https://doi.org/10.4081/ijfs.2022.10221

Occurrence of drug-resistant enteric bacteria and associated factors among food handlers of the Mass Catering Center: A laboratory-based cross-sectional study

Vol. 11 No. 3 (2022)
Submitted: 16 October 2021
Accepted: 2 March 2022
Published: 31 August 2022
Antimicrobial-resistance, Mass catering center, Foodborne outbreak
Abstract Views: 597
PDF: 226
HTML: 17
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

Chalachew Yenew
chalachewyenew50@gmail.com
https://orcid.org/0000-0003-1000-9220
Social and Population Health Unit, College of Medicine and Health Sciences, Debre Tabor University, Ethiopia.
Minwuyelet Andualem
Department of English Language and Literature, College of Social Sciences and Humanities, Debre Tabor University, Ethiopia.
Fitalew Tadele
Biochemistry, Biomedical Sciences, College of Medicine and Health Sciences, Debre Tabor University, Ethiopia.
Sileshi Mulatu
Pediatrics and Child Health Nursing, College of Medicine and Health Sciences, Bahir Dar University, Ethiopia.
Getaneh Atikilt
Social and Population Health Unit, College of Medicine and Health Sciences, Debre Tabor University, Ethiopia.
Asaye Alamneh Gebeyehu
Social and Population Health Unit, College of Medicine and Health Sciences, Debre Tabor University, Ethiopia.

This study aimed to determine antimicrobial- resistance (AMR) enteric bacterial load and associated factors among Food Handlers (FHs) ofMass Catering Center (MCC), Ethiopia. From January to June 2020, a laboratory-based cross-sectional study was conducted using the standard Swab microbiological analysis method and the Kirby-Bauer disc diffusion method on 160 randomly selected FHs and food serving areas and analyzed with RStudio- 1.2.5033 for contamination, resistance level, and source determination. The contamination of AMR Escherichia coli was 33.3% (95% CI, 31.5%, 36.4%), Salmonella 30% (95% CI, 29.8%, 36.0%) and Shigella 20% (95% CI, 19.2%, 26.9%) among the hand of FHs serving in MCC. The contamination was more likely associated with low work experience [AOR, 1.42 (95% CI: 1.22-1.87)], a low educational level [AOR, 1.62 (95% CI: 1.52-189)], irrational drug use characteristics [AOR, 1.75(95% CI: 1.64-2.00)], lack of sufficient food safety knowledge [AOR, 1.52 (95% CI: 1.32-1, 67)] of the FHs and the sanitary condition of the food serving area [AOR:1.98 (95% CI, 1.45, 2.53)]. The contamination of the hand of FHs serving in the MCC with AMR enteric FBB at the University cafeteria could indicate the likelihood of the occurrence of foodborne outbreaks.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

