https://doi.org/10.4081/ijfs.2022.10412
DNA metabarcoding for identification of species used in fish burgers
Submitted: 15 February 2022
Accepted: 23 May 2022
Published: 11 August 2022
Accepted: 23 May 2022
Abstract Views: 1072
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Supplementary Tables: 59
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Supplementary Tables: 59
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
The absence of morphological identification characters, together with the complexity of the fish supply chain make processed seafood vulnerable to cases of species substitution. Therefore, the authentication and the traceability of such products play a strategic role in ensuring quality and safety. The aim of the present study was to detect species used in the production of multi-species fish burgers and to evaluate mislabelling rates, using a DNA metabarcoding approach by sequencing a fragment of the 16S rRNA mitochondrial gene. The study highlighted the presence of 16 marine and 2 mammalian taxa with an overall mislabelling rate of 80%, including cases of species substitution, the undeclared presence of molluscs and of taxa whose use is not permitted by current Italian legislation. The presence of swine DNA as well as the inclusion of undeclared taxa potentially causing allergies raise concerns regarding consumer safety and protection regarding ethical or religious issues. Overall, the study shows that the application of DNA metabarcoding is a promising approach for successfully enforcing traceability systems targeting multi-species processed food and for supporting control activities, as a guarantee of an innovative food safety management system.
Barbuto M, Galimberti A, Ferri E, Labra M, Malandra R, Galli P, Casiraghi M, 2010. DNA barcoding reveals fraudulent substitutions in shark seafood products: The Italian case of “palombo” (Mustelus spp.). Food Res Int 43:376–81.
DOI: https://doi.org/10.1016/j.foodres.2009.10.009
Bruno A, Sandionigi A, Agostinetto G, Bernabovi L, Frigerio J, Casiraghi M, Labra M, 2019. Food Tracking Perspective: DNA Metabarcoding to Identify Plant Composition in Complex and Processed Food Products. Genes 10:248
DOI: https://doi.org/10.3390/genes10030248
Callahan BJ, McMurdie PJ, Rosen MJ, Han AW, Johnson AJA, Holmes SP, 2016. DADA2: high-resolution sample inference from Illumina amplicon data. Nature Methods 13:581-3.
DOI: https://doi.org/10.1038/nmeth.3869
Camacho C, Coulouris G, Avagyan V, Ma N, Papadopoulos J, Bealer K, Madden TL, 2009. BLAST+: Architecture and applications. BioMed Central Bioinformatics 10:421.
DOI: https://doi.org/10.1186/1471-2105-10-421
Caponetto P, Fischer J, Biedermann T, 2013. Gelatin-containing sweets can elicit anaphylaxis in a patient with sensitization to galactose-α-1,3-galactose. J Allergy Clin Immunol Pract 1:302-3.
DOI: https://doi.org/10.1016/j.jaip.2013.01.007
Chapela MJ, Sotelo CG, Calo-Mata P, Pérez-Martín RI, Rehbein H, Hold GL, Quinteiro J, Rey-Méndez M, Rosa C, Santos AT, 2002. Identification of cephalopod species (Ommastrephidae and Loliginidae) in seafood products by Forensically informative Nucleotide sequencing (FINS). J Food Sci 67:1672-6.
DOI: https://doi.org/10.1111/j.1365-2621.2002.tb08703.x
D’Amico P, Armani A, Gianfaldoni A, Guidi A, 2016. New provisions for the labelling of fishery and aquaculture products: Difficulties in the implementation of Regulation (EU) n. 1379/2013. Mar Policy 71:147-56.
DOI: https://doi.org/10.1016/j.marpol.2016.05.026
Di Pinto A, Mottola A, Marchetti P, Savarino A, Tantillo G, 2019. Fraudulent species substitution in e-commerce of protected denomination origin (PDO) products. J Food Compos Anal 79:143-7.
DOI: https://doi.org/10.1016/j.jfca.2019.03.018
FAO, 2018. Overview of food fraud in the fisheries sector. Available at: https://www.fao.org/3/I8791EN/i8791en.pdf. Accessed on 03 December 2021.
FAO,2020a. Summary of the impacts of the covid-19 pandemic on the fisheries and aquaculture sector. Available at: https://www.fao.org/3/ca9349en/CA9349EN.pdf. Accessed on 03 December 2021.
FAO. 2020b. The State of World Fisheries and Aquaculture 2020. Sustainability in action. Rome. Available at: https://doi.org/10.4060/ca9229en. Accessed on 03 December 2021.
DOI: https://doi.org/10.4060/ca9229en
Giusti A, Armani A, Sotelo CG, 2017. Advances in the analysis of complex food matrices: Species identification in surimi-based products using Next Generation Sequencing technologies. PLoS One 12:1-18.
DOI: https://doi.org/10.1371/journal.pone.0185586
Husein Y, 2019. Application of the mechanical separation process in different fish species for the development of a new product based on fish. Italy: PhD Thesis, University of Firenze.
Huson DH, Auch AF, Qi J, Schuster SC, 2007. MEGAN analysis of metagenomic data. Genome Res 17:377-86.
DOI: https://doi.org/10.1101/gr.5969107
Marchetti P, Mottola A, Piredda R, Ciccarese G, Di Pinto A., 2020. Determining the authenticity of shark meat products by DNA sequencing. Foods 9:1194.
DOI: https://doi.org/10.3390/foods9091194
Milla L, Sniderman K, Lines R, Mousavi‐Derazmahalleh M, Encinas‐Viso F. Pollen DNA metabarcoding identifies regional provenance and high plant diversity in Australian honey. Ecol Evol 11:8683-98.
DOI: https://doi.org/10.1002/ece3.7679
Mottola A, Piredda R, Catanese G, Lorusso L, Ciccarese G, Di Pinto A, 2022. Species authentication of canned mackerel: Challenges in molecular identification and potential drivers of mislabelling. Food Control, 208880.
DOI: https://doi.org/10.1016/j.foodcont.2022.108880
Nitsuwat S, Zhang P, Ng K, Fang Z, 2021. Fish gelatin as an alternative to mammalian gelatin for food industry: A meta-analysis. LWT-Food Sci Technol 141:110899.
DOI: https://doi.org/10.1016/j.lwt.2021.110899
Noh ES, Lee M-N, Kim EM, Nam B-H, Noh JK, Park JY, Kim KH, Kang JH, 2021. Discrimination of raw material species in mixed seafood products (surimi) using the next generation sequencing method. Food Biosci 41: 100786.
DOI: https://doi.org/10.1016/j.fbio.2020.100786
Paracchini V, Petrillo M, Lievens A, Kagkli DM, Angers-Loustau A., 2019. Nuclear DNA barcodes for cod identification in mildly-treated and processed food products. Food Addit Contam A 36:1-14.
DOI: https://doi.org/10.1080/19440049.2018.1556402
Pardo MA, Jiménez E, Pérez-Villarreal B, 2016. Misdescription incidents in seafood sector. Food Control 62:277-83.
DOI: https://doi.org/10.1016/j.foodcont.2015.10.048
Piredda R, Mottola A, Cipriano G, Carlucci R, Ciccarese G, Di Pinto A, 2022. Next Generation Sequencing (NGS) approach applied to species identification in mixed processed seafood products. Food Control 133:108590.
DOI: https://doi.org/10.1016/j.foodcont.2021.108590
Preckel L, Brünen-Nieweler C, Denay G, Petersen H, Cichna-Markl M, Dobrovolny S, Hochegger R, 2021. Identification of Mammalian and Poultry Species in Food and Pet Food Samples Using 16S rDNA Metabarcoding. Foods 10:2875.
DOI: https://doi.org/10.3390/foods10112875
Raclariu AC, Heinrich M, Ichim MC, de Boer H, 2018. Benefits and limitations of DNA barcoding and metabarcoding in herbal product authentication. Phytochem Analysis. 29:123-8.
DOI: https://doi.org/10.1002/pca.2732
Seethapathy GS, Raclariu-Manolica AC, Anmarkrud JA, Wangensteen H, de Boer HJ, 2019. DNA Metabarcoding Authentication of Ayurvedic Herbal Products on the European Market Raises Concerns of Quality and Fidelity. Front Plant Sci 10:68.
DOI: https://doi.org/10.3389/fpls.2019.00068
Varunjikar MS, Moreno-Ibarguen C, Andrade-Martinez JS, Tung H-S Palmblad IBM, Olsvik PA, Reyes A, Rasinger JD, Lie KK, 2022. Comparing novel shotgun DNA sequencing and state-of-the-art proteomics approaches for authentication of fish species in mixed samples. Food Control 131:108417.
DOI: https://doi.org/10.1016/j.foodcont.2021.108417
Walker CC, Lassitter CL, Lynn SN, Ford CB, Rademacher KR, Ruple AD, Bell JW, 2017. Rapid Seafood Species Identification Using Chip-Based Capillary Electrophoresis and Protein Pattern Matching. J Aoac Int 100:1500-10.
DOI: https://doi.org/10.5740/jaoacint.17-0178
Xing RR, Wang N, Hu RR, Zhang JK, Han JX, Chen Y., 2019. Application of next generation sequencing for species identification in meat and poultry products: A DNA metabarcoding approach. Food Control,101:173-9.
DOI: https://doi.org/10.1016/j.foodcont.2019.02.034
Zhou P, Feng Q, Yang X, Gao R, Li Y, Bai F, Wang J, Zhou X, Wang H, Xiao F, Yang X, Gao X, Zhao Y, 2021. Sous vide pretreatment in cooking sturgeon fish burger: Effects on physicochemical properties and sensory characteristics. Int J Food Sci Tech 56:2973-82.
DOI: https://doi.org/10.1111/ijfs.14938
Zin ZM, Sarbon NM, Zaino MK, Jaafar SN, Shukri MM, Rahman AHA, 2021. Halal and Non-Halal Gelatine as a Potential Animal By-Products in Food Systems: Prospects and Challenges for Muslim Community. Adv Social Sci Ed Hum Res 536:530-32.
DOI: https://doi.org/10.2991/assehr.k.210312.086
How to Cite
1.
Mottola A, Piredda R, Catanese G, Giorelli F, Cagnazzo G, Ciccarese G, Dambrosio A, Quaglia NC, Di Pinto A. DNA metabarcoding for identification of species used in fish burgers. Ital J Food Safety [Internet]. 2022 Aug. 11 [cited 2024 Dec. 3];11(3). Available from: https://www.pagepressjournals.org/ijfs/article/view/10412
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