevalence of 50% each (8/16 positive samples for both sampling sites). Sensory analysis results showed that at T30 the overall sensory quality was at its highest; moreover, the visual-tactile aspect, the olfactory characteristics, the gustatory aspects, and the texture showed significant differences in samples throughout the shelf life, with a decreased intensity at 120 days of storage. Overall, the quality and sensory acceptance of the vacuum-packed Sardinian fermented sausage were not affected until 120 days of shelf-life. However, the possible contamination by L. monocytogenes calls attention to the hygienic management of the entire technological process. The environmental sampling was confirmed as a useful verification tool during control.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

Citations

Barbuti S, Parolari G, 2002. Validation of manufacturing process to control pathogenic bacteria in typi-cal dry fermented products. Meat Sci 87:264-73.
Bourdichon F, Betts R, Dufour C, Fanning S, Farber J, McClure P, Stavropoulou DA, Wemmenhove E, Zwietering MH, Winklerk A, 2021. Processing environment monitoring in low moisture food pro-duction facilties: are we looking for the right microorganisms? Int J Food Microbiol 356:109351. DOI: https://doi.org/10.1016/j.ijfoodmicro.2021.109351
Casquete R, Benit MJ, Martin A, Ruiz-Moyano S, 2012. Comparison of the effects of a commercial and an autochthonous Pediococcus acidilactici and Staphylococcus vitulus starter culture on the sen-sory and safety properties of a traditional Iberian dry-fermented sausage salchichón. J Food Sci Technol 47:1011-9. DOI: https://doi.org/10.1111/j.1365-2621.2011.02935.x
Cenci-Goga BT, Rossitto PV, Sechi P, Parmegiani S, Cambiotti V, Cullor JS, 2012. Effect of selected dairy starter cultures on microbiological, chemical and sensory characteristics of swine and venison (Dama dama) nitrite-free dry-cured sausages. Meat Sci 90:599-606. DOI: https://doi.org/10.1016/j.meatsci.2011.09.022
Daga E, Mannu L, Porcu S, Comunian R, Paba A, Scintu MF, 2007. Home-made dry sausages produced in Sardinia: an investigation on the microflora. Ital J Food Sci 19:297:308.
Flores J, 1997. Mediterranean vs northern European meat products. Processing technologies and main differences. Food Chem 59:505-10. DOI: https://doi.org/10.1016/S0308-8146(97)00011-3
EFSA 2019, Hadjicharalambous C., Grispold, L, Cenci Goga, B, 2019. Quantitative risk assessment of Lis-teria monocytogenes in a traditional RTE product. EFSA J 17:e170906. DOI: https://doi.org/10.2903/j.efsa.2019.e170906
EFSA Panel on Biological Hazards, 2020. Guidance on date marking and related food information: part 1 (date marking). EFSA J 2020;18:e06306. DOI: https://doi.org/10.2903/j.efsa.2020.6306
European Commission, 2005. Regulation (EC) No 2073/2005 of the European Parliament and Council of 15 November 2005 on microbiological criteria for foodstuffs. In: Official Journal, L 338/1, 22/12/2005.
European Commission, 2008. Regulation (EC) No 1333/2008 of the European Parliament and Council of 16 December 2008 on food additives. In: Official Journal, L 354/16, 31/12/2008.
Gram L, Bagge-Ravn D, Yuwai N, Gymoese P, Fonnesbech VB, 2007. Influence of food soiling matrix on cleaning and disinfection efficiency on surface attached Listeria monocytogenes. Food Control 18:1165-71. DOI: https://doi.org/10.1016/j.foodcont.2006.06.014
Greco M, Mazzette R, De Santis EPL, Corona A, Cosseddu AM, 2005. Evolution and identification of lac-tic acid bacteria isolated during the ripening of Sardinian sausages. Meat Sci 69:733–9. DOI: https://doi.org/10.1016/j.meatsci.2004.11.004
Hawken P, Weese JS, Friendship R, Warriner K, 2013. Longitudinal study of Clostridium difficile and Methicillin-resistant Staphylococcus aureus associated with pigs from weaning through to the end of processing. J Food Prot 76:624-30. DOI: https://doi.org/10.4315/0362-028X.JFP-12-330
Hernández-Hernández E, Ponce-Alquicira E, Jaramillo-Flores ME, Guerrero Legarreta I, 2009. Antioxi-dant effect rosemary (Rosmarinus officinalis L.) and oregano (Origanum vulgare L.) extracts on TBARS and colour of model raw pork batters. Meat Sci 81:410-7. DOI: https://doi.org/10.1016/j.meatsci.2008.09.004
Hugas M, Garriga M, Aymerich MT, Monfort JM, 1995. Inibition of Listeria in dry fermented sausages by the bacteriocinogenic Lactobacillus sakei CTC494. J Appl Microbiol 75:322-30. DOI: https://doi.org/10.1111/j.1365-2672.1995.tb03144.x
ICMSF, 2002. Microorganisms in Foods 7. Microbiological testing in food safety management, New York, Kluwer Academic/Plenum Publishers.
