https://doi.org/10.4081/jbr.2014.2140

New perspective in the assessment of total intracellular magnesium

Vol. 87 No. 1 (2014): Proceedings of the 85th SIBS Congress
Submitted: November 21, 2013
Accepted: November 21, 2013
Published: January 10, 2014
total intracellular magnesium, biological process, DCHQ
Abstract Views: 1026
PDF: 1198
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Authors

Azzurra Sargenti
azzurra.sargenti@unibo.it
Department of Pharmacy and Biotechnologies, University of Bologna, Italy.
Lucia Merolle
Department of Pharmacy and Biotechnologies, University of Bologna, Italy.
Giulia Andreani
Department of Veterinary Medical Sciences, University of Bologna, Italy.
Concettina Cappadone
Department of Pharmacy and Biotechnologies, University of Bologna, Italy.
Giovanna Farruggia
National Institute of Biostructures and Biosystems, Roma, Italy.
Stefano Iotti
National Institute of Biostructures and Biosystems, Roma, Italy.
Chiara Marraccini
Department of Life Sciences, University of Modena and Reggio Emilia, Italy.
Magnesium (Mg) is essential for biological processes, but its cellular homeostasis has not been thoroughly elucidated, mainly because of the inadequacy of the available techniques to map intracellular Mg distribution. Recently, particular interest has been raised by a new family of fluorescent probes, diaza-18-crown-hydroxyquinoline (DCHQ), that shows remarkably high affinity and specificity for Mg, thus permitting the detection of the total intracellular Mg. The data obtained by fluori- metric and cytofluorimetric assays performed with DCHQ5 are in good agreement with atomic absorption spectroscopy, confirming that DCHQ5 probe allows both qualitative and quantitative determination of total intracellular Mg.

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How to Cite

Sargenti, A., Merolle, L., Andreani, G., Cappadone, C., Farruggia, G., Iotti, S., & Marraccini, C. (2014). New perspective in the assessment of total intracellular magnesium. Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 87(1). https://doi.org/10.4081/jbr.2014.2140

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