Evaluation of a new system (RAD 120) for the serologic determination of the T.O.R.C.H complex analytes

  • Mauro Carcheri | mauro.carcheri@villascassi.it
  • Ilaria Chiossone
  • Paola Milano
  • Anna Alabiso
  • Fabrizia Capanna
  • Annamaria Ferretti
  • Licia Muselli
  • Caterina Oliveri
  • Laura Tassi
  • Roberto Capuzzo

Abstract

The aim of this study was to evaluate the results of the determination of IgG and IgM against TORCH complex antigens obtained by using a new automatic random-access (RAD 120 – RADIM/SEAC) instrument based on FEIA method, in comparison with the results obtained for the same analytes by using a traditional analyzer based on a coated microplate ELISA method (ALISEI-RADIM/SEAC).The analysis of the discordances between the results was made with the system ELFA (VIDAS – bioMérieux), taken as a reference test. The general concordance of the results obtained with the FEIA and the ELISA was over 97.00%. For the single analyte as Toxo IgG,Toxo IgM, Rubeo IgM, CMV IgG, CMV IgM and HSV 2 IgG, the concordance was above 96.00% and 100.00% for the Rubeo IgG and HSV 1 IgG. For the HSV 1/2 IgM, the FEIA/ELISA results show lower concordance, but still reaching 90.16%. For the IgG and IgM dosage, the FEIA seems to give more positive results than the ELISA. If the discordances FEIA/ELISA concern the IgG, the ELISA seems to agree better with the ELFA results. For the IgM dosage, all the FEIA positive/ELISA negative results have been confirmed as negative at ELFA. In conclusion the new technology FEIA/RAD120 gave results which can be comparable with those obtained at the traditional ELISA as well at the alternative ELFA.

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Published
2010-03-31
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Original Articles
Keywords:
Antigen, Antibody, IgG, IgM, T.O.R.C.H. complex
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How to Cite
Carcheri, M., Chiossone, I., Milano, P., Alabiso, A., Capanna, F., Ferretti, A., Muselli, L., Oliveri, C., Tassi, L., & Capuzzo, R. (2010). Evaluation of a new system (RAD 120) for the serologic determination of the T.O.R.C.H complex analytes. Microbiologia Medica, 25(1). https://doi.org/10.4081/mm.2010.2477

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