https://doi.org/10.4081/mm.2006.2954

Valutazione di un test per la determinazione simultanea degli anticorpi e antigene p24 dell’HIV

Vol. 21 No. 1 (2006)
Submitted: 24 February 2014
Accepted: 24 February 2014
Published: 31 March 2006
HIV, 4th generation assay, p24, anti-HIV, screening
Abstract Views: 872
PDF: 1272
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

Nadia Zanchetta
viro.microbiologia@hsacco.it
Carla Bossi
Claudia Tonielli
Claudio Galli
A fourth generation immunoassay for the detection of antibodies to HIV-1 and HIV-2 and of HIV antigen p24 (Abbott Architect HIV Ag/Ab Combo) has been evaluated in comparison with two Ab-only third generation assays on 894 routine specimens and on preselected repository specimens.The Combo assay showed a better specificity (99.88% vs. 99.43-99.83%) and an analytical sensitivity for p24 of 22 pg/mL on a BBI commercial panel. The Architect assays gave a negative result on 22/24 repository false positive specimens from 18 subjects and, conversely, was positive on all the 39 repository specimens from 24 HIV-positive patients. On six patients with acute HIV infection the Architect assay gave an earlier positivity than the antibody-only assays (EIA and WB) on three cases, all viremic and positive for HIV p24.The performance characteristics of the new HIV Combo assay guarantee an advanced clinical sensitivity and a high specificity.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

Citations

Crossref
Scopus
Google Scholar
Europe PMC

How to Cite

Zanchetta, N., Bossi, C., Tonielli, C., & Galli, C. (2006). Valutazione di un test per la determinazione simultanea degli anticorpi e antigene p24 dell’HIV. Microbiologia Medica, 21(1). https://doi.org/10.4081/mm.2006.2954

PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.