https://doi.org/10.4081/jbr.2009.4734

Cytotoxic effect on human keratinocytes of crude extracts from planktonic Cnidaria

Vol. 82 No. 1 (2009)
Submitted: September 30, 2014
Accepted: September 30, 2014
Published: January 30, 2009
Plankton, jellyfish, keratinocytes, cytotoxicity
Abstract Views: 1188
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Authors

A. Parodi
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A.M. Bassi
M. Catalano
E. Giacco
G.L. Mariottini
L. Pane
Biotoxin production is a characterizing aspect of the physiology and ecology of several marine organisms. In this study the cytotoxic effect of crude extracts from jellyfish Pelagia noctiluca, Chrysaora hysoscella and Aurelia aurita (Cnidaria: Scyphozoa) against cultured human keratinocytes NCTC 2544 was evaluated by 3- (4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) Assay and Neutral Red Uptake (NRU). Low cytotoxic effects were observed with NRU, while MTT showed a dose-dependent effect, with a "plateau" at highest protein concentrations. Therefore, crude extraxts didn't show effect on lysosomes but MTT test emphasized cell survival decrease and a "plateau" effect at high extract doses which could be related with the inhibition of the mitochondrial dehydrogenase activity.

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How to Cite

Parodi, A., Bassi, A., Catalano, M., Giacco, E., Mariottini, G., & Pane, L. (2009). Cytotoxic effect on human keratinocytes of crude extracts from planktonic Cnidaria. Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 82(1). https://doi.org/10.4081/jbr.2009.4734

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