Valutazione di un test per la determinazione simultanea degli anticorpi e antigene p24 dell’HIV

Nadia Zanchetta; Carla Bossi; Claudia Tonielli; Claudio Galli

doi:10.4081/mm.2006.2954

Valutazione di un test per la determinazione simultanea degli anticorpi e antigene p24 dell’HIV

https://doi.org/10.4081/mm.2006.2954

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Vol. 21 No. 1 (2006)

Submitted: 24 February 2014
Accepted: 24 February 2014

Published: 31 March 2006

HIV, 4th generation assay, p24, anti-HIV, screening

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Authors

A fourth generation immunoassay for the detection of antibodies to HIV-1 and HIV-2 and of HIV antigen p24 (Abbott Architect HIV Ag/Ab Combo) has been evaluated in comparison with two Ab-only third generation assays on 894 routine specimens and on preselected repository specimens.The Combo assay showed a better specificity (99.88% vs. 99.43-99.83%) and an analytical sensitivity for p24 of 22 pg/mL on a BBI commercial panel. The Architect assays gave a negative result on 22/24 repository false positive specimens from 18 subjects and, conversely, was positive on all the 39 repository specimens from 24 HIV-positive patients. On six patients with acute HIV infection the Architect assay gave an earlier positivity than the antibody-only assays (EIA and WB) on three cases, all viremic and positive for HIV p24.The performance characteristics of the new HIV Combo assay guarantee an advanced clinical sensitivity and a high specificity.

Zanchetta, N., Bossi, C., Tonielli, C., & Galli, C. (2006). Valutazione di un test per la determinazione simultanea degli anticorpi e antigene p24 dell’HIV. Microbiologia Medica, 21(1). https://doi.org/10.4081/mm.2006.2954