TY - JOUR AU - Cariani, Lisa AU - Biffi, Arianna AU - Guarneri, Davide AU - Teri, Antonio AU - Girelli, Daniela AU - Vignati, Chiara AU - Morelli, Patrizia AU - Campana, Silvia AU - Dolce, Daniela AU - Iula, Vita Dora AU - Catania, Maria Rosaria AU - Cirilli, Natalia AU - Rapagnani, Sara AU - Rossitto, Martina AU - Fiscarelli, Ersilia Vita PY - 2021/05/06 Y2 - 2024/03/28 TI - Scedosporium species and Lomentospora prolificans in Italian cystic fibrosis patients: prevalence and distribution in seven centers using a selective medium JF - Microbiologia Medica JA - Microbiol Med VL - 36 IS - 1 SE - Original Articles DO - 10.4081/mm.2021.9335 UR - https://www.pagepressjournals.org/mm/article/view/9335 SP - AB - <p><em>Background and aims.</em> <em>Scedosporium</em> species and <em>Lomentospora</em> prolificans are the filamentous fungi isolated more frequently from the cystic fibrosis (CF) lower airways, after <em>Aspergillus</em> <em>fumigatus</em>. Previous studies showed that, in CF patients, <em>Scedosporium</em> species and <em>Lomentospora</em> prolificans are responsible for airways colonization/ infection, enhancing pulmonary deterioration, and for severe invasive infections in CF patients undergoing lung transplantation. Aims of our project were to evaluate the prevalence of <em>Scedosporium</em> and <em>Lomentospora</em> species in Italian patients with CF, and to evaluate the interest of the <em>Scedosporium</em>-selective colture medium SceSel compared to routinely used agar-based culture media, in order to improve laboratory diagnostic performances.<br><em>Materials and methods.</em> A total of 1977 sputum samples from 1154 CF patients were collected and processed according to CF Italian recommendations in the year 2017 (January 1st to December 31st). The SceSel agar was used in addition to the routine culture procedures to burst growth and isolation of <em>Scedosporium</em>/ <em>Lomentospora</em> species. The fungal isolates were identified by classical mycological methods and polymerase chain reaction-based DNA sequencing of ITS1 and ITS4 regions. Susceptibility of the isolates to antifungal drugs was investigated by E-test method. <br><em>Results</em>. Among the 1154 enrolled patients, 62 (5.3%) were positive for <em>Scedosporium/Lomentospora</em> species (median age: 27y; range 8-64y; 33 male). Out of 1977 samples, 93 were positive for <em>Scedosporium/Lomentospora</em> species, and 13 (13.9%) were recovered only on SceSel agar. According to molecular analyses, isolation rates of each species were:<em> S. apiospermum</em> 53.1%, <em>S. boydii</em> 37.5%, <em>L. prolificans</em> 6.3% and <em>S. aurantiacum</em> 3.1%. Amphotericin B minimum inhibitory concentration (MIC) values were above or equal to 2 mg/L in all strains. Voriconazole MIC values were below or equal to 0.38 mg/L in almost all strains, proving this drug to be the most effective antimycotic for members of Scedosporium species. Otherwise, Lomentospora prolificans showed to be resistant to all considered antifungal drugs, only Voriconazole seems to be active on some <em>Lomentospora</em> <em>prolificans</em> isolates. <br><em>Conclusions</em>. Our results suggest that SceSel agar should be used in combination with routine media as standard microbiological protocol and procedures are not always adequate to isolate Scedosporium and Lomentospora species in CF respiratory samples. Molecular identification and susceptibility tests are needed, especially for strains isolated from critical patients and those considered for transplantation, as they might develop invasive scedosporiosis.</p> ER -