Phytochemical characterization and evaluation of the antibacterial potential of <i>Solenostemma oleifolium</i> (Nectoux) Bullock & E.A. Bruce ex Maire essential oil

Daouia Keltoum Benmaarouf; Bernard China; Baya Djallout; Soumiya Boulahlib; Hamza Aliboudhar; Diana C.G.A. Pinto; Safia Zenia; Meriem Hind Ben-Mahdi

doi:10.4081/jbr.2024.12321

Authors

Daouia Keltoum Benmaarouf Unit for evaluating the efficacy of pharmacological molecules and developing alternative strategies, Animal Health and Production Research Laboratory, Ecole Nationale Supérieure Vétérinaire, Algiers, Algeria.
Bernard China
bernard.china@sciensano.be
Sciensano, Belgian Institute of Health, Brussels, Belgium. https://orcid.org/0000-0001-5717-478X
Baya Djallout Unit for evaluating the efficacy of pharmacological molecules and developing alternative strategies, Animal Health and Production Research Laboratory, Ecole Nationale Supérieure Vétérinaire, Algiers, Algeria.
Soumiya Boulahlib Organic Functional Analysis Laboratory, Faculty of Chemistry, University of Science and Technology Houari Boumediene, El Alia, Bab Ezzouar, Algiers, Algeria. https://orcid.org/0000-0001-7650-7199
Hamza Aliboudhar Organic Functional Analysis Laboratory, Faculty of Chemistry, University of Science and Technology Houari Boumediene, El Alia, Bab Ezzouar, Algiers, Algeria. https://orcid.org/0000-0001-8761-1362
Diana C.G.A. Pinto LAQV-REQUIMTE and Department of Chemistry, University of Aveiro, Campus de Santiago, Aveiro, Portugal.
Safia Zenia Unit for evaluating the efficacy of pharmacological molecules and developing alternative strategies, Animal Health and Production Research Laboratory, Ecole Nationale Supérieure Vétérinaire, Algiers, Algeria. https://orcid.org/0000-0002-2319-1001
Meriem Hind Ben-Mahdi Unit for evaluating the efficacy of pharmacological molecules and developing alternative strategies, Animal Health and Production Research Laboratory, Ecole Nationale Supérieure Vétérinaire, Algiers, Algeria.

Solenostemma oleifolium is a tropical plant growing in the Algerian desert that is traditionally used to treat several illnesses, including infection. We investigate essential oil components from leaves of Solenostemma oleifolium (EOSO) and its antibacterial activity. Using Gas Chromatography and Mass Spectrometry (GC-MS), twenty compounds were identified in EOSO, including linalool (57.10%), terpineol (12.95%), trans-geraniol (12.65%), and nerol (4.67%). Nuclear magnetic resonance (NMR) analysis allowed us to confirm linalool as the main component of EOSO. Antibacterial activity was tested by agar diffusion and microdilution methods for minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). For EOSO, the inhibition diameters ranged from 16.79 to 39.84 mm, the MIC ranged from 1.066 to 8.54 mg mL^-1and the MBC ranged from 20 to 100 mg mL^-1. For linanool, the inhibition diameters ranged from 11.1 to 31.87 mm, the MIC ranged from 2.68 to 14.3 mg mL^-1 and, the MBC ranged from 40 to 100 mg mL^-1.EOSO and linalool exhibited significant antibacterial activity against all the tested bacteria. This study confirmed the antibacterial activity of the S. oleifolium essential oil and that linalool is the principal constituent of the EOSO.

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Benmaarouf, D. K., China, B., Djallout, B., Boulahlib, S., Aliboudhar, H., Pinto, D. C., Zenia, S., & Ben-Mahdi, M. H. (2024). Phytochemical characterization and evaluation of the antibacterial potential of <i>Solenostemma oleifolium</i> (Nectoux) Bullock & E.A. Bruce ex Maire essential oil . Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 97(1). https://doi.org/10.4081/jbr.2024.12321