Protease of Stenotrophomonas sp. from Indonesian fermented food: gene cloning and analysis

Submitted: December 5, 2016
Accepted: June 3, 2017
Published: February 23, 2018
Abstract Views: 1225
PDF: 535
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Screening of proteolytic and fibrinolytic bacteria from Indonesian soy bean based fermented food Oncom revealed several potential isolates. Based on 16s rDNA gene analysis, one particular isolate with the highest proteolytic and fibrinolytic activity was identified as Stenotrophomonas sp. The protease gene was amplified to generate a 1749 bp Polymerase Chain Reaction product and BLAST analysis, revealed 90% homology with gene encoding protease enzyme from Stenotrophomonas maltophilia. The putative amino acid sequence indicated a serine protease enzyme with typical amino acid aspartate, histidine and serine in the catalytic triad. The gene was translated into a pre-pro-protein consisted of cleavage site on its N terminal and Pre-Peptidase Cterminal domain. Cloning of the protease gene in pET22b with Escherichia coli BL21 DE3 as the host showed that the gene was expressed as insoluble protein fraction. This is the first report for analysis of protease gene from food origin Stenotrophomonas sp.

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Supporting Agencies

Faculty of Biotechnology, Atma Jaya Catholic University, DLBS Dexa Medica,

How to Cite

Kurnia, F., Tjandrawinata, R. R., Yulandi, A., & Suhartono, M. T. (2018). Protease of Stenotrophomonas sp. from Indonesian fermented food: gene cloning and analysis. Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 90(2). https://doi.org/10.4081/jbr.2017.6428