https://doi.org/10.4081/jbr.2016.6211

Haemostatic property of Chromolaena odorata leaf extracts: in vitro and in vivo evaluation in wistar rats

Vol. 89 No. 2 (2016)
Submitted: July 29, 2016
Accepted: November 3, 2016
Published: December 20, 2016
Chromolaena odorata, Haemostatic activity, Haemostasis
Abstract Views: 2209
PDF: 1129
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Authors

Henshaw Uchechi Okoroiwu
okoroiwuhenshaw@gmail.com
Department of Medical Laboratory Science, University of Calabar, Calabar, Nigeria.
Item Justin Atangwho
Department of Biochemistry, University of Calabar, Calabar, Nigeria.
Emmanuel Kufre Uko
Department of Medical Laboratory Science, University of Calabar, Calabar, Nigeria.
Okafor Ifeyinwa Maryann
Department of Medical Laboratory Science, University of Calabar, Calabar, Nigeria.
This study was designed to investigate the effects of aqueous, ethanol and crude extracts of Chromolaena odorata leaf on haemostatic mechanism of wistar rats and its possible in vitro use in coagulation study. Fifty wistar rats of both sexes weighing between 140-180 g were sorted into 10 groups each fed via oral gavage once daily for 21 days. Sample collection was done by cardiac puncture. Bleeding and clotting times were performed using Duke’s and Ivy’s methods, respectively. The prothrombin time was performed using the Quick’s one stage method, while the partial thromboplastin time using kaolin was done using Macpherson and Hardity method using Giess diagnosis reagent. Significant results were observed in the bleeding and clotting times of the three extracts in a dose-dependent manner. The 300-mg/kg ethanol extract decreased the bleeding time more than the other two extracts. Only the ethanol 150 and 300 mg/mL showed in vitro activity. The study showed the in vivo haemostatic properties of Chromolaena odorata leaf extracts and its possible use in in vitro coagulation study.

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Supporting Agencies

research was sponsored by authors
Henshaw Uchechi Okoroiwu, Department of Medical Laboratory Science, University of Calabar, Calabar
Ph.D student Haematology unit, department of medical laboratory science, university of calabar, nigeria
Item Justin Atangwho, Department of Biochemistry, University of Calabar, Calabar
Professor, Haematology unit, department of medical laboratory science, university of calabar, nigeria
Emmanuel Kufre Uko, Department of Medical Laboratory Science, University of Calabar, Calabar
Lecturer 1, Haematology unit, department of medical laboratory science, university of calabar, nigeria
Okafor Ifeyinwa Maryann, Department of Medical Laboratory Science, University of Calabar, Calabar
senior lecturer, department of biochemistry, university of calabar

How to Cite

Okoroiwu, H. U., Atangwho, I. J., Uko, E. K., & Maryann, O. I. (2016). Haemostatic property of Chromolaena odorata leaf extracts: in vitro and in vivo evaluation in wistar rats. Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 89(2). https://doi.org/10.4081/jbr.2016.6211

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