https://doi.org/10.4081/aiua.2023.11610

Are elevated mitochondrial DNA fragments in prostatic inflammation a potential biomarker for prostate cancer?

Vol. 95 No. 3 (2023)
Submitted: July 26, 2023
Accepted: September 2, 2023
Published: September 27, 2023
Prostate, inflammation, cancer, mitochondrial DNA, post-prostatic massage
Abstract Views: 617
PDF: 382
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Authors

Ugur Aferin
Department of Urology, Medical Faculty, Demiroglu Bilim University, Istanbul, Turkey.
Nurten Bahtiyar
nurten.bahtiyar@iuc.edu.tr
https://orcid.org/0000-0003-2420-8415
Department of Biophysics, Cerrahpasa Medical Faculty, Istanbul University-Cerrahpasa, Istanbul, Turkey.
Ilhan Onaran
https://orcid.org/0000-0003-4630-9516
Department of Medical Biology, Cerrahpasa Medical Faculty, Istanbul University-Cerrahpasa, Istanbul, Turkey.
Hamdi Ozkara
Department of Urology, Cerrahpasa Medical Faculty, Istanbul University-Cerrahpasa, Istanbul, Turkey.

Background: We sought to determine whether two soluble forms with different size of mtDNA are linked to prostatic inflammation, and whether they discriminate prostate cancer (PCa) from inflammatory prostatic conditions. Methods: Histopathologically diagnosed prostatitis, PCa and benign prostatic hyperplasia patients (n = 93) were enrolled in this study and they were categorized as with and without prostate inflammation. Quantitative RT-PCR was used to analyze the levels of 79-bp and 230-bp fragments in urine and blood samples collected following prostate massage. Results: The urine mtDNA-79 and mtDNA-230 were significantly increased in patients with prostate inflammation compared with those in without inflammation. Here, 79-bp fragment of apoptotic origin was significantly higher level than 230-bp fragment of necrotic origin. Although mtDNA-79 copy number in serum samples was also increased in patients with prostate inflammation, mtDNA-230 was similar in the two groups. Furthermore, mtDNA-79 and mtDNA-230 copy numbers in postprostate massage urine were higher (about 16-fold and 22-fold, respectively) than those from serum samples. ROC analysis showed that, although post-prostate massage urine have relatively higher performance than blood, ability to discriminate cases of both fragments was not better than that of serum total PSA. Conclusions: Our results demonstrate that shorter cf-mtDNA fragment size in particular, increase in the presence of prostate inflammation in post-prostatic massage urine but both fragments could never improve serum total PSA performance.

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Supporting Agencies

Istanbul University-Cerrahpasa

How to Cite

Aferin, U., Bahtiyar, N., Onaran, I., & Ozkara, H. (2023). Are elevated mitochondrial DNA fragments in prostatic inflammation a potential biomarker for prostate cancer?. Archivio Italiano Di Urologia E Andrologia, 95(3). https://doi.org/10.4081/aiua.2023.11610
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