https://doi.org/10.4081/jbr.2022.10299

Antioxidant and antifungal activities in vitro of essential oils and extracts of twelve Algerian species of Thymus against some mycotoxigenic Aspergillus genera

Vol. 95 No. 1 (2022)
Submitted: November 25, 2021
Accepted: December 28, 2021
Published: January 11, 2022
Thymus spp., essential oil, extracts, antioxidant activity, antifungal activity
Abstract Views: 2288
PDF: 556
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Authors

Yamina Ben Miri
yamina.benmiri@univ-msila.dz
Department of Biochemistry and Microbiology, Faculty of Sciences, Mohamed Boudiaf University, M’sila; Food Quality and Safety Research Laboratory, Department of Food Sciences, Mouloud Mammeri University, Tizi-Ouzou, Algeria.
Aldjia Taoudiat
Department of Agronomy, Faculty of Life and Nature Sciences, Akli Mohand Oulhadj University, Bouira, Algeria.
Mohamed Mahdid
Laboratory of Ethnobotany and Natural Substances, Department of Natural Science, Ecole Normale Supérieure (ENS), Vieux Kouba, Algiers, Algeria.

The aim of the study was to determine the phenolic and flavonoid content of Essential Oils (EOs), chloroform and ethanolic extracts of 12 Algerian Thymus species and evaluate their antioxidant and antifungal activities. EOs (1.73±0.30– 15.00±1.24μg/mg), chloroform extracts (33.8±2.42– 160.93±3.88μg/mg) and ethanol extracts (27.01±3.56 – 148.46±4.40μg/mg) showed considerable phenolic content. Flavonoids values of chloroform extracts ranged between 3.39±0.17 and 20.27±0.29μg/mL while ethanolic extracts values ranged between 2.81±0.11 and 26.64±0.18μg/mg. Results of DPPH showed that EOs, chloroform and ethanolic extracts exhibited strong radical scavenging activity (IC50=21.75±6.54– 338.22±2.99μg/mL, 22.91±5.59–90.93±1.36μg/mL, and 33.51±5.72–103.80±4.54μg/m, respectively). Inhibition of β-carotene bleaching was potentially performed by all EOs (66.48±2.41–94.06±2.68%), chloroform extracts (68.98±1.58– 95.30±1.99%), and ethanolic extracts (62.15±2.51–92.36±1.15%). The antifungal activity of EOs and extracts was tested using the Minimum Inhibitory Concentration (MIC) and minimum fungicidal concentration (MFC). The EOs (0.1±0.00mg/mL– 1.06±0.46mg/mL), chloroform (0.1±0.00 mg/mL– 1.06±0.46mg/mL) and ethanol (0.1±0.00mg/mL–1.6±0.00 mg/mL) showed remarkable antifungal activity against mycotoxigenic Aspergillus genera. The MFC of EOs (1.0±0.34mg/mL and >4.8mg/mL), chloroform (0.26±0.11mg/mL and >1.6 mg/mL) and ethanol (0.2±0.00mg/mL and >1.6 mg/mL) were fungicidal in nature higher than MICs. The findings of the study indicated that Thymus spp. EOs and extracts could be used as natural alternatives for food industry

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How to Cite

Ben Miri, Y., Taoudiat, A. ., & Mahdid, M. . (2022). Antioxidant and antifungal activities <em>in vitro</em> of essential oils and extracts of twelve Algerian species of <em>Thymus</em> against some mycotoxigenic <em>Aspergillus</em> genera. Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 95(1). https://doi.org/10.4081/jbr.2022.10299

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