https://doi.org/10.4081/jsas.2011.564

QUERCETIN AND RUTIN: EFFECTS OF TWO FLAVONOIDS ON INDUCED OXIDATIVE STRESS IN HUMAN EJACULATED SPERM

Vol. 3 No. 1 (2011)
Submitted: 20 August 2012
Accepted: 20 August 2012
Published: 20 August 2012
human sperm, lipid peroxidation, quercetin, rutin, TEM
Abstract Views: 2670
PDF: 1311
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Authors

L. Mazzi
Department of Biomedical Sciences, Applied Biology Section, University of Siena, Italy.
M. Geminiani
Department of Biomedical Sciences, Applied Biology Section, University of Siena, Italy.
G. Collodel
Department of Biomedical Sciences, Applied Biology Section; Interdepartmental Centre for Research and Therapy of Male Infertility, University of Siena, Italy.
F. Iacoponi
Department of Biomedical Sciences, Applied Biology Section, University of Siena, Italy.
S. Martini
Department of Pharmaceutical and Applied Chemistry, University of Siena; Centre for Colloid and Surface Science (CSGI), University of Florence, Italy.
C. Bonechi
Department of Pharmaceutical and Applied Chemistry, University of Siena; Centre for Colloid and Surface Science (CSGI), University of Florence, Italy.
C. Rossi
Department of Pharmaceutical and Applied Chemistry, University of Siena; Centre for Colloid and Surface Science (CSGI), University of Florence, Italy.
E. Moretti
elena.moretti@unisi.it
Department of Biomedical Sciences, Applied Biology Section; Interdepartmental Centre for Research and Therapy of Male Infertility, University of Siena, Italy.
Quercetin (Q) and rutin (R) are two natural flavonoids with antioxidant properties. We evaluated the toxicity of Q and R at 20μM, 30μM, 50μM, 100μM, 200μM, 400μM in swim up selected human sperm. The antioxidant activity (Q and R 20μM and 30μM) was tested on lipid peroxidation (LPO) induced by tert-butylhydroperoxide in human sperm. LPO was evaluated using the C11-BODIPY581/591 probe and sperm structural damages were assessed by transmission electron mi- croscopy in samples incubated with and without Q and R. A significant dose dependent toxic effect was observed for both compounds on sperm viability (Q and R: r= -0.98 P<0.001), on sperm progressive motility (Q: r=-0.98, R: r=-0.97; P<0.001), and on non progressive motility (Q; r=-0.58, P<0.01; R: r=-0.50, P<0.05). Both flavonoids, used at 20μM and 30μM, showed antioxidant properties on LPO induced in human sperm and a general protective effect against ultrastructural damages of LPO. In conclusion, we observed that Q exhibited a little higher toxicity than R; on the other hand R is little low protective on induced LPO. Our preliminary results demonstrated the scavenger properties of these flavonoids in vitro on human sperm.

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How to Cite

Mazzi, L., Geminiani, M., Collodel, G., Iacoponi, F., Martini, S., Bonechi, C., Rossi, C., & Moretti, E. (2012). QUERCETIN AND RUTIN: EFFECTS OF TWO FLAVONOIDS ON INDUCED OXIDATIVE STRESS IN HUMAN EJACULATED SPERM. Journal of the Siena Academy of Sciences, 3(1), 22–26. https://doi.org/10.4081/jsas.2011.564

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