Cardiac regeneration by pharmacologically active microcarriers releasing growth factors and/or transporting adipose-derived stem cells

Submitted: November 21, 2013
Accepted: November 21, 2013
Published: January 10, 2014
Abstract Views: 1608
PDF: 1226
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We tested the hypothesis that cardiac regeneration through local delivery of adipose-derived stem cells (ASCs), activation of resident cardiac stem cells via growth factors (GFs) [hepatocyte growth factor (HGF) and insulin-like growth factor 1 (IGF-1):GFs] or both, are improved by pharmacologically active microcarriers (PAMs) interacting with cells/molecules conveyed on their surface. Rats with one-month old myocardial infarction were treated with ASCs, ASCs+PAMs, GF-releasing PAMs, ASCs+GF-releasing PAMs or vehicle. Two weeks later, hemodynamic function and inducibility of ventricular arrhythmias (VAs) were assessed. Eventually, the hearts were subjected to anatomical and immunohistochemical analyses. A significant ASCs engraftment and the largest improvement in cardiac mechanics occurred in ASC+GF-releasing PAM rats which by contrast were more vulnerable to VAs. Thus, PAMs may improve cell/GF-based cardiac regeneration although caution should be paid on the electrophysiological impact of their physical interaction with the myocardium.

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Savi, M., Bocchi, L., Fiumana, E., Frati, C., Bonafé, F., Cavalli, S., Morselli, P. G., Karam, J.-P., Montero-Menei, C., Caldarera, C. M., Guarnieri, C., Muscari, C., Stilli, D., Quaini, F., & Musso, E. (2014). Cardiac regeneration by pharmacologically active microcarriers releasing growth factors and/or transporting adipose-derived stem cells. Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 87(1). https://doi.org/10.4081/jbr.2014.2141