ISO, 1989. Sensory analysis: general guidance for the design of test rooms. Norm ISO 8589. Geneva: In-ternational Organization for Standardization Publications.
ISO, 1998. Microbiology of food and animal feeding stuffs — horizontal method for the enumeration of mesophilic lactic acid bacteria — colony-count technique at 30 degrees C. Norm ISO 15214:1998. Geneva: International Organization for Standardization Publications.
ISO, 2005. Sensory analysis: general guidance for the selection, training and monitoring of assessors – part 2: experts. Norm ISO 8586. Geneva: International Organization for Standardization Publica-tions.
ISO, 2013. Microbiology of the food chain - horizontal method for the enumeration of microorganisms - part 1: colony count at 30 °c by the pour plate technique. Norm ISO 4833-1:2013/Amd 1:2022. Geneva: International Organization for Standardization Publications.
ISO, 2016. Sensory analysis: methodology. General guidance for establishing a sensory profile. Norm ISO 13299. Geneva: International Organization for Standardization Publications.
ISO, 2017a. Microbiology of the food chain - horizontal method for the detection and enumeration of listeria monocytogenes and of listeria spp. - part 1: detection method. Norm ISO 11290-1:2017. Geneva: International Organization for Standardization Publications.
ISO, 2017b. Microbiology of the food chain - horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. - enumeration method. Norm ISO 11290-2:2017. Ge-neva: International Organization for Standardization Publications.
ISO, 2017c. Microbiology of the food chain - preparation of test samples, initial suspension and deci-mal dilutions for microbiological examination - part 1: general rules for the preparation of the in-itial suspension and decimal dilutions. Norm ISO 6887:2017. Geneva: International Organization for Standardization Publications.
ISO, 2017d. Microbiology of the food chain - horizontal method for the detection and enumeration of Enterobacteriaceae - part 2: colony-count technique. Norm ISO 21528:2017. Geneva: Interna-tional Organization for Standardization Publications.
ISO, 2020. Microbiology of the food chain - horizontal method for the detection, enumeration and serotyping of Salmonella - part 1: detection of Salmonella spp. Norm ISO 6579-1:2020. Geneva: International Organization for Standardization Publications.
ISO, 2021. Microbiology of the food chain — horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) — part 2: method using rabbit plasma fibrinogen agar medium. Norm ISO 6888-2:2021. Geneva: International Organization for Standardization Publications.
Italian Republic, 2020. Revision of the national list of traditional agri-food products. Ministerial Decree No. 350 of September 8, 1999. Regulation containing rules for the identification of traditional products under article 8, paragraph 1 of Legislative Decree No. 173 of April 30, 1998. Prot. N.0001375 of 10/02/2020.
Khorsandi A, Hadi Eskandari M, Aminlari M, Shekarforoush SS, Taghi Golmakani M, 2019. Shelf-life ex-tension of vacuum packed emulsion-type sausage using combination of natural antimicrobials. Food Control 104:139-46. DOI: https://doi.org/10.1016/j.foodcont.2019.04.040
King W, Raposa SM, Warshaw JE, Johnson AR, Klinger JD, Halbert DN, 1989. A new colorimetric nucleic acid hybridization assay for Listeria in foods. Int J Food Microbiol 8:225–32. DOI: https://doi.org/10.1016/0168-1605(89)90017-2
Macfie HJH, Bratchel N, Greenhoff K, Vallis LV, 1989. Designs to balance the effect of order of presen-tation and first order carry–over effects in hall tests. J Sens Stud 4:129-48. DOI: https://doi.org/10.1111/j.1745-459X.1989.tb00463.x
Malakauskas M, Jorgensen K, Nielsen EM, Ojeniyi B, Olsen JE, 2006. Isolation of Campylobacter spp. from a pig slaughterhouse and analysis of cross-contamination. Int J Food Microbiol 108:295-300. DOI: https://doi.org/10.1016/j.ijfoodmicro.2005.09.012
Mangia NP, Murgia MA, Garau G, Deiana P, 2007. Microbial characterization and hygienic evaluation of the salsiccia sarda [Article in Italian]. Ind Aliment Italy 46:533-6.
