Impact of two different commercial DNA extraction methods on BK virus viral load
Submitted: 11 November 2014
Accepted: 28 January 2016
Published: 31 March 2016
Accepted: 28 January 2016
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Materials and methods: DNA extraction procedure including the Nuclisens easyMAG platform (bioMerieux, Marcy l’Etoile, France) and manual QIAGEN extraction (QIAGEN Hilden, Germany). BK DNA quantification was performed by Real Time TaqMan PCR using a commercial kit.
Result and discussion: The samples capacity, cost and time spent were compared for both systems. In conclusion our results demonstrate that automated nucleic acid extraction method using Nuclisense easyMAG was superior to manual protocol (QIAGEN Blood Mini kit), for the extraction of BK virus from serum and urine specimens.
Bergallo, M., Galliano, I., Loiacono, E., Ferro, F., Montanari, P., & Ravanini, P. (2016). Impact of two different commercial DNA extraction methods on BK virus viral load. Microbiologia Medica, 31(1). https://doi.org/10.4081/mm.2016.4825
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