Comparison between two diagnostic system such analyzer I and RAD 120 for the determination of IgG and IgM for HSV-1 and HSV-2

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Annelisa Borelli *
Vincenzina Caruso
Maria Teresa Cerminara
Salvatore Nisticò
Vilma Villella
Pasquale Minchella
Rosa Anna Leone
(*) Corresponding Author:
Annelisa Borelli | lisi.borelli@libero.it

Abstract

Herpes Simplex Virus (Herpesviridae family, Alphaherpesviridae subfamily) induces latent infections, which could reactivate in conjunction with decreases in cell-mediated response. Features biologic and antigenic of Herpes Simplex virus are characterized from HSV-1 e HSV-2. Seronegative women can contract primary infection from seropositive partners. Seronegative women may acquire primary infection from an infected partner. Prevention is done paying attention to the risk of HSV2 in the planning and gravidnza pportune neonatal infection prevention measures (clinical examination of the birth canal at the beginning of labor). Disease prevention is performed by planning preventive measures to avoid neonatal infection. Infection occurs through direct contact with herpetic lesions or biological fluid infect. HSV-1 is usually acquired about 5 years, whether HSV-2 is contracted between 14 and 30 years by sexual contact, both can be asymptomatic and be transmitted to the partner or to the newborn during the delivery through the contact with infected secretion. The baby infected 85% of cases acquired the infection to step into the birth canal, through contact with infected secretions. The intrauterine infection is proven in 5-8% of cases; in the 8-10% of cases postnatal infection the disease spreading occurs through breast milk or herpes skin lesions.The assessment of immune status can be evaluated by different serological methods, for determinate the presence of IgG and / or IgM anti-HSV-1 and anti-HSV-2. Aim of this work is the comparison between two diagnostic systems such Analyzer I (Euroimmun) and RAD 120 (Radim) for the determination of IgG and IgM anti-HSV-1 and anti-HSV-2. Between July 2009 - December 2009 n. 182 samples have been tested with Analyzer I, automated ELISA system automatic, and system Rad 120, final fluorescent determination. From the obtained results we conclude that both the methods perform quit well.

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