Mangia NP, Trani A, Di Luccia A, Faccia M, Gambacorta G, Fancello F, Deiana P, 2013. Effect of the use of autochthonous Lactobacillus curvatus, Lactobacillus plantarum and Staphylococcus xylosus strains on microbiological and biochemical properties of the Sardinian fermented sausage. Eur Food Res Technol 236:557–66. DOI: https://doi.org/10.1007/s00217-013-1915-z
Malley TJV, Butts J, Wiedmann M, 2015. Seek and destroy process: Listeria monocytogenes process controls in the ready-to-eat meat and poultry industry. J Food Prot 78:436-45. DOI: https://doi.org/10.4315/0362-028X.JFP-13-507
Mataragas M, Bellio A, Rovetto F, Astegiano S, Decastelli L, Cocolin L, 2015. Risk-based control of food-borne pathogens Listeria monocytogenes and Salmonella enterica in the Italian fermented sau-sages Cacciatore and Felino. Meat Sci 103:39-45. DOI: https://doi.org/10.1016/j.meatsci.2015.01.002
Mazza R, Piras F, Ladu D, Putzolu M, Consolati SG, Mazzette R, 2015. Identification of Listeria spp. strains isolated from meat products and meat production plants by multiplex polymerase chain reaction. Ital J Food Saf 4:5498. DOI: https://doi.org/10.4081/ijfs.2015.5498
Meloni D, 2015. Presence of Listeria monocytogenes in mediterranean-style dry fermented sausages. Foods 4:34-50. DOI: https://doi.org/10.3390/foods4010034
Meloni D, Galluzzo P, Mureddu A, Piras F, Griffiths M, Mazzette R, 2009. Listeria monocytogenes in RTE foods marketed in Italy: prevalence and automated EcoRI ribotyping of the isolates. Int J Food Microbiol 129:166-73. DOI: https://doi.org/10.1016/j.ijfoodmicro.2008.11.014
Montouto-Graña M, Fernandez-Fernandez E, Vazquezoderiz ML, Romero-Rodriguez MA, 2002. Devel-opment of a sensory profile for the specific denomination “galician potato”. Food Qual Prefer 13:99–106. DOI: https://doi.org/10.1016/S0950-3293(01)00066-0
Mureddu A, Mazza R, Fois F, Meloni D, Bacciu R, Piras F, Mazzette R, 2014. Listeria monocytogenes persistence in ready-to-eat sausages and in processing plants. Ital J Food Saf 3:1697. DOI: https://doi.org/10.4081/ijfs.2014.1697
Namvar A, Warriner K, 2006. Application of enterobacterial repetitive intergenic consensus-polymerase chain reaction to trace the fate of the generic Escherichia coli within a high capacity pork slaughter line. Int J Food Microbiol 108:155-63. DOI: https://doi.org/10.1016/j.ijfoodmicro.2005.11.006
Nyhan M, Begley A, Mutel Y, Qu N, Johnson M, Callanan C, 2018. Predicting the combinatorial effects of water activity, pH and organic acids on Listeria growth in media and complex food matrices. Food Microb 74:75-85. DOI: https://doi.org/10.1016/j.fm.2018.03.002
O’Neill CM, Cruz-Romero MC, Duffy G, Kerry JP, 2018. Shelf life extension of vacuum-packed salt re-duced frankfurters and cooked ham through the combined application of high-pressure pro-cessing and organic acids. Food Packag Shelf Life 17:120-8. DOI: https://doi.org/10.1016/j.fpsl.2018.06.008
Pagliarini E, 2002. Valutazione Sensoriale. Aspetti teorici, pratici e metodologici. Milan; Italy: Hoepli. pp. 83–96.
Parra V, Viguer J, Sanchez J, Peinado J, Esparrago F, Gutierrez J, Andrés AI, 2010. Modified atmosphere packaging and vacuum packaging for long period chilled storage of dry-cured Iberian ham. Meat Sci 84:760-8. DOI: https://doi.org/10.1016/j.meatsci.2009.11.013
Pedonese F, Torracca B, Mancini S, Pisano S, Turchi B, Cerri D, Nuvoloni R, 2020. Effect of a Lactobacil-lus sakei and Staphylococcus xylosus protective culture on Listeria monocytogenes growth and quality traits of Italian fresh sausage (salsiccia) stored at abusive temperature. Ital J Anim Sci 19:1363-74. DOI: https://doi.org/10.1080/1828051X.2020.1844084
Petrón MJ, Broncano JM, Otte J, Martín L, Timón ML, 2013. Effect of commercial proteases on shelf-life extension of Iberian dry-cured sausage. Lwt-Food Sci Technol 53:191-7. DOI: https://doi.org/10.1016/j.lwt.2013.02.014
Piras F, Spanu C, Mocci AM, Demontis M, De Santis EPL, Scarano C, 2019. Occurrence and traceability of Salmonella spp. in five SFS facilities. Ital J Food Saf 8:8011. DOI: https://doi.org/10.4081/ijfs.2019.8011
Reddy PK, Rao JB, 2000. Effect of binders and pre-cooking meat on quality of chicken loaves. J Food Sci Technol 37:551–3.
