https://doi.org/10.4081/jbr.2022.10474
Glycine betaine enhancing plant growth and antioxidant activity of fenugreek (Trigonella foenum-graecum L.) under salt stress
Submitted: March 22, 2022
Accepted: August 28, 2022
Published: September 2, 2022
Accepted: August 28, 2022
Abstract Views: 994
PDF: 314
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
The effect of an exogenous foliar spray of Glycine Betaine (GB) on vegetative growth and some biochemical aspects of fenugreek (Trigonella foenum-graecum L.) in the presence of salt was studied in a pot experiment. In addition to the control, four GB concentrations (0, 25, 50, and 75 mM) were used under salt concentration, 150 mM NaCl. Salt stress reduced all growth parameters and had a negative impact on photosynthetic pigments, relative water content, and proteins. The foliar spray of GB, on the other hand, raises these parameters, particularly at concentrations of 50 mM. Carbohydrates, total phenolics, flavonoids, and antioxidant activity all increased in response to salt stress compared to controls; additionally, GB foliar spray induced their accumulation. Furthermore, increasing the concentration of GB increased these parameters. GB, more than others, reduces the entry of harmful ions (Na+ and Cl-), while increasing the accumulation of Ca2+ and K+. These findings suggest that foliar spraying with GB can improve fenugreek growth and tolerance under saline conditions.
Petropoulos GA. Fenugreek—The Genus Trigonella. 1st ed. Taylor and Francis: London, UK; New York, NY, USA; 2002. pp 1–127.
Abd Elhamid EM, Sadak MS, Tawfik MM. Physiological response of fenugreek plant to the application of proline under different water regimes. Res J Pharma Biol Chem Sci 2016;7:580–94.
Abdel Latef AA, Abu Alhmad MF, Ahmad S. Foliar application of fresh moringa leaf extract overcomes salt stress in fenugreek (Trigonella foenum-graecum) plants. Egy J Bot 2017;57:157–79.
Seleiman MF, Semida WM, Rady MM, et al. Sequential application of antioxidants rectifies ion imbalance and strengthens antioxidant systems in salt-stressed cucumber. Plants 2020;9:1783.
Radi R. Oxygen radicals, nitric oxide, and peroxynitrite: Redox pathways in molecular medicine. Proc Nat Acad Sci, USA 2018;115:5839−48.
Flowers TJ, Colmer TD. Salinity tolerance in halophytes. New Phytol 2008;179:945−63.
Patrick DJ. Plant biostimulants: Definition, concept, main categories and regulation. Sci Hortic 2015;196:3–14.
Malekzadeh P. Influence of exogenous application of glycine betaine on antioxidative system and growth of salt stressed soybean seedlings (Glycine max L.). Plant Physiol Mol Biol 2015;21:225−32.
Hamani AK, Li S, Chen J, et al. Linking exogenous foliar application of glycine betaine and stomatal characteristics with salinity stress tolerance in cotton (Gossypium hirsutum L.) seedlings. BMC Plant Biol 2021;21:146.
Hoque MA, Banu MN, Okuma E, et al. Exogenous proline and glycine betaine increase NaCl- induced ascorbate-glutathione cycle enzyme activities, and proline improves salt tolerance more than glycine betaine in tobacco bright Yellow-2 suspension-cultured cells. J Plant Physiol 2007;164:1457–68.
Alasvandyari F, Mahdavi B, Hosseini S. Glycine betaine affects the antioxidant system and ion accumulation and reduces salinity-induced damage in safflower seedlings. Arch Biol Sci 2017;69:139−47.
Carter MR, Gregorich EG. Soil sampling and methods of analysis. 2nd ed. CRC Press-Taylor & Francis Group, Boca Raton, Florida, USA; 2008.
Hsu YT, Kao CH. Role of abscisic acid in cadmium tolerance of rice (Oryza sativa L.) seedlings. Plant Cell Environ 2003;26:867−74.
Moran R. Formula for determination of chlorophyllous pigments extracted with N.N. dimethylformamide. Plant Physiol 1982;69:1371–81.
Mostofa MG, Fujita M. Salicylic acid alleviates copper toxicity in rice seedlings by up-regulating antioxidative and glyoxalase systems. Ecotoxicol 2013;22:959−73.
Bradford MM. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Annu Rev Biochem 1976;72:248−54.
Dubois M, Gilles KA, Hamilton JK, et al. Calorimetric method for determination of sugars and related substances. Anal Chem 1956;28:350−6.
Jindal KK, Singh RN. Phenolic content in male and female Carica papaya: A possible physiological marker sex identification of vegetative seedlings. Physiol Plant 1975;33:104–7.
Ordoñez AA, Gomez JD, Vattuone MA, Lsla MI. Antioxidant activities of Sechium edule (Jacq.) Swartz extracts. Food Chem 2006;97:452–8.
Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci Technol 1995;28:25–30.
Marschner P. MARSCHNER’S mineral nutrition of higher plants. 3rd ed. Elsevier: London; 2011.
Athar H, Zafar ZU, Ashraf M. Glycine betaine improved photosynthesis in canola under salt stress: Evaluation of chlorophyll fluorescence parameters as potential indicators. J Agron Crop Sci 2015;201:428–42.
Yildirim E, Ekinci M, Turan M, et al. Roles of glycine betaine in mitigating deleterious effect of salt stress on lettuce (Lactuca sativa L.). Arch Agric Soil Sci 2015;61:1673–89.
Murata N, Chen TH. Glycinebetaine protects plants against abiotic stress: mechanisms and biotechnological applications. Plant Cell Environ 2011;34:1–20.
García-Caparrós P, Lianderal A, Hegarat E, et al. Effects of exogenous application of osmotic adjustment substances on growth, pigment concentration, and physiological parameters of Dracaena sanderiana Sander under different levels of salinity. Agronomy 2020;10:125.
Mäkela P, Mantila J, Hinkkanen R, et al. Effect of foliar applications of glycine betaine on stress tolerance, growth and yield of spring cereals and summer turnip rape in Finland. J Agron Crop Sci 1996;176:223–34.
Abbas W, Ashraf M, Akrama NA. Alleviation of salt-induced adverse effects in eggplant (Solanum melongena L.) by glycine betaine and sugar beet extracts. Sci Hortic 2010;125:188–95.
Habib N, Ashraf M, Ali Q, Perveen R. Response of salt stressed okra (Abelmoschus esculentus Moench) plants to foliar-applied glycine betaine and glycine betaine containing sugar beet extract. South Afric J Bot 2012;83:151–8.
Rahneshan Z, Nasibi F, Moghadam AA. Effects of salinity stress on some growth, physiological, biochemical parameters and nutrients in two pistachio (Pistacia vera L.) rootstocks. J Plant Interact 2018;13:73−82.
Melgar JC, Benlloch M, Fernández-Escobar R. Calcium increases sodium exclusion in olive plants. Sci Hortic 2006;109:303−5.
Abdel-Fattah GM, Rabie GH, Lamis DS, Metwally RA. The impact of arbuscular mycorrhizal fungi on growth and physiological parameters of cowpea plants grown under salt stress conditions. Inter J Appl Sci Biotechnol 2016;4:372–9.
Shi Q, Bao Z, Zhu Z, et al. Effects of different treatments of salicylic acid on heat tolerance, chlorophyll fluorescence and antioxidant enzyme activity in seedlings of Cucumis sativa L. J Plant Growth Regul 2006;48:127–35.
Dustgeer Z, Seleiman MF, Khan I, et al. Glycine-betaine induced salinity tolerance in maize by regulating the physiological attributes, antioxidant defense system and ionic homeostasis. Not Bot Horti Agrobot Cluj Napoca 2021;49:12248.
Golldack D, Quigley F, Michalowski CB, et al. Salinity stress tolerant and sensitive rice (Oryza sativa L.) regulate AKT1-type potassium channel transcripts differently. Plant Mol Biol 2003;51:71−81.
Blum A. Osmotic adjustment is a prime drought stress adaptive engine in support of plant production. Plant Cell Environ 2017;40:4−10.
Khalifa GS, Abdelrassoul M, Hegazi AM, Elsherif MH. Attenuation of negative effects of saline stress in two lettuce cultivars by salicylic acid and glycine betaine. Gesunde Pflanz 2016;68:177–89.
Manaf HH. Beneficial effects of exogenous selenium, glycine betaine and seaweed extract on salt stressed cowpea plant. Annu Agric Sci 2016;61:41–8.
El-Ramady H, Alshaal T, Elhawat N, et al. Plant nutrients and their roles under saline soil conditions, In: Hasanuzzaman M, Plant nutrients and abiotic stress tolerance. Springer Nature Singapore Pte Ltd; 2018. pp 297-324.
Abdelhameed RE, Abdel Latef AA, Shehata RS. Physiological responses of salinized fenugreek (Trigonella foenum-graecum L.) plants to foliar application of salicylic acid. Plants 2021;10:657.
Taiz L, Zeiger E, Moller IM, Murphy A. Abiotic stress, in: Plant Physiology. 6th ed. Sunderland, MA Sinauer Assoc Inc; 2015. pp 730–1.
How to Cite
Ismaiel, S. A., & El-Bialy, N. M. . (2022). Glycine betaine enhancing plant growth and antioxidant activity of fenugreek (<em>Trigonella foenum-graecum</em> L.) under salt stress. Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 95(2). https://doi.org/10.4081/jbr.2022.10474
Copyright (c) 2022 the Authors
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.