Effects of chronic exposure to benzophenone and diclofenac on DNA methylation levels and reproductive success in a marine copepod

  • Anais Guyon Cawthron Institute, Nelson, New Zealand; European Institute for Marine Studies, University of Western Brittany, Plouzané, France.
  • Kirsty F. Smith Cawthron Institute, Nelson, New Zealand.
  • Maria P. Charry Cawthron Institute, Nelson; School of Biological Sciences, University of Auckland, Auckland, New Zealand.
  • Olivier Champeau Cawthron Institute, Nelson, New Zealand.
  • Louis A. Tremblay | louis.tremblay@cawthron.org.nz Cawthron Institute, Nelson; School of Biological Sciences, University of Auckland, Auckland, New Zealand. http://orcid.org/0000-0002-3586-3995

Abstract

The UV-filter benzophenone and the anti-inflammatory diclofenac are commonly detected in the environment. The aim of this study was to assess the multigenerational effects of chronic exposure to low concentrations of these chemicals on toxicity and DNA methylation levels in the copepod Gladioferens pectinatus. Acute toxicity tests were conducted to determine the sensitivity of G. pectinatus to the chemicals. All chemicals impacted breeding, hatching and egg viability. Diclofenac (1 mg.L-1) reduced the number of eggs per gravid female. Benzophenone (0.5 mg.L-1) decreased egg hatching success. Exposure to the reference toxicant copper (0.02 mg.L-1) led to unsuccessful hatching. Effects on DNA methylation was estimated by the percentage of 5- methylcytosine. The treatments resulted in strong differences in DNA methylation with increased methylation in the exposed animals. The two chemicals impacted both egg viability and the induction of differential DNA methylation, suggesting potential intra- and trans-generational evolutionary effects.

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Published
2018-10-29
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Issue
Section
Conference Presentations
Supporting Agencies
Centre for Integrated Biowaste Research, Institute of Environment Science and Research (ESR) Limited Strategic Science Investment Fund (SSIF), NZ Ministry of Business, Innovation and Employment, Boffa Miskell Limited, NZ
Keywords:
Gladioferens pectinatus, Toxicity, Multigenerational effects, Reproduction, Epigenetic, Emerging contaminants
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How to Cite
Guyon, A., Smith, K. F., Charry, M. P., Champeau, O., & Tremblay, L. A. (2018). Effects of chronic exposure to benzophenone and diclofenac on DNA methylation levels and reproductive success in a marine copepod. Journal of Xenobiotics, 8(1). https://doi.org/10.4081/xeno.2018.7674