https://doi.org/10.4081/jbr.2022.10820

pH evaluation of storage fluids and ancient DNA extraction from wet specimens in pathology museums

Vol. 95 No. 2 (2022)
Submitted: August 29, 2022
Accepted: September 20, 2022
Published: October 7, 2022
Pathology museums, paleopathology, wet specimens, storage fluids, ancient DNA
Abstract Views: 973
PDF: 261
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

Luisa Ferrari
Division of Pathology, Cardinal Massaja Hospital, Asti; Department of Oncology, Pathology Unit, University of Turin, Italy.
Jasna Metovic
Department of Oncology, Pathology Unit, University of Turin, Italy.
Daniela Pulcinelli
Regional Centre of Immunohematology and Tissue Typing, San Salvatore Hospital, L’Aquila, Italy.
Antonio Lombardi
Regional Centre of Immunohematology and Tissue Typing, San Salvatore Hospital, L’Aquila, Italy.
Daniela Rullo
Division of Pathology, San Salvatore Hospital, L’Aquila, Italy.
Franco Papola
Regional Centre of Immunohematology and Tissue Typing, San Salvatore Hospital, L’Aquila, Italy.
Luca Ventura
lventura@asl1abruzzo.it
Division of Pathology, San Salvatore Hospital, L’Aquila; Department of Biotechnological and Applied Clinical Sciences, University of L’Aquila, Italy.

Pathology museums host ancient samples obtained during autopsies and generally used for educational purposes in the past. Such collections consist of dry and wet specimens showing diseases that no longer exist or with their natural course unmodified by modern therapies.1,2 In wet specimens, the preservation of macroscopic features due to the storage fluid has a great historical and paleopathological interest. Unfortunately, both original fixatives and storage fluids strongly influence tissue antigens and nucleic acids preservation.3 [...]

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

Citations

Crossref
Scopus
Google Scholar
Europe PMC
Ferrari L, Coda R, Fulcheri E, Bussolati G. The role of the pathological anatomy museum: Past glory, present crisis, and future prospects. Pathologica 2001;93:196-200.
Ferrari L. From body preservation to pathology museums in Italy: Conservation and modern value of a historical and biological archive. Med Secoli 2021;33:441-82.
Fulcheri E, Micalizio S, Ferrari L. Valore museale delle soluzioni di dimora nelle preparazioni anatomiche umane. Museol Sci 2008;3:88-92.
Schäffer S, Zachos FE, Koblmueller S. Opening the treasure chest: A DNA-barcoding primer set for most higher taxa of Central European birds and mammals from museum collections. PLOS One 2017;12:e0174449.
Strauben N, Lyra ML, Paijmans JLA, et al. Successful application of ancient DNA extraction and library construction protocols to museums wet collection specimens. Mol EcolResour 2021;21:2299-315.
Ferrari L, Metovic J, Rullo D, et al. Histopathology and DNA evaluation of wet specimens from the Pathology Collection of Turin. Virchows Archiv 2019;475:S35.
Hühns M, Röpenack P, Erbersdobler A. Molecular and immunohistochemical characterization of historical long-term preserved fixed tissues from different human organs. PLOS One 2015;10:e0135297.
Hühns M, Erbersdobler A, Obliers A, Röpenack P. Identification of HPV types and Mycobacterium tuberculosis complex in historical long-term preserved formalin fixed tissues in different human organs. PLOS One 2017;12:e0170353.
Hargrave CW, Sutherland KG, Marsh-Matthews E, Matthews WJ. Multiple interacting factors affect pH in museum storage solutions. Collection Forum 2005;19:23-31.

How to Cite

Ferrari, L., Metovic, J., Pulcinelli, D., Lombardi, A., Rullo, D., Papola, F., & Ventura, L. (2022). pH evaluation of storage fluids and ancient DNA extraction from wet specimens in pathology museums. Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 95(2). https://doi.org/10.4081/jbr.2022.10820
Copyright (c) 2022 the Authors Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.