Impiego del sistema Uro-Quick per l’identificazione di resistenze ben caratterizzate agli antibiotici veicolate da diverse specie batteriche
AbstractThe Uro-Quick system, previously used to carry out antibiotic susceptibility tests directly on urine samples, has been employed for the detection of antibiotic resistance traits that might be not easily detected in well-characterized bacterial species.The period of time requested by the instrument to identify the drug resistance were: penicillin-resistant (PEN-R), constitutive and inducible oxacillin-resistant (OXA-R) Staphylococcus aureus, OXA-R S. epidermidis 480 min.Teicoplanin-resistant (TEI-R) S. haemolyticus, Vancomycin- (VanA,VanB,VanC), aminoglycoside- resistant (HLAR) Enterococcus spp, 24h. S. pyogenes exhibiting the three erythromycin-resistance phenotypes (Ery-R,C, I e M) 480 min.Ampicillin- (AMP-R), ciprofloxacin (CIP-R) and clavulanic acid-resistant (IRT) Escherichia coli 180 min. Imipenem-resistant (IMI-R) Pseudomonas aeruginosa 24h. For each strain was evaluated the optimal experimental conditions including inoculum size, time of incubation and antibiotic concentration. The results obtained indicated that Uro-Quick might be employed for the correct detection of the above antibiotic resistant markers.
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Copyright (c) 2003 Simona Roveta, Anna Marchese, Eugenio A. Debbia
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