https://doi.org/10.4081/jbr.2014.2139
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Intracellular magnesium content changes during mitochondria-mediated apoptosis: in depth study of early events on mitochondrial membrane potential

Authors

Lucia Merolle
lucia.merolle@unibo.it
Department of Pharmacy and Biotechnologies, University of Bologna, Italy.
Concettina Cappadone
Department of Pharmacy and Biotechnologies, University of Bologna, Italy.
Giovanna Farruggia
National Institute of Biostructures and Biosystems, Roma, Italy.
Chiara Marraccini
Department of Life Sciences, University of Modena and Reggio Emilia, Italy.
Azzurra Sargenti
Department of Pharmacy and Biotechnologies, University of Bologna, Italy.
Alessia Colanardi
Institute of Translational Pharmacology - CNR, L’Aquila, Italy.
Stefano Iotti
Department of Pharmacy and Biotechnologies, University of Bologna, Italy.
A recent study showed the antitumor activity of a new indole-derivative – MM-67 – inducing mitochondria-mediated apoptosis and a decrease of intracellular magnesium (Mg) concentration in HT29 colon cancer cells. Aim of this work was to assess cellular Mg levels throughout MM-67-induced apoptosis from the early to the final stage of the process and to evaluate the correlation with mitochondrial membrane potential (ΔΨm) variations. All analysis were performed by flow cytometry: ΔΨm was assessed by using mitochondrial potential sensitive dye DiOC6, while free and total intracellular cation concentrations were assessed by using the commercial probe MagFluo4-AM (Kd=4.7 mM), and the new synthesized DCHQ5 (Kd=8.3 mM), respectively. Our results evidenced that the MM67 induced apoptosis is characterized by a direct correlation between ΔΨ and free intracellular Mg content variations.

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How to Cite

Merolle, L., Cappadone, C., Farruggia, G., Marraccini, C., Sargenti, A., Colanardi, A., & Iotti, S. (2014). Intracellular magnesium content changes during mitochondria-mediated apoptosis: in depth study of early events on mitochondrial membrane potential. Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 87(1). https://doi.org/10.4081/jbr.2014.2139

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