Melatonin prevents hydrogen peroxide-induced apoptotic cell death


https://doi.org/10.4081/microscopie.2010.4970
Vol. 13 No. 1 (2010)
Submitted: 15 January 2015
Accepted: 15 January 2015
Published: 31 March 2010
U937, melatonin, hydrogen peroxide, apoptosis, reactive oxygen species.
Abstract Views: 462
PDF: 1061
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Authors

  • S. Salucci
    sara.salucci@uniurb.it
    DISUAN, Università degli Studi di Urbino “Carlo Bo”, Urbino, Italy.
  • S. Burattini DISUAN, Università degli Studi di Urbino “Carlo Bo”, Urbino, Italy.
  • M. Battistelli DISUAN, Università degli Studi di Urbino “Carlo Bo”, Urbino, Italy.
  • F. Chiarini Dipartimento di Scienze Anatomiche Umane e Fisiopatologia dell’Apparato Locomotore, Università di Bologna, Bologna, Italy.
  • A.M. Martelli Dipartimento di Scienze Anatomiche Umane e Fisiopatologia dell’Apparato Locomotore, Università di Bologna, Bologna; Istituto di Genetica Molecolare, CNR, Istituti Ortopedici Rizzoli, Bologna, Italy.
  • P. Sestili Dipartimento di Scienze Biomolecolari, IRAM; Università degli Studi di Urbino “Carlo Bo”, Urbino, Italy.
  • A. Valmori Istituto di Genetica Molecolare, CNR, Istituti Ortopedici Rizzoli, Bologna, Italy.
  • P. Gobbi DISUAN, Università degli Studi di Urbino “Carlo Bo”, Urbino, Italy.
  • E. Falcieri DISUAN, Università degli Studi di Urbino “Carlo Bo”, Urbino; Istituto di Genetica Molecolare, CNR, Istituti Ortopedici Rizzoli, Bologna, Italy.
Melatonin (MEL) functions in organisms are diverse. The actions considered in the current work relate to its ability to prevent oxidative stress, i.e. molecular damage produced by reactive oxygen species (ROS). Apoptosis is an active form of cell death that is initiated by a variety of stimuli, some of which inducing ROS increase. Hydrogen peroxide (H2O2) is considered a typical cytotoxic and oxidant agent capable to induce cellular damage through free radical production. In the present work we have investigated its role in the induction of cell death. U937 cells were exposed to 500 μM H2O2 and MEL behaviour, in the presence of this type of oxidative stress, was evaluated by incubating cells with MEL before and after H2O2 exposure. Cytometric and microscopy analyses were utilized and revealed that H2O2 can be considered an apoptotic trigger, if used at proper concentrations. In fact U937 cells, after 500 μM H2O2 exposure, showed chromatin condensation, micronuclei presence, apoptotic bodies and secondary necrosis. MEL, added before H2O2 treatment, significantly reduced apoptotic cell number. On the contrary, MEL incubation after H2O2 induced an increase of apoptotic cell death, and cells in secondary necrosis were detectable. These results indicated that pre-incubation with MEL reduces H2O2-dependent apoptosis in U937 cells, suggesting a capacity of this hormone to interfere with apoptosis induced by ROS increase.

Salucci, S., Burattini, S., Battistelli, M., Chiarini, F., Martelli, A., Sestili, P., Valmori, A., Gobbi, P., & Falcieri, E. (2010). Melatonin prevents hydrogen peroxide-induced apoptotic cell death. Microscopie, 13(1), 51–57. https://doi.org/10.4081/microscopie.2010.4970

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