https://doi.org/10.4081/ijfs.2022.10510

Antimicrobial of tropical fruit and vegetable waste extract for food-borne pathogenic bacteria

Vol. 11 No. 3 (2022)
Submitted: 12 April 2022
Accepted: 1 September 2022
Published: 8 September 2022
Antimicrobial, Fruit, Tropical, Vegetable, Waste
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Authors

Titik Budiati
titik_budiati@polije.ac.id
Food Engineering Department, Politeknik Negeri Jember, Jember, East Java, Indonesia.
Wahyu Suryaningsih
Food Engineering Department, Politeknik Negeri Jember, Jember, East Java, Indonesia.
Titania Nur Bethiana
Chemical and Biomolecular Engineering Department, Johns Hopkins University, Baltimore, MD, United States.

Tropical fruit and vegetable wastes become great potential natural resources of bioactive compounds for antimicrobial. The aim of the study was to determine the effect of antimicrobial extracted from tropical fruit and vegetable waste to inhibit foodborne pathogenic bacteria (Aeromonas hydrophilla, Bacillus cereus, Escherichia coli, Listeria monocytogenes, Salmonella Typhimurium, Staphylococcus aureus, Vibrio parahaemolyticus). A total of six tropical fruit waste (peel of pineapple, jackfruit, durian, coffee, mangosteen, and cacao pods) and five tropical vegetable waste (stem of sembukan, lamtoro pods, jengkol shell, bitter bean pods, Indian marsh fleabane leave) was extracted by using maceration method. The antimicrobial activity of extracts was carried out by using disc diffusion assay and Minimum Inhibitory Concentration. The flavonoids in extract were identified and quantified by using Liquid Chromatography-Mass Spectrometry. The highest antimicrobial activity against Gram-positive bacteria (B. cereus, L. monocytogenes and S. aureus) was shown by jengkol, bitter bean and mangosteen waste extract in the range of 0,00038 to 4,2% for MIC. The highest antimicrobial activity inhibits Gram-negative bacteria (A. hydrophilla, E. coli, S. Typhimurium and V. parahaemolyticus) was shown by jengkol, bitter bean, mangosteen, sembukan and lamtoro waste extract in the range of 0,00038 to 3,1% for MIC which have apigenin, catechin, coumaric acid, gallic acid, genistein, hydroxybenzoic acids, luteolin, myricetin, naringenin dan quercetin as bioactive compounds. Total phenol of those waste extracts was in the range of 0.663 to 4,441 mg GAE/g. Jengkol, bitter bean, mangosteen, sembukan and lamtoro waste extract shown to be a potential natural antimicrobial to inhibit food-borne pathogenic bacteria.

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How to Cite

1.
Budiati T, Suryaningsih W, Nur Bethiana T. Antimicrobial of tropical fruit and vegetable waste extract for food-borne pathogenic bacteria. Ital J Food Safety [Internet]. 2022 Sep. 8 [cited 2024 Nov. 23];11(3). Available from: https://www.pagepressjournals.org/ijfs/article/view/10510

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