An easy and inexpensive method to expose adhering cultured cells to ozonization


https://doi.org/10.4081/microscopie.2015.5164
Vol. 23 No. 1 (2015)
Submitted: 19 March 2015
Accepted: 19 March 2015
Published: 19 March 2015
Ozone, in vitro culture, epithelial cells
Abstract Views: 854
PDF: 1063
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

  • M. Costanzo
    manuela.costanzo@univr.it
    Department of Neurological and Movement Sciences, Anatomy and Histology Section, University of Verona, Italy.
  • B. Cisterna Department of Neurological and Movement Sciences, Anatomy and Histology Section, University of Verona, Italy.
  • V. Covi San Rocco Clinic, Montichiari, Brescia, Italy.
  • G. Tabaracci San Rocco Clinic, Montichiari, Brescia, Italy.
  • M. Malatesta Department of Neurological and Movement Sciences, Anatomy and Histology Section, University of Verona, Italy.
The regeneration capabilities of low ozone (O3) concentrations are usually applied for therapeutic purposes; however, the biological mechanisms accounting for the positive effects of mild ozonization are still largely unknown. Due to the high reactivity of O3, the study of the cellular effects of ozonization requires controlled and reproducible experimental conditions. An experimental procedure for treating cell suspensions with O3 has been already set up; however, it is hardly suitable for adhering cells such as epithelial, connective, muscular and neuronal cells. In the present work, we describe a new, easy and cheap method that, without requiring special equipment, proved to be suitable for treating cell monolayers with low O3 concentrations.

Costanzo, M., Cisterna, B., Covi, V., Tabaracci, G., & Malatesta, M. (2015). An easy and inexpensive method to expose adhering cultured cells to ozonization. Microscopie, 23(1), 46–52. https://doi.org/10.4081/microscopie.2015.5164

PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.

An Open Access Publication is one that meets the following two conditions:

  1. the author(s) and copyright holder(s) grant(s) to all users a free, irrevocable, worldwide, perpetual right of access to, and a license to copy, use, distribute, transmit and display the work publicly and to make and distribute derivative works, in any digital medium for any responsible purpose, subject to proper attribution of authorship, as well as the right to make small numbers of printed copies for their personal use.
  2. a complete version of the work and all supplemental materials, including a copy of the permission as stated above, in a suitable standard electronic format is deposited immediately upon initial publication in at least one online repository that is supported by an academic institution, scholarly society, government agency, or other well-established organization that seeks to enable open access, unrestricted distribution, interoperability, and long-term archiving.

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.

Downloads

Download data is not yet available.

Citations

Crossref
Scopus
Google Scholar
Europe PMC