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Although the use of hazelnut oil is becoming ever more common, the ozonated form of this oil has never been obtained before. Ozonated vegetable oils are generally preferred in ozone therapy as they are easy to apply and obtain. In this study, the density, viscosity, peroxide and iodine values of hazelnut oil obtained by being treated with ozone in different periods at a flow rate of 7-8 were determined and compared with its commercial form, i.e. refined hazelnut oil. In order to determine whether ozonated hazelnut oil can be safely used, its cytotoxic effect potential was assessed by performing 3-[4,5-dimethylthiazol-2-yl]-2,5- diphenyltetrazolium assay on three different cell lines. For this purpose, the viability at five different concentrations of each sample was calculated after 48 and 72 hours of incubation in the H1299 lung cancer, human umbilical cord vein endothelial cells and A549 cell line. As a result, it was found that hazelnut oil applied to three different cell lines and in five different concentrations (100/50/25/12.5/6.25μg/mL) did not have any cytotoxic effects. Furthermore, it was also revealed that the samples did not have a toxic effect due to treatment with ozone in comparison to refined ozonated hazelnut oil. Therefore, this study has shown that ozonated hazelnut oil can be used as an alternative to refined hazelnut oil without exerting any toxic effects due to treatment with ozone. In light of this information, it was concluded that hazelnut oil can be safely used after treatment with ozone without having any cytotoxic effects and that it can be produced as a functional oil providing both the pharmacological and cosmetic effects of ozone.
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