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Numerous transport and growth medium systems have been developed for enhanced growth of genital mycoplasmas. In this investigation a total of 161 patient specimens were cultured on the Urée-Arginine LYO 2 screening broth from the Mycoplasma IST 2 kit, on the U.M.M.lyo regenerated screening broth from the Mycofast Screening EvolutioN 2 kit and on A7 agar as control of Mycoplasma hominis and Ureaplasma urealyticum culture. Rates of recovery of these organisms with commercial kits were compared with the standard culture on A7 agar. We evaluated the performance of both screening test: a) Urée-Arginine LYO 2 screening broth test sensitivity was 100%, specificity 96.2%, positive predictive value 93.2% and negative predictive value100%; b) U.M.M.lyo regenerated screening broth test sensitivity was 80%, specificity 100%, positive predictive value 100% and negative predictive value 90.6%. The results of the identification and enumeration obtained on A7 agar complied a) with Mycoplasma IST 2 strip for 100% of M. hominis and 85% of U. urealyticum b) with Mycofast Screening EvolutioN 2 strip for 50% of M. hominis and 100% of U. urealyticum. According our study Urée-Arginine LYO 2 screening broth is an optimal medium for the recovery of M. hominis and U. urealyticum. Moreover we confirmed the screening positive tests evaluating sensitivity and specificity through the agar test culture.The Mycoplasma IST 2 strip gives an indicative enumeration and for this reason it is necessary a sub-culture on A7 agar for any positive screening. This study shows that Mycofast Screening EvolutioN 2 does not allow an optimal recovery of the genital micoplasmas, particularly Mhominis and for this reason we recommend the combination of Mycofast Screening EvolutioN 2 with A7 agar. We have also studied susceptibilities of M. hominis and U. urealyticum to different antibiotics and their association with G. vaginalis.
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