Rubio B, Martínez B, García-Cachán MD, Rovira J, Jaime I. Effect of the packaging method and the stor-age time on lipid oxidation and colour stability on dry fermented sausage salchichón manufac-tured with raw material with a high level of mono and polyunsaturated fatty acids. Meat Sci. 2008;80:1182–1187. DOI: https://doi.org/10.1016/j.meatsci.2008.05.012
Ryu J, Park SH, Yeom YS, Shrivastav A, Lee SH, Kim YR, Kim HY, 2013. Simultaneous detection of Lis-teria species isolates from meat processed foods using multiplex PCR. Food Control 32:659-64. DOI: https://doi.org/10.1016/j.foodcont.2013.01.048
Saraiva C, Garcia-Diez J, da Conceição-Fontes M, Esteves A, 2018. Modeling the behavior of Listeria monocytogenes in meat. In: Nyila MA (ed). Listeria monocytogenes. Available from: http://dx.doi.org/10.5772/intechopen.71807. DOI: https://doi.org/10.5772/intechopen.79967
Sčetar M, Kovačić E, Kurek M, Galić K, 2013. Shelf life of packaged sliced dry fermented sausage under different temperature. Meat Sci 93:802-9. DOI: https://doi.org/10.1016/j.meatsci.2012.11.051
Siddi G, Piras F, Spanu V, Meloni MP, Sanna R, Carta N, Errico M, Cuccu M, De Santis EPL, Scarano C, 2022. Selection of commercial protective cultures to be added in Sardinian fermented sausage to control Listeria monocytogenes. Ital J Food Saf 11:10368. DOI: https://doi.org/10.4081/ijfs.2022.10368
Simmons CK, Wiedmann M, 2018. Identification and classification of sampling sites for pathogen envi-ronmental monitoring programs for Listeria monocytogenes: results from an expert elicitation. Food Microbiol 75:2-17. DOI: https://doi.org/10.1016/j.fm.2017.07.005
Spanu C, Jordan K, 2020. Listeria monocytogenes environmental sampling program in ready‐to‐eat processing facilities: a practical approach. Compr Rev Food Sci F 19:2843-61. DOI: https://doi.org/10.1111/1541-4337.12619
Thévenot D, Delignette-Muller ML, Christieans S, Vernozy-Rozand C, 2005. Fate of Listeria monocyto-genes in experimentally contaminated French sausages. Int J Food Microbiol 101:189–200. DOI: https://doi.org/10.1016/j.ijfoodmicro.2004.11.006
Tompkin RB, Scott VN, Bernard DT, Sveum WH, Gombas KS, 1999. Guidelines to prevent post-processing contamination from Listeria monocytogenes. Dairy Food Environ Sanitation 19:551-62.
Vercammen A, Vanoirbeek KGA, Lurquin I, Steen L, Goemaere O, Szczepaniak S, Paelinck H, Hendrickx MEG, Michiels CW, 2011. Shelf-life extension of cooked ham model product by high hydrostatic pressure and natural preservatives. Innov Food Sci Emerg Technol 12:407-15. DOI: https://doi.org/10.1016/j.ifset.2011.07.009
Zagorec M, Champomier-Vergès MC, 2017. Lactobacillus sakei: a starter for sausage fermentation, a protective culture for meat products. Microorganisms 5:56. DOI: https://doi.org/10.3390/microorganisms5030056

How to Cite

1.
Siddi G, Piras F, Meloni MP, Casti D, Spanu C, Pala C, Mocci AM, Piga C, Di Salvo R, De Santis E, Scarano C. Evaluation of vacuum packaging for extending the shelf life of Sardinian fermented sausage. Ital J Food Safety [Internet]. 2023 Jun. 8 [cited 2024 Nov. 21];12(2). Available from: https://www.pagepressjournals.org/ijfs/article/view/10819