Ameliorative effect of gallic acid on methotrexate-induced hepatotoxicity and nephrotoxicity in rat

  • Ebenezer Tunde Olayinka Biochemistry Unit, Department of Chemical Sciences, Ajayi Crowther University, Oyo, Oyo State, Nigeria.
  • Ayokanmi Ore | oreayokanmi@gmail.com Biochemistry Unit, Department of Chemical Sciences, Ajayi Crowther University, Oyo, Oyo State, Nigeria. http://orcid.org/0000-0003-1314-9325
  • Oluwatobi Adewumi Adeyemo Biochemistry Unit, Department of Chemical Sciences, Ajayi Crowther University, Oyo, Oyo State, Nigeria.
  • Olaniyi Solomon Ola Biochemistry Unit, Department of Chemical Sciences, Ajayi Crowther University, Oyo, Oyo State, Nigeria.

Abstract

We investigated the protective effect of gallic acid (GA) against methotrexate (MTX)-induced hepatotoxicity and nephrotoxicity. Male Wistar rats were randomized into five groups (n = 6/group): I, control; II, MTX-treated for seven days; III, pre-treated with GA for seven days, followed by MTX for seven days; IV, co-treated with MTX and GA for seven days and V, GA for seven days. MTX caused a significant increase (P<0.05) in plasma biomarkers of nephrotoxicity (urea, creatinine) and hepatotoxicity (Bilirubin, alkaline phosphatase, aspartate aminotransferase, alanine aminotransferase, gamma glutamyl transferase) when compared with control. Furthermore, MTX caused a significant decrease in the activities of hepatic enzymic antioxidants (superoxide dismutase, catalase, glutathione S-transferase) and nonenzymic antioxidants (Vitamin C and glutathione), followed by a significant increase in hepatic malondialdehyde content. However, pretreatment and co-treatment with gallic acid ameliorated the MTX-induced biochemical changes observed. Taken together, GA protected against MTX-induced hepatotoxicity and nephrotoxicity in rats, by reducing the impact of oxidative damage to tissues.

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Author Biography

Ayokanmi Ore, Biochemistry Unit, Department of Chemical Sciences, Ajayi Crowther University, Oyo, Oyo State
Biochemistry, Lecturer II
Published
2016-08-26
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Original Articles
Keywords:
Methotrexate, hepatotoxicity, nephrotoxicity, gallic acid, rat.
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How to Cite
Olayinka, E. T., Ore, A., Adeyemo, O. A., & Ola, O. S. (2016). Ameliorative effect of gallic acid on methotrexate-induced hepatotoxicity and nephrotoxicity in rat. Journal of Xenobiotics, 6(1). https://doi.org/10.4081/xeno.2016.6092