Antibiotic susceptibility profile of Streptococcus pneumoniae isolated from acute respiratory infection in Dakar: a cross sectional study
Streptococcus pneumoniae is a pathogen causing pneumonia, meningitis, otitis and bacteraemia. Nowadays, S. pneumoniae is developing antibacterial resistance, particularly for those with reduced susceptibility to penicillin. The objective of this study was to assess the susceptibility profile of S. pneumoniae strains isolated from acute respiratory infections (ARIs) in children younger than 5 years of age in Dakar, Senegal. S. pneumoniae strains were isolated from broncho-alveolar lavages (BALs), nasopharyngeal swabs, and middle ear secretion from children in the Paediatric Department of Abass Ndao University Teaching Hospital and Paediatric Department of Roi Baudouin Hospital in Dakar, Senegal. The strains were cultivated on Columbia agar supplemented with 5% of horse blood and gentamicin (6 mg/L). Antibiotic susceptibility testing was performed using E-test method. A total of 34 strains of S. pneumoniae were isolated and identified in this study, among them 7 strains (20.58%) showed penicillin-resistance. Antibiotics such as amoxicillin/clavulanic acid (MIC90=0.036 μg/mL), cefuroxim (MIC90=0.38 μg/mL), cefixim (MIC90=1.5 μg/mL), as well as macrolides (azithromycin MIC90=1.5 μg/mL, clarithromycin MIC90=0.125 μg/mL) and fluoroquinolone (levofloxacin MIC90=1 μg/mL, ofloxacin MIC90=2 μg/mL) were mostly active. However, all S. pneumoniae strains were resistant to sulfamethoxazole/trimethoprim (MIC90: 32 μg/mL). Except of S. pneumoniae strains penicillin-resistance or reduced susceptibility, most strains were susceptible to β-lactams antibiotics commonly used in ARI treatment. Continuous surveillance of antimicrobial resistance patterns of pneumococcus strains is still crucial for effective control of ARIs in children.
Adela G, Balsalobre L, Ardanuy C, et al. Fluoroquinolone Resistance in Penicillin-resistant Streptococcus pneumoniae Clones, Spain. Emerg Infect Dis 2004; 10: 1751–1759.
Ahmadi A, Yaghoubi S, Irajian G. Molecular Analysis of PBP1A in Streptococcus pneumoniae Isolated from Clinical and Normal Flora Samples in Tehran, Iran: A Multicenter Study. MICROBIAL DRUG RESISTANCE 2018; 00.
Camara M, Dieng A, Diop A, et al. Antibiotic resistance of bacteria responsible of acute respiratory tract infections in children. Microbiologia Medica 2017; 32: 6489.
Elmdaghri N, Benbachir M, Belabbes H, et al. Changing epidemiology of pediatric Streptococcus pneumoniae isolates before vaccine introduction in Casablanca (Morocco). Vaccine 2012; 30:46-50.
Gueye A , Boye CS , Hounkponou1 E ,et al . Antimicrobial susceptibility of select respiratory tract pathogens in Dakar, Senegal. J Infect Dev Ctries 2009. 3 :660-6. 5
Hecini-Hannachi A, Bentchouala C, Lezzar A, et al. Serotypes and antimicrobial resistance of invasive Streptococcus pneumoniae isolates from East Algeria (2005-2011). Afr J Microbial Res 2013; 8:167-77.
Holliman RE , Liddy H, Johnson JD, et al. Epidemiology of invasive pneumococcal disease in Kumasi, Ghana. Trans R Soc Trop Med Hyg 2007; 101:405-13.
Jacobs MR, Felmingham D, Appelbaum PC, et al. The Alexander Project 1998-2000: susceptibility of pathogens isolated from community-acquired respiratory tract infection to commonly used antimicrobial agents. J Antimicrob Chemother. 2003; 229-46.
Kaijalainen T. The Identification of Streptococcus pneumoniae. The National Public Health Institute 2006; 76.
Karcic E, Aljicevic M, Bektas S, et al. Antimicrobial Susceptibility/Resistance of Streptococcus Pneumoniae. Mater Sociomed 2015; 27: 180–184.
Karpanoja P . Antimicrobial Resistance in The Major Respiratory Tract Pathogens – Methods and Epidemiology. Faculty of Biological and Environmental Sciences .Department of Biosciences. University of Helsinki Academic Dissertation 2017; 30-31.
Ktari S, Ben Ayed N, Jmal I, et al. Clinical levofloxacin resistant Streptococcus pneumoniae isolates in North Africa. Global Antimicrobial Resistance 2018; 12: 181-182.
Mathur S, Fuchs A, Bielicki J, et al. Antibiotic use for community-acquired pneumonia in neonates and children: WHO evidence review Paediatrics and International Child Health 2018; 38: 66–75.
Raddaoui A, Ben Tanfous F, Chebbi Y, et al. High prevalence of multidrug resistant international clones among macrolide resistant Streptococcus pneumoniae strains in immunocompromised Patients in Tunisia. Int J Antimicrob Agents 2018; 0924-8579: 30117-1.
Robert P. Fluoroquinolone Positioning in Hospital Antimicrobial Stewardship Programs. US PHARM 2007; 32: 10 -17.
Save the Children. Fighting For Breath: A call to action on childhood pneumonia. Report 2017; 1.
Sener B, Tunçkanat F, Ulusoy S, et al. A survey of antibiotic resistance in Streptococcus pneumoniae and Haemophilus influenzae in Turkey, 2004–2005. J Antimicrob Chemother 2007; 60:587-93.
Shibl A, Daniels J, Sievers J. Antimicrobial resistance among Streptococcus pneumoniae and Haemophilus influenzae from Africa and the Middle East: 2002/2003 winter season. Clin Microbiol Infect 2004 ; 10:111.
Silberbaue EJ, Ismai N, Von-Gottberg A, et al. Serotype and antimicrobial profile distribution of invasive pneumococcal isolates in the pre-vaccine introduction era in Pretoria, South Africa 2005 through 2009. Diagn Microbiol Infect Dis 2011;71: 309-11.
Smaoui H, Amri J, Hajji N, et al. Antimicrobial susceptibility and serotype distribution of Streptococcus pneumoniae isolates in children in Tunis. Arch Pediatr 2009 ; 6 : 220-6.
USAID/ MCHIP. Integrated Community Case Management of Childhood Illness: Documentation of Best Practices and Bottlenecks to Program Implementation in Senegal. Revised and Submitted 2 September 2011, 1.
Vardakas KZ, Siempos II, Grammatikos A , et al. Respiratory fluoroquinolones for the treatment of community-acquired pneumonia: a meta-analysis of randomized controlled trials. CMAJ 2008 ; 179 :1269-77.
World Health Organization. Revised WHO classification and treatment of childhood pneumonia at health dacilities – evidence summaries. Geneva: WHO; 2015.
Zhang Y, Zhang F, Hui Wang H, et al. Antimicrobial susceptibility of Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis isolated from community-acquired respiratory tract infections in China: Results from the CARTIPS Antimicrobial Surveillance Program. Journal of Global Antimicrobial Resistance 2016; 36–41.
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Copyright (c) 2018 Abdoulaye Diop, Makhtar Camara, Abdoulaye Seck, Assane Dieng, Amadou Diop, Amary Fall, Cherif Ibrahima. Khalil Diop, Djibril Boiro, Jean Baptisse Niokhor Diouf, Ibrahima Sene, Modou Gueye, Mbayame Niang, Cheikh Saad Bouh Boye
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