Detection of enterotoxins and genotyping of Staphylococcus aureus strains isolated from Isfahan Educational Hospital, Iran
Submitted: 29 July 2017
Accepted: 6 September 2017
Published: 10 October 2017
Accepted: 6 September 2017
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Materials and methods: In the present study during the sixmonths sampling, fifty S. aureus strains were isolated from patients admitted to Al-Zahra university hospital. Antimicrobial susceptibility testing, Multiplex PCR for detection of enterotoxin A, C and TSST-1, pulse field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were used for molecular typing.
Results: In antibiogram the highest and lowest percentage of resistance was belonged to tetracycline and rifampin respectively. Multiplex PCR indicated that 30% of the strains harbored sea and 34% harbored sec genes. However, only 4% of our collected isolates had tsst gene. In PFGE method analysis on all S. aureus strains, a total of 19 different patterns were identified. Nine various sequence types in 27 selected S. aureus isolates were identified by MLST.
Conclusions: Present study indicates a possible higher variability among our S. aureus strains by two different molecular typing methods; nevertheless four main common types (CT1, CT7, CT9, and CT11) with at least one toxin genes were determined.
Supporting Agencies
This study was funded by the department of microbiology of Isfahan University of Medical Sciences.Havaei, S. A., Rezaei, N., Havaei, R., & Namvar, A. E. (2017). Detection of enterotoxins and genotyping of Staphylococcus aureus strains isolated from Isfahan Educational Hospital, Iran. Microbiologia Medica, 32(3). https://doi.org/10.4081/mm.2017.6965
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