Microscopie 2020-06-26T12:53:29+00:00 Nadia Moscato Open Journal Systems <h3>Italian Society for Microscopical Sciences - <a href="" target="_blank" rel="noopener"></a></h3> <p><strong>Microscopie</strong>&nbsp;is the official organ of the <em>Italian Society for Microscopical Sciences</em> (SISM).&nbsp;The Journal&nbsp;was founded in 1954 and published until 2004 under the title "Microscopia Elettronica".</p> <p><strong>Microscopie</strong> presently publishes original articles concerning <strong>any kind of microscopical and imaging techniques</strong>, in the field of <strong>Life and Material Sciences, </strong>with special attention to articles on newly developed or originally applied microscopical techniques. The Journal also publishes information on the Society activities, and advertisements of the main Italian and international events on microscopical sciences.&nbsp;</p> <p>The journal accepts scientific manuscripts as well as reports on new instruments and technological applications by authors from public institutions or private companies of any Country, irrespective of whether or not they are fellows of the Italian Society for Microscopical Sciences.</p> <p><strong>Microscopie</strong> aims to be a forum for all those scientists who are active in the field of microscopy, and is especially intended as a platform for young microscopists at the beginning of their scientific career. <strong>Thus a</strong><strong>ll the submitted manuscripts that are accepted after peer-reviewing will be published free of charge</strong>.</p> Dermal ultrastructure in a case of Parry-Romberg syndrome 2020-06-26T12:53:29+00:00 Federica Boraldi Francesco Demetrio Lofaro Daniela Quaglino <p>A case of Parry Romberg syndrome (PRS) in a 7-year-old girl is described. Ultrastructural investigations, supported by clinical evaluations, were performed on both affected and unaffected skin. Connective tissue abnormalities were mainly observed in the diseased area, where an increased number of mast cells can be observed, and collagen is organized in large bundles with fibrils of heterogeneous diameters. Data are suggestive for increased matrix remodeling in the affected skin.</p> 2020-06-26T12:53:28+00:00 ##submission.copyrightStatement## Nanotechnology and nanomaterials in the strategy for the treatment of neoplastic diseases 2020-06-25T09:22:53+00:00 Maria Condello Stefania Meschini <p>Nanoparticles (NPs) have nanometric dimensions, a large surface-volume ratio, physical and chemical stability and individual optical electronic properties. These characteristics have allowed the use of nanostructured materials for the prevention and treatment of various diseases, such as cancer. NPs have been designed and modified to improve the pharmacokinetics and pharmacodynamics of drugs, and to target drugs to cancer cells only. Various nanomaterials can be used for nanomedical applications. In particular, inorganic NPs, such as zinc oxide (ZnO-NPs), gold (Au-NPs) and silver (Ag-NPs) NPs, have been used to improve anticancer therapies. Biosynthesized inorganic NPs were loaded with chemotherapeutic drugs and subsequently functionalized to selectively target cancer cells. Many studies have identified the cellular mechanisms involved after cell-NP interaction: oxidative stress, mitochondrial alterations, lysosomal dysfunction, apoptosis or alternatively autophagy. To improve knowledge of the interaction between drugs loaded on NPs and cells and optimize their use by reducing toxic effects, transmission electron microscopy (TEM) techniques proved to be a good investigation tool. TEM observations have shown, for example, that ZnO-NPs enter the cells by passive diffusion or endocytosis. Ultrastructural analysis showed that Au-NPs enter the cells by invagination of the plasma membrane and are subsequently internalized in the autophagosome. This brief review shows that each new NP needs to be evaluated individually considering all its properties.</p> 2020-06-25T09:22:52+00:00 ##submission.copyrightStatement## Scanning and transmission electron microscopy in cultural heritage: State of the art 2020-06-23T12:27:18+00:00 Sabrina Burattini Elisabetta Falcieri <p>The aim of this review is to analyse and report the importance of scanning and transmission electron microscopy applied to cultural heritage. Even if a lot of work has been carried out by chemical and microanalytical methods, the contribute of the morphological study has been only recently focused. In particular, scanning electron microscopy (SEM), requiring easier specimen preparations, has been widely applied to the study of ancient wood, textiles, metal or lithic objects, giving precious information on their surface details, manufacturing or possible contaminating agents. On the other hand, transmission electron microscopy (TEM) analysis, more difficult due to sample embedding and sectioning, can provide important details mainly on wood and textile samples. Both techniques are becoming progressively more useful for both cultural heritage study and diagnostic.</p> 2020-06-23T12:27:17+00:00 ##submission.copyrightStatement##