https://doi.org/10.4081/jbr.2014.2125

Spontaneous electrical activity of guinea-pig sinoatrial cells under modulation of two different pacemaker mechanisms

Vol. 87 No. 1 (2014): Proceedings of the 85th SIBS Congress
Submitted: November 21, 2013
Accepted: November 21, 2013
Published: January 10, 2014
sinoatrial node, Ivabradine, Ryanodine, cardiac rhythm, cardiac action potential
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Authors

Francesca Cacciani
francesca.cacciani@unipr.it
Department of Biosciences, University of Parma, Italy.
Massimiliano Zaniboni
Department of Biosciences, University of Parma, .
The main cellular determinants of cardiac automaticity are the hyperpolarization-activated cationic current If, and the electrogenic Na+/Ca2+ exchanger which generates an inward current after each action potential (AP). Our goal was to evaluate their relative role in pacemaking, by means of application of Ivabradine (IVA) (specific If blocker) and Ryanodine (RYA) (known to abolish calcium transient) on enzimatically isolated guinea-pig pacemaker cells. Spontaneous APs were recorded in patch-clamp whole cell configuration at 36°C from 7 cells perfused with the following sequence of solutions: physiological normal tyrode (NT), IVA 3 mM, NT and RYA 3 mM. Cycle length (CL, ms) and diastolic depolarization rate (DDR, V/s) were also calculated. Both blockers displayed similar effects, increasing CL (by 27 and 30%, respectively), and decreasing DDR (by 34 and 42%) with respect to NT exposure. These results suggest that both mechanisms are involved into pacemaking mechanism at a similar degree.

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How to Cite

Cacciani, F., & Zaniboni, M. (2014). Spontaneous electrical activity of guinea-pig sinoatrial cells under modulation of two different pacemaker mechanisms. Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 87(1). https://doi.org/10.4081/jbr.2014.2125

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