Citations

Crossref
Scopus
Google Scholar
Europe PMC
Clinical and Laboratory Standards Institute, 2016. Performance Standards for Antimicrobial Susceptibility Testing Supplement M100S. 26th ed.
Diriba K, Awulachew E, Ashuro Z, 2020. Prevalence and Antimicrobial Resistance Pattern of Salmonella, Shigella, and Intestinal Parasites and Associated Factor among Food Handlers in Dilla University Student Cafeteria, Dilla, Ethiopia. Int J Microbiol 2020: 3150539. DOI: https://doi.org/10.1155/2020/3150539
Gebreegziabher G, Asrat D, W/Amanuel Y, Hagos T, 2018. Isolation and Antimicrobial Susceptibility Profile of Shigella and Salmonella Species from Children with Acute Diarrhoea in Mekelle Hospital and Semen Health Center, Ethiopia. Ethiop J Health Sci 28:197-206 DOI: https://doi.org/10.4314/ejhs.v28i2.11
Hussen S, Mulatu G, Yohannes Kassa Z, 2019. Prevalence of Shigella species and its drug resistance pattern in Ethiopia : a systematic review and meta-analysis. Ann Clin Microbiol Antimicrob 18:1–11. DOI: https://doi.org/10.1186/s12941-019-0321-1
ISO, 2001. ISO 16654 :2001. 1st ed. Microbiology – Horizontal method for the detection of Escherichia coli O157, International Organization for Standardization, Geneve, Switzerland. doi: 10.1136/bmjopen-2018-026517. DOI: https://doi.org/10.1136/bmjopen-2018-026517
Mahmud T, Hassan MM, Alam M, Khan MM, Bari MS, Islam A, 2016. Prevalence and multidrug-resistant pattern of Salmonella from the eggs and egg-storing trays of retail markets of Bangladesh. Int J One Health 2:7–11. DOI: https://doi.org/10.14202/IJOH.2016.7-11
Mama M, Alemu G, 2016. Prevalence , antimicrobial susceptibility patterns and associated risk factors of Shigella and Salmonella among food handlers in Arba Minch University, South. BMC Infect Dis 16:686. DOI: https://doi.org/10.1186/s12879-016-2035-8
Mamza S, et al., 2010. S. aureus isolated from chickens in Nigeria. 46:155–66.
Marshall BM, Levy SB, 2011. Food animals and antimicrobials: Impacts on human health. Clin Microbiol Rev 24:718–33. DOI: https://doi.org/10.1128/CMR.00002-11
Meleko A, Henok A, Tefera W, Lamaro T, 2015. Assessment of the Sanitary Conditions of Catering Establishments and Food Safety Knowledge and Practices of Food Handlers in Addis Ababa University Students Cafeteria. Sci J Public Health 5:733–43. DOI: https://doi.org/10.11648/j.sjph.20150305.30
Mengist A, Mengistu G, Reta A, 2018. Prevalence and antimicrobial susceptibility pattern of Salmonella and Shigella among food handlers in catering establishments at Debre Markos University, Northwest Ethiopia. Int J Infect Dis 75:74–9. DOI: https://doi.org/10.1016/j.ijid.2018.08.008
Moges F, Endris M, Mulu A, Tessema B, Belyhun Y, Shiferaw Y, Huruy K, Unakal C, Kassu A, 2014. The growing challenges of antibacterial drug resistance in Ethiopia. J Glob Antimicrob Resist 2:148-154 DOI: https://doi.org/10.1016/j.jgar.2014.02.004
NSW Food Authority, 2020. Environmental swabbing. Available from: https://www.foodauthority.nsw.gov.au/about-us/science/food-risk-studies/environmental-swabbing
Saima, Samad A, Roomeela, Rizwan M, Abbas F, Yousaf M, Hassan Y, Naeem M, Zahid M, Pokryshko O, Diaconescu S, Saifullah S, 2018. Isolation and Identification of Shigella species from food and water samples of Quetta, Pakistan. Pure Appl Biol 7:227-35. DOI: https://doi.org/10.19045/bspab.2018.70027
Speck NL, 2015. Compendium of Methods for The Examination of Foods. p. 701. Available at: https://docplayer.es/39537427-Rev-costarr-salud-publica-2013-22-n-1-vol-22-enero-junio-2013-original-breve.html.
Tadesse HA, Gidey NB, Workelule K, Hailu H, Gidey S, Bsrat A, Taddele H, 2018. Antimicrobial Resistance Profile of E. Coli Isolate from Raw Cow Milk and Fresh Fruit Juice in Mekelle, Tigray, Ethiopia. Vet Med Int 2018:8903142. DOI: https://doi.org/10.1155/2018/8903142
WHO, 2012. The evolving threat of antimicrobial resistance options for action. WHO Library Cataloguing-in-Publication Data.
WHO, UNICEF, 2012. Drinking Water. Available from: https://apps.who.int/iris/handle/10665/44842
Wolde T, Abate M, Sileshi H, Mekonnen Y, 2016. Prevalence and antimicrobial susceptibility profile of Salmonella species from ready-to-eat foods from catering establishments in Jigjiga City, Ethiopia. Afr J Microbiol 10:1555–60. DOI: https://doi.org/10.5897/AJMR2016.7944
Younis RI, Nasef SA, Salem WM, 2019. Detection of Multi-Drug Resistant Food-borne Bacteria in Ready-to-Eat Meat Products in Luxor City, Egypt. SVU Int J Vet Sci 2:20–35. DOI: https://doi.org/10.21608/svu.2019.23168

How to Cite

1.
Yenew C, Andualem M, Tadele F, Mulatu S, Atikilt G, Gebeyehu AA. Occurrence of drug-resistant enteric bacteria and associated factors among food handlers of the Mass Catering Center: A laboratory-based cross-sectional study. Ital J Food Safety [Internet]. 2022 Aug. 31 [cited 2024 Jul. 23];11(3). Available from: https://www.pagepressjournals.org/ijfs/article/view/10221

PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.