Antibiotic susceptibility profile of Streptococcus pneumoniae isolated from acute respiratory infection in Dakar: a cross sectional study

  • Abdoulaye Diop | laycoumba@yahoo.fr Bacteriology and Virology Laboratory, Le Dantec University Teaching Hospital, Dakar, Senegal.
  • Makhtar Camara Bacteriology and Virology Laboratory, Le Dantec University Teaching Hospital, Dakar, Senegal.
  • Abdoulaye Seck Medical Virology Unit, Institute Pasteur, Dakar, Senegal.
  • Assane Dieng Bacteriology and Virology Laboratory, Le Dantec University Teaching Hospital, Dakar, Senegal.
  • Amadou Diop Bacteriology and Virology Laboratory, Le Dantec University Teaching Hospital, Dakar, Senegal.
  • Amary Fall Medical Virology Unit, Institute Pasteur, Dakar, Senegal.
  • Cherif Ibrahima. Khalil Diop Laboratory of Renewable Nanomaterial, University of Maine, Orono, ME, United States.
  • Djibril Boiro Paediatric Unit, Abass Ndao University Teaching Hospital, Dakar, Senegal.
  • Jean Baptisse Niokhor Diouf Paediatric Unit, Roi Baudouin Hospital, Dakar, Senegal.
  • Ibrahima Sene Bacteriology and Virology Laboratory, Le Dantec University Teaching Hospital, Dakar, Senegal.
  • Modou Gueye Paediatric Unit, Abass Ndao University Teaching Hospital, Dakar, Senegal.
  • Mbayame Niang Medical Virology Unit, Institute Pasteur, Dakar, Senegal.
  • Cheikh Saad Bouh Boye Bacteriology and Virology Laboratory, Le Dantec University Teaching Hospital, Dakar, Senegal.

Abstract

Streptococcus pneumoniae is a pathogen causing pneumonia, meningitis, otitis and bacteraemia. Nowadays, S. pneumoniae is developing antibacterial resistance, particularly for those with reduced susceptibility to penicillin. The objective of this study was to assess the susceptibility profile of S. pneumoniae strains isolated from acute respiratory infections (ARIs) in children younger than 5 years of age in Dakar, Senegal. S. pneumoniae strains were isolated from broncho-alveolar lavages (BALs), nasopharyngeal swabs, and middle ear secretion from children in the Paediatric Department of Abass Ndao University Teaching Hospital and Paediatric Department of Roi Baudouin Hospital in Dakar, Senegal. The strains were cultivated on Columbia agar supplemented with 5% of horse blood and gentamicin (6 mg/L). Antibiotic susceptibility testing was performed using E-test method. A total of 34 strains of S. pneumoniae were isolated and identified in this study, among them 7 strains (20.58%) showed penicillin-resistance. Antibiotics such as amoxicillin/clavulanic acid (MIC90=0.036 μg/mL), cefuroxim (MIC90=0.38 μg/mL), cefixim (MIC90=1.5 μg/mL), as well as macrolides (azithromycin MIC90=1.5 μg/mL, clarithromycin MIC90=0.125 μg/mL) and fluoroquinolone (levofloxacin MIC90=1 μg/mL, ofloxacin MIC90=2 μg/mL) were mostly active. However, all S. pneumoniae strains were resistant to sulfamethoxazole/trimethoprim (MIC90: 32 μg/mL). Except of S. pneumoniae strains penicillin-resistance or reduced susceptibility, most strains were susceptible to β-lactams antibiotics commonly used in ARI treatment. Continuous surveillance of antimicrobial resistance patterns of pneumococcus strains is still crucial for effective control of ARIs in children.

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Published
2018-12-19
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Original Articles
Supporting Agencies
GlaxoSmithKline (GSK)
Keywords:
Respiratory tract infections, Streptococcus pneumoniae, Antibiotic susceptibility
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How to Cite
Diop, A., Camara, M., Seck, A., Dieng, A., Diop, A., Fall, A., Diop, C., Boiro, D., Diouf, J., Sene, I., Gueye, M., Niang, M., & Boye, C. (2018). Antibiotic susceptibility profile of Streptococcus pneumoniae isolated from acute respiratory infection in Dakar: a cross sectional study. Microbiologia Medica, 33(1). https://doi.org/10.4081/mm.2018.